Cited by 292CRISPR and Genetic EngineeringRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry

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In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence

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Choo, Yen, Sánchez-García, Isidro, Klug, Aaron·1994·Nature

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Abstract

A DNA-binding peptide comprising three zinc-fingers has been engineered to bind specifically to a unique nine-base-pair region of a BCR-ABL fusion oncogene in preference to the parent genomic sequences. Binding to the target oncogene in chromosomal DNA is possible in transformed cells in culture, and results in blockage of transcription. Consequently, murine cells rendered independent of growth factors by the action of the oncogene revert to factor dependence upon transient transfection with a vector expressing the peptide.

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Authors: Choo, Y., Sánchez-García, I., & Klug, A.
Affiliations: MRC Laboratory of Molecular Biology (GB)
ORCID: Choo, Yen
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 1994-12
Volume: 372
Issue: 6507
Pages: 642-645
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/372642a0
OpenAlex: W2019616754
Cited by: 292
References: 36
Citations by year: 2026: 1 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 9 · 2019: 3 · 2018: 3 · 2017: 7 · 2016: 9 · 2015: 9 · 2014: 11 · 2013: 1 · 2012: 6
FWCI: 7.35
Type: journal article
Language: en
Open access: green
hdl.handle.net
Licence: other-oa
www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords: zinc finger DNA-binding protein, transcriptional repression, site-specific DNA binding, growth factor independence, transformed cells, transcriptional block
MeSH terms: Amino Acid Sequence, Animals, Cell Line, DNA, DNA-Binding Proteins, Feasibility Studies, Gene Expression Regulation, Humans, Molecular Sequence Data, Protein Binding, Recombinant Fusion Proteins, Transcription, Genetic, Transfection, Fusion Proteins, bcr-abl, Zinc Fingers, Mice

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

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In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence

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