Cited by 235Gene Regulatory Network AnalysisGenomics and Chromatin DynamicsBacterial Genetics and Biotechnology

Transcription in Living Cells: Molecular Mechanisms of Bursting

Rodriguez, Joseph, Larson, Daniel R.·2020·Annual Review of Biochemistry

Abstract

Transcription in several organisms from certain bacteria to humans has been observed to be stochastic in nature: toggling between active and inactive states. Periods of active nascent RNA synthesis known as bursts represent individual gene activation events in which multiple polymerases are initiated. Therefore, bursting is the single locus illustration of both gene activation and repression. Although transcriptional bursting was originally observed decades ago, only recently have technological advances enabled the field to begin elucidating gene regulation at the single-locus level. In this review, we focus on how biochemical, genomic, and single-cell data describe the regulatory steps of transcriptional bursts.

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Crossref · 10.1146/annurev-biochem-011520-105250

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161 of 161 references verified0 citation errors · 0 not checkable

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  • In eukaryotic cells, transcription occurs in cycles.

    “In eukaryotic cells, transcription occurs in cycles [50,51,52].”

    From Enhancer Function in the 3D Genome (2023) · cites this paper as [52]

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Authors
Rodriguez, J., & Larson, D. R.
Affiliations
  • National Institute of Environmental Health Sciences (US)
  • National Cancer Institute (US)
Venue
Annual Review of Biochemistry
Published
2020-06-20
Volume
89
Issue
1
Pages
189-212
ISSN
  • 0066-4154 (ISSN-L)
  • 1545-4509
OpenAlex
W3013821085
Cited by
224
References
161
Citations by year
2026: 19 · 2025: 32 · 2024: 50 · 2023: 38 · 2022: 29 · 2021: 42 · 2020: 14
FWCI
3.06
Type
journal article
Language
en
Open access
closed
Locations
  • Annual Review of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
transcriptional bursting, stochastic gene expression, gene activation, gene repression, RNA polymerase, single-cell transcriptomics, transcriptional noise
MeSH terms
Animals, Chromatin, DNA, Eukaryotic Cells, Gene Expression Regulation, Histones, Humans, RNA Polymerase II, RNA, Messenger, Stochastic Processes, Transcription, Genetic, Molecular Probes, Molecular Probe Techniques, Genome, Genetic Loci, Single-Cell Analysis

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