The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding
Open accessAbstract
Covalent modification by small ubiquitin-related modifiers (SUMO) regulates p53 transcription activity through an undefined mechanism. Using reconstituted sumoylation components, we purified SUMO-1-conjugated p53 (Su-p53) to near homogeneity. Su-p53 exists in solution as a tetramer and interacts with p300 histone acetyltransferase as efficiently as the unmodified protein. Nevertheless, it fails to activate p53-dependent chromatin transcription because of its inability to bind DNA. With sequential modification assays, we found that sumoylation of p53 at K386 blocks subsequent acetylation by p300, whereas p300-acetylated p53 remains permissive for ensuing sumoylation at K386 and alleviates sumoylation-inhibited DNA binding. While preventing the free form of p53 from accessing its cognate sites, sumoylation fails to disengage prebound p53 from DNA. The sumoylation-deficient K386R protein, when expressed in p53-null cells, exhibits higher transcription activity and binds better to the endogenous p21 gene compared with the wild-type protein. These studies unravel a molecular mechanism underlying sumoylation-regulated p53 function and further uncover a new role of acetylation in antagonizing the inhibitory effect of sumoylation on p53 binding to DNA.
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“Competition between Mdm2 and other proteins for modification of these sites may therefore contribute to p53 function [127].”
From The p53 orchestra: Mdm2 and Mdmx set the tone (2010) · cites this paper as [127]
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Kumar, J., Uppulapu, S. K., Kumari, S., Sharma, K., Paradee, W. J., Yadav, R. P., Kumar, V., & Kumar, S. (2025) · eLife · doi:10.7554/elife.106344.1
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Metadata
- Authors
- Wu, S., & Chiang, C.
- Affiliations
- Harold C. Simmons Comprehensive Cancer Center (US)
- Corresponding author
- Chiang, Cheng‐Ming
- Venue
- The EMBO Journal
- Published
- 2009-04-02
- Volume
- 28
- Issue
- 9
- Pages
- 1246-1259
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC2683057
- OpenAlex
- W2073590048
- Cited by
- 125
- References
- 68
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 5 · 2023: 1 · 2022: 8 · 2021: 12 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 6 · 2015: 3 · 2014: 7 · 2013: 12 · 2012: 12
- FWCI
- 5.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (CA103867, R01CA103867, R01CA124760, CA124760)
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- p53, SUMOylation, acetylation, SUMO1, P300, histone acetyltransferase, p53 DNA binding, chromatin transcription, post-translational modification, p21 gene, p53 tetramer, transcriptional activation
- MeSH terms
- p300-CBP-Associated Factor, Acetylation, Chromatin, Chromatography, Gel, DNA, Humans, Protein Binding, Transcription, Genetic, Tumor Suppressor Protein p53, Reverse Transcriptase Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay, Small Ubiquitin-Related Modifier Proteins, Cell Line, Tumor, Chromatin Immunoprecipitation, p300-CBP Transcription Factors
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