The DOI matches its Crossref record; 32 of the 33 references it cites were found in a registry or an index.
Regulation of the cell cycle gene, BTG2, by miR-21 in human laryngeal carcinoma
Abstract
MicroRNAs are short regulatory RNAs that negatively modulate gene expression at the post-transcriptional level, and are deeply involved in the pathogenesis of several types of cancers. To investigate whether specific miRNAs and their target genes participate in the molecular pathogenesis of laryngeal carcinoma, oligonucleotide microarrays were used to assess the differential expression profiles of microRNAs and mRNAs in laryngeal carcinoma tissues compared with normal tissues. The oncogenic miRNA, microRNA-21 (miR-21), was found to be upregulated in laryngeal carcinoma tissues. Knockdown of miR-21 by specific antisense oligonucleotides inhibited the proliferation potential of HEp-2 cells, whereas overexpression of miR-21 elevated growth activity of the cells, as detected by the colony formation assay. The cell number reduction caused by miR-21 inhibition was due to the loss of control of the G1-S phase transition, instead of a noticeable increase in apoptosis. Subsequently, a new target gene of miR-21, BTG2, was found to be downregulated in laryngeal carcinoma tissues. BTG2 is known to act as a pan-cell cycle regulator and tumor suppressor. These findings indicate that aberrant expression of miR-21 may contribute to the malignant phenotype of laryngeal carcinoma by maintaining a low level of BTG2. The identification of the oncogenic miR-21 and its target gene, BTG2, in laryngeal carcinoma is potentially valuable for cancer diagnosis and therapy.
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“In laryngeal carcinoma, miR-21 targets BTG2 (Liu et al., 2009), a p53 target gene, recently shown to suppress oncogenic Ras activity by reducing the GTP bound, active state (Buganim et al., 2010).”
From Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21 (2010) · cites this paper as Liu et al., 2009
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Cites
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Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs
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MicroRNA Gene Expression Deregulation in Human Breast Cancer
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MicroRNA-21 Regulates Expression of the PTEN Tumor Suppressor Gene in Human Hepatocellular Cancer
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Silencing of microRNA families by seed-targeting tiny LNAs
Obad, S., dos Santos, C. O., Petri, A., Heidenblad, M., Broom, O. J., Ruse, C. I., Fu, C., Lindow, M., Stenvang, J., Straarup, E. M., et al. (2011) · Nature Genetics · doi:10.1038/ng.786 · Cited by 644
Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21
Hatley, M. E., Patrick, D. M., Garcia, M. R., Richardson, J. A., Bassel-Duby, R., van Rooij, E., & Olson, E. N. (2010) · Cancer Cell · doi:10.1016/j.ccr.2010.08.013 · Cited by 626
“In laryngeal carcinoma, miR-21 targets BTG2 (Liu et al., 2009), a p53 target gene, recently shown to suppress oncogenic Ras activity by reducing the GTP-bound, active state (Buganim et al.”
Zhang, J.-G., Wang, J., Zhao, F., Liu, Q., Jiang, K., & Yang, G. (2010) · Clinica Chimica Acta · doi:10.1016/j.cca.2010.02.074 · Cited by 574
MicroRNAs: Potential biomarkers for cancer diagnosis, prognosis and targets for therapy
Cho, W. C. S. (2009) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2009.12.014 · Cited by 487
Metadata
- Authors
- Liu, M., Wu, H., Liu, T., Li, Y., Wang, F., Wan, H., Li, X., & Tang, H.
- Affiliations
- Tianjin Medical University (CN)
- Corresponding author
- Tang, Hua
- Venue
- Cell Research
- Published
- 2009-06-23
- Volume
- 19
- Issue
- 7
- Pages
- 828-837
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- OpenAlex
- W2083966895
- Cited by
- 189
- References
- 33
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 6 · 2021: 18 · 2020: 11 · 2019: 4 · 2018: 11 · 2017: 10 · 2016: 14 · 2015: 20 · 2014: 20 · 2013: 8 · 2012: 14
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30873017)
- Tianjin Medical University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Health Sciences › Medicine › Otorhinolaryngology › Head and Neck Cancer Studies
- Keywords
- miR-21, BTG2, laryngeal cancer, miRNA target genes, cell cycle regulation, G1/S transition, tumor suppressor, cell proliferation, HEp-2 cells, post-transcriptional regulation, oncogenic microRNA
- MeSH terms
- Carcinoma, Humans, Laryngeal Neoplasms, Down-Regulation, Gene Expression Regulation, Neoplastic, G1 Phase, S Phase, Oligonucleotides, Antisense, Immediate-Early Proteins, Tumor Suppressor Proteins, MicroRNAs, Cell Line, Tumor, Gene Knockdown Techniques
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