Cited by 450Enzyme Structure and FunctionAmino Acid Enzymes and MetabolismMetal-Catalyzed Oxygenation Mechanisms

The DOI matches its Crossref record; 24 of the 26 references it cites were found in a registry or an index.

Structure of a unique binuclear manganese cluster in arginase

Abstract

Each individual excretes roughly 10 kg of urea per year, as a result of the hydrolysis of arginine in the final cytosolic step of the urea cycle. This reaction allows the disposal of nitrogenous waste from protein catabolism, and is catalysed by the liver arginase enzyme. In other tissues that lack a complete urea cycle, arginase regulates cellular arginine and ornithine concentrations for biosynthetic reactions, including nitric oxide synthesis: in the macrophage, arginase activity is reciprocally coordinated with that of NO synthase to modulate NO-dependent cytotoxicity. The bioinorganic chemistry of arginase is particularly rich because this enzyme is one of very few that specifically requires a spin-coupled Mn2+-Mn2+ cluster for catalytic activity in vitro and in vivo. The 2.1 angstrom-resolution crystal structure of trimeric rat liver arginase reveals that this unique metal cluster resides at the bottom of an active-site cleft that is 15 angstroms deep. Analysis of the structure indicates that arginine hydrolysis is achieved by a metal-activated solvent molecule which symmetrically bridges the two Mn2+ ions.

Overview

Integrity signals

Registry

Crossref · 10.1038/383554a0

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 26 references

Research works printed but not linked: 3

  1. Rardin, R. L., Tolman, W. B. & Lippard, S. J. New J. Chem. 15, 417–430 (1991).
  2. Nyborg, J. & Wonacott, A. J. in The Rotation Method in Crystallography (eds Arndt, U. W. & Wonacott, A. J.) 139–152 (North-Holland, Amsterdam, 1977).
  3. Furey, W. & Swaminathan, S. Am. Crystallogr. Assoc. Mtg Prog. Abstr. 18, 73 (1990).

Cited by

Sorted by citations

Metadata

Authors
Kanyo, Z. F., Scolnick, L. R., Ash, D. E., & Christianson, D. W.
Affiliations
  • University of Pennsylvania (US)
  • Temple University (US)
Venue
Nature
Published
1996-10
Volume
383
Issue
6600
Pages
554-557
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2066916400
Cited by
441
References
26
Citations by year
2026: 18 · 2025: 6 · 2024: 18 · 2023: 12 · 2022: 9 · 2021: 8 · 2020: 17 · 2019: 15 · 2018: 15 · 2017: 14 · 2016: 8 · 2015: 13 · 2014: 11 · 2013: 18 · 2012: 15
FWCI
15.87
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01GM067788)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
Keywords
arginase, urea cycle, arginine hydrolysis, crystal structure, metalloenzyme active site, nitric oxide production, enzyme catalysis
MeSH terms
Animals, Arginase, Arginine, Binding Sites, Liver, Manganese, Metalloproteins, Models, Molecular, Protein Conformation, Protein Structure, Secondary, Crystallography, X-Ray, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review