The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter
Open accessAbstract
DNA-binding domains with predetermined sequence specificity are engineered by selection of zinc finger modules using phage display, allowing the construction of customized transcription factors. Despite remarkable progress in this field, the available protein-engineering methods are deficient in many respects, thus hampering the applicability of the technique. Here we present a rapid and convenient method that can be used to design zinc finger proteins against a variety of DNA-binding sites. This is based on a pair of pre-made zinc finger phage-display libraries, which are used in parallel to select two DNA-binding domains each of which recognizes given 5 base pair sequences, and whose products are recombined to produce a single protein that recognizes a composite (9 base pair) site of predefined sequence. Engineering using this system can be completed in less than two weeks and yields proteins that bind sequence-specifically to DNA with Kd values in the nanomolar range. To illustrate the technique, we have selected seven different proteins to bind various regions of the human immunodeficiency virus 1 (HIV-1) promoter.
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Cites
In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence
Choo, Y., Sánchez-García, I., & Klug, A. (1994) · Nature · doi:10.1038/372642a0 · Cited by 292 · cited 1×
“In contrast to these methods, we now present a zinc finger engineering system that (1) yields zinc finger polymers that bind DNA specifically, tightly, and without significant sequence restrictions, (2) can be completed relatively rapidly, and (3) can be easily adapted to a high-throughput automated format.”
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Cited by
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Urnov, F. D., Miller, J. C., Lee, Y.-L., Beausejour, C. M., Rock, J. M., Augustus, S., Jamieson, A. C., Porteus, M. H., Gregory, P. D., & Holmes, M. C. (2005) · Nature · doi:10.1038/nature03556 · Cited by 1,641
An improved zinc-finger nuclease architecture for highly specific genome editing
Miller, J. C., Holmes, M. C., Wang, J., Guschin, D. Y., Lee, Y.-L., Rupniewski, I., Beausejour, C. M., Waite, A. J., Wang, N. S., Kim, K. A., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1319 · Cited by 1,051
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
Perez, E. E., Wang, J., Miller, J. C., Jouvenot, Y., Kim, K. A., Liu, O., Wang, N., Lee, G., Bartsevich, V. V., Lee, Y.-L., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1410 · Cited by 999
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
Shukla, V. K., Doyon, Y., Miller, J. C., DeKelver, R. C., Moehle, E. A., Worden, S. E., Mitchell, J. C., Arnold, N. L., Gopalan, S., Meng, X., et al. (2009) · Nature · doi:10.1038/nature07992 · Cited by 958
Metadata
- Authors
- Isalan, M., Klug, A., & Choo, Y.
- Venue
- Nature Biotechnology
- Published
- 2001-07
- Volume
- 19
- Issue
- 7
- Pages
- 656-660
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1582870526
- Cited by
- 191
- References
- 1
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- green
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