The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy
Open accessAbstract
During mammalian development, a subpopulation of endothelial cells in the cardinal vein (CV) expresses lymphatic-specific genes and subsequently develops into the first lymphatic structures, collectively termed as lymph sacs. Budding, sprouting and ballooning of lymphatic endothelial cells (LECs) have been proposed to underlie the emergence of LECs from the CV, but the exact mechanisms of lymph vessel formation remain poorly understood. Applying selective plane illumination-based ultramicroscopy to entire wholemount-immunostained mouse embryos, we visualized the complete developing vascular system with cellular resolution. Here, we report emergence of the earliest detectable LECs as strings of loosely connected cells between the CV and superficial venous plexus. Subsequent aggregation of LECs resulted in formation of two distinct, previously unidentified lymphatic structures, the dorsal peripheral longitudinal lymphatic vessel (PLLV) and the ventral primordial thoracic duct (pTD), which at later stages formed a direct contact with the CV. Providing new insights into their function, we found vascular endothelial growth factor C (VEGF-C) and the matrix component CCBE1 indispensable for LEC budding and migration. Altogether, we present a significantly more detailed view and novel model of early lymphatic development.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Prox1 Function Is Required for the Development of the Murine Lymphatic System
Wigle, J. T., & Oliver, G. (1999) · Cell · doi:10.1016/s0092-8674(00)81511-1 · Cited by 1,579
Lymphangiogenesis: Molecular Mechanisms and Future Promise
Tammela, T., & Alitalo, K. (2010) · Cell · doi:10.1016/j.cell.2010.01.045 · Cited by 1,441
Self-citationKaipainen, A., Korhonen, J., Mustonen, T., van Hinsbergh, V. W., Fang, G. H., Dumont, D., Breitman, M., & Alitalo, K. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.8.3566 · Cited by 1,423
Self-citationKarkkainen, M. J., Haiko, P., Sainio, K., Partanen, J., Taipale, J., Petrova, T. V., Jeltsch, M., Jackson, D. G., Talikka, M., Rauvala, H., et al. (2004) · Nature Immunology · doi:10.1038/ni1013 · Cited by 1,421
Self-citationAn essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype
Wigle, J. T., Harvey, N., Detmar, M., Lagutina, I., Grosveld, G., Gunn, M. D., Jackson, D. G., & Oliver, G. (2002) · The EMBO Journal · doi:10.1093/emboj/21.7.1505 · Cited by 943
Cardiovascular Failure in Mouse Embryos Deficient in VEGF Receptor-3
Dumont, D. J., Jussila, L., Taipale, J., Lymboussaki, A., Mustonen, T., Pajusola, K., Breitman, M., & Alitalo, K. (1998) · Science · doi:10.1126/science.282.5390.946 · Cited by 874
Self-citationBlocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation
Tammela, T., Zarkada, G., Wallgard, E., Murtomäki, A., Suchting, S., Wirzenius, M., Waltari, M., Hellström, M., Schomber, T., Peltonen, R., et al. (2008) · Nature · doi:10.1038/nature07083 · Cited by 813
Self-citationT1 /podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema
Schacht, V. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg342 · Cited by 653
Petrova, T. V., Mäkinen, T., Mäkelä, T. P., Saarela, J., Virtanen, I., Ferrell, R. E., Finegold, D. N., Kerjaschki, D., Ylä‐Herttuala, S., & Alitalo, K. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf470 · Cited by 632
Self-citationLineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature
Srinivasan, R. S., Dillard, M. E., Lagutin, O. V., Lin, F.-J., Tsai, S., Tsai, M.-J., Samokhvalov, I. M., & Oliver, G. (2007) · Genes & Development · doi:10.1101/gad.1588407 · Cited by 579
Cited by
Vascular heterogeneity and specialization in development and disease
Potente, M., & Mäkinen, T. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.36 · Cited by 653
Cardiac lymphatics are heterogeneous in origin and respond to injury
Klotz, L., Norman, S., Vieira, J. M., Masters, M., Rohling, M., Dubé, K. N., Bollini, S., Matsuzaki, F., Carr, C. A., & Riley, P. R. (2015) · Nature · doi:10.1038/nature14483 · Cited by 506
Development and plasticity of meningeal lymphatic vessels
Antila, S., Karaman, S., Nurmi, H., Airavaara, M., Voutilainen, M. H., Mathivet, T., Chilov, D., Li, Z., Koppinen, T., Park, J.-H., et al. (2017) · Journal of Experimental Medicine · doi:10.1084/jem.20170391 · Cited by 466
Self-citationLymphatic Vessel Network Structure and Physiology
Breslin, J. W., Yang, Y., Scallan, J. P., Sweat, R. S., Adderley, S. P., & Murfee, W. L. (2018) · Comprehensive Physiology · doi:10.1002/cphy.c180015 · Cited by 341
The role of fatty acid β-oxidation in lymphangiogenesis
Wong, B. W., Wang, X., Zecchin, A., Thienpont, B., Cornelissen, I., Kalucka, J., García-Caballero, M., Missiaen, R., Huang, H., Brüning, U., et al. (2017) · Nature · doi:10.1038/nature21028 · Cited by 336
Self-citation
Metadata
- Authors
- Hägerling, R., Pollmann, C., Andreas, M., Schmidt, C., Nurmi, H., Adams, R. H., Alitalo, K., Andresen, V., Schulte-Merker, S., & Kiefer, F.
- Venue
- The EMBO Journal
- Published
- 2013-01-08
- Volume
- 32
- Issue
- 5
- Pages
- 629-644
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- OpenAlex
- W2160167323
- Cited by
- 288
- References
- 44
- FWCI
- 17.71
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. https://app.cochat.ai/papers/10.1038/emboj.2012.340
- The paper
- Hägerling, R., Pollmann, C., Andreas, M., Schmidt, C., Nurmi, H., Adams, R. H., Alitalo, K., Andresen, V., Schulte-Merker, S., & Kiefer, F. (2013). A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. The EMBO Journal. https://doi.org/10.1038/emboj.2012.340
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
