MicroRNA Gene Expression Deregulation in Human Breast Cancer
Published version of a preprint
Abstract
MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Their aberrant expression may be involved in human diseases, including cancer. Indeed, miRNA aberrant expression has been previously found in human chronic lymphocytic leukemias, where miRNA signatures were associated with specific clinicobiological features. Here, we show that, compared with normal breast tissue, miRNAs are also aberrantly expressed in human breast cancer. The overall miRNA expression could clearly separate normal versus cancer tissues, with the most significantly deregulated miRNAs being mir-125b, mir-145, mir-21, and mir-155. Results were confirmed by microarray and Northern blot analyses. We could identify miRNAs whose expression was correlated with specific breast cancer biopathologic features, such as estrogen and progesterone receptor expression, tumor stage, vascular invasion, or proliferation index.
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miR-21 is up-regulated in the majority of human cancers profiled to date.
“The majority of these profiling studies have reported increased levels of miR-21 in most human cancers (6,(8)(9)(10).”
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- Authors
- Iorio, M. V., Ferracin, M., Liu, C.-G., Veronese, A., Spizzo, R., Sabbioni, S., Magri, E., Pedriali, M., Fabbri, M., Campiglio, M., Ménard, S., Palazzo, J. P., Rosenberg, A., Musiani, P., Volinia, S., Nenci, I., Calin, G. A., Querzoli, P., Negrini, M., & Croce, C. M.
- Affiliations
- The Ohio State University (US)
- University of Ferrara (IT)
- Fondazione IRCCS Istituto Nazionale dei Tumori (IT)
- 3Molecular Targeting Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy; Departments of
- Thomas Jefferson University (US)
- 6Ce.S.I. Aging Research Center, Chieti, Italy
- ORCID
- Venue
- Cancer Research
- Published
- 2005-08-15
- Volume
- 65
- Issue
- 16
- Pages
- 7065-7070
- ISSN
- 0008-5472 (ISSN-L)
- 1538-7445
- OpenAlex
- W2135836598
- Cited by
- 4,053
- References
- 0
- Citations by year
- 2026: 47 · 2025: 48 · 2024: 83 · 2023: 118 · 2022: 123 · 2021: 137 · 2020: 156 · 2019: 181 · 2018: 216 · 2017: 233 · 2016: 232 · 2015: 276 · 2014: 298 · 2013: 338 · 2012: 317
- FWCI
- 48.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Research (publishedVersion)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
- Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
- Funders
- National Institutes of Health (4p01ca081534-16)
- National Cancer Institute (R01CA083698, P01 CA81534, P01-CA76259, P01CA076259, CA083698)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Keywords
- microRNA, breast cancer, miRNA expression profiling, miR-125b, miR-145, miR-21, miR-155, estrogen receptor, progesterone receptor, tumor stage, vascular invasion, proliferation index, aberrant gene expression
- MeSH terms
- Breast Neoplasms, Humans, Blotting, Northern, Gene Expression Regulation, Neoplastic, Gene Expression Profiling, MicroRNAs
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