Cited by 299DNA Repair MechanismsT-cell and B-cell ImmunologyGenomics and Chromatin Dynamics

Fus deficiency in mice results in defective B-lymphocyte development and activation, high levels of chromosomal instability and perinatal death

Hicks, Geoffrey G., Singh, Nagendra, Nashabi, Abudi, Mai, Sabine, Bozek, Gracjan, Klewes, Ludger, Arapovic, Djula, White, Erica K. +4 more·2000·Nature Genetics

Abstract

The gene FUS (also known as TLS (for translocated in liposarcoma) and hnRNP P2) is translocated with the gene encoding the transcription factor ERG-1 in human myeloid leukaemias. Although the functions of wild-type FUS are unknown, the protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. FUS resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein and interacts with several transcription factors in vitro. To better understand FUS function in vivo, we examined the consequences of disrupting Fus in mice. Our results indicate that Fus is essential for viability of neonatal animals, influences lymphocyte development in a non-cell-intrinsic manner, has an intrinsic role in the proliferative responses of B cells to specific mitogenic stimuli and is required for the maintenance of genomic stability. The involvement of a nuclear riboprotein in these processes in vivo indicates that Fus is important in genome maintenance.

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Crossref · 10.1038/72842

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Authors
Hicks, G. G., Singh, N., Nashabi, A., Mai, S., Bozek, G., Klewes, L., Arapovic, D., White, E. K., Koury, M. J., Oltz, E. M., Van Kaer, L., & Ruley, H. E.
Affiliations
  • University of Manitoba (CA)
  • Howard Hughes Medical Institute (US)
  • Vanderbilt University (US)
Corresponding author
Hicks, Geoffrey G.
Venue
Nature Genetics
Published
2000-02
Volume
24
Issue
2
Pages
175-179
ISSN
  • 1061-4036 (ISSN-L)
  • 1546-1718
OpenAlex
W2080964315
Cited by
299
References
31
Citations by year
2026: 5 · 2025: 3 · 2024: 4 · 2023: 10 · 2022: 17 · 2021: 19 · 2020: 10 · 2019: 17 · 2018: 21 · 2017: 10 · 2016: 26 · 2015: 12 · 2014: 18 · 2013: 23 · 2012: 13
FWCI
2.51
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Genetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (2r01hg000684-04a1)
  • National Human Genome Research Institute (R01HG00684)
  • National Cancer Institute (P30CA042014)
  • National Center for Research Resources (R01RR013166)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
FUS gene, TLS, B cell development, B cell activation, chromosomal instability, genome stability, perinatal lethality, nuclear ribonucleoprotein, transcription factors
MeSH terms
Animals, Animals, Newborn, B-Lymphocytes, Bone Marrow Cells, Chimera, Crosses, Genetic, Female, Genotype, Humans, Liver, Lymphocyte Activation, Male, Mice, Inbred C57BL, Ribonucleoproteins, Spleen, Restriction Mapping, RNA-Binding Proteins, Mice, Knockout, Heterogeneous-Nuclear Ribonucleoproteins, RNA-Binding Protein FUS, Mice

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