Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E‐cadherin to disrupt intercellular adhesion

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Items 101–200 of 295

1. Function of serine protease HtrA in the lifecycle of the foodborne pathogen Campylobacter jejuni
   Manja Boehm, Daniel Simson, Ulrike Escher, Anna-Maria Schmidt, Stefan Bereswill, Nicole Tegtmeyer, Steffen Backert, Markus M. Heimesaat · 2018 · European Journal of Microbiology and Immunology
2. Exploiting the Gastric Epithelial Barrier: Helicobacter pylori’s Attack on Tight and Adherens Junctions
   Steffen Backert, Thomas P. Schmidt, Aileen Harrer, Silja Weßler · 2017 · Current topics in microbiology and immunology
3. Delivery of Toxins and Effectors by Bacterial Membrane Vesicles
   Adrian Macion, Agnieszka Wyszyńska, Renata Alicja Godlewska · 2021 · Toxins
4. HtrA, a Temperature- and Stationary Phase-Activated Protease Involved in Maturation of a Key Microbial Virulence Determinant, Facilitates Borrelia burgdorferi Infection in Mammalian Hosts
   Meiping Ye, Kavita Sharma, Meghna Thakur, Alexis A. Smith, Özlem Büyüktanır, Xuwu Xiang, Xiuli Yang, Kamoltip Promnares, Yongliang Lou, X. Frank Yang et al. · 2016 · Infection and Immunity
5. Pathogenicity and virulence of Helicobacter pylori : A paradigm of chronic infection
   Marguerite Clyne, Tadhg Ó Cróinı́n · 2024 · Virulence
6. Structural and functional aspects of theHelicobacter pylorisecretome
   Giuseppe Zanotti · 2014 · World Journal of Gastroenterology
7. Digestive Inflammation: Role of Proteolytic Dysregulation
   Vincent Mariaule, Aïcha Kriaa, Souha Soussou, Soufien Rhimi, Houda Boudaya, Juan L. Hernandez, Emmanuelle Maguin, Adam Lesner, Moez Rhimi · 2021 · International Journal of Molecular Sciences
8. Helicobacter pylori bacteria alter the p53 stress response via Erk-HDM2 pathway
   Vikas Bhardwaj, Jennifer M. Noto, Jinxiong Wei, Claudia D. Andl, Wael M. El-Rifai, Richard M. Peek, Alexander I. Zaika · 2015 · Oncotarget
9. The Family Helicobacteraceae
   Hazel M. Mitchell, Gifone Aguiar Rocha, Nadeem Omar Kaakoush, Jani O’Rourke, Dulciene M. M. Queiroz · 2014 · Springer eBooks
10. Helicobacter pyloridefense against oxidative attack
    Andrew William Stent, Alison L. Every, PHILIP R. N. SUTTON · 2011 · American Journal of Physiology-Gastrointestinal and Liver Physiology
11. The impact of serine protease HtrA in apoptosis, intestinal immune responses and extra-intestinal histopathology during Campylobacter jejuni infection of infant mice
    Markus M. Heimesaat, André Fischer, Marie E. Alutis, Ursula Grundmann, Manja Boehm, Nicole Tegtmeyer, Ulf Berthold Göbel, Anja Andrea Kühl, Stefan Bereswill, Steffen Backert · 2014 · Gut Pathogens
12. The Inhibitory Effect of IFN-γ on Protease HTRA1 Expression in Rheumatoid Arthritis
    Yuzhu Hou, Haijiang Lin, Linnan Zhu, Zhaoting Liu, Fanlei Hu, Jianfeng Shi, Tao Yang, Xiaoyun Shi, Huifang Guo, Xiaotian Tan et al. · 2014 · The Journal of Immunology
13. Establishment of serine protease htrA mutants in Helicobacter pylori is associated with secA mutations
    Anna Magdalena Zawilak-Pawlik, Urszula Zarzecka, Dorota Żyła-Uklejewicz, Jakub Lach, Dominik Strapagiel, Nicole Tegtmeyer, Manja Böhm, Steffen Backert, Joanna Skórko‐Glonek · 2019 · Scientific Reports
14. Chaperone activity of serine protease HtrA of Helicobacter pylori as a crucial survival factor under stress conditions
    Urszula Zarzecka, Aileen Harrer, Anna Magdalena Zawilak-Pawlik, Joanna Skórko‐Glonek, Steffen Backert · 2019 · Cell Communication and Signaling
15. Microevolution of Virulence-Related Genes in Helicobacter pylori Familial Infection
    Yoshikazu Furuta, Mutsuko Konno, Takako Osaki, Hideo Yonezawa, Taichiro Ishige, Misaki Imai, Yuh Shiwa, Mari Shibata-Hatta, Yu Kanesaki, Hirofumi Yoshikawa et al. · 2015 · PLoS ONE
16. Helicobacter pylori-induced posttranscriptional regulation of H-K-ATPase α-subunit gene expression by miRNA
    Yongmei Zhang, Jennifer M. Noto, Charles E. Hammond, Jeremy L. Barth, William Scott Argraves, Steffen Backert, Richard M. Peek, Adam J. Smolka · 2014 · American Journal of Physiology-Gastrointestinal and Liver Physiology
17. Calcium binding protects E-cadherin from cleavage by Helicobacter pylori HtrA
    Thomas P. Schmidt, Camilla Goetz, Markus Huemer, Gisbert Schneider, Silja Weßler · 2016 · Gut Pathogens
18. Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains
    Judith Lind, Steffen Backert, Rebecca Hoffmann, Jutta Eichler, Yoshio Yamaoka, Guillermo I. Perez-Perez, Javier Torres, Heinrich Sticht, Nicole Tegtmeyer · 2016 · BMC Microbiology
19. Structure‐Based Pharmacophores for Virtual Screening
    Martin Löwer, Ewgenij Proschak · 2011 · Molecular Informatics
20. Afadin Downregulation by Helicobacter pylori Induces Epithelial to Mesenchymal Transition in Gastric Cells
    Miguel S. Marques, Joana Melo, Bruno Cavadas, Nuno M. Mendes, Luı́sa Pereira, Fátima Carneiro, Céu Figueiredo, Marina Leite · 2018 · Frontiers in Microbiology
21. Activity and Functional Importance of Helicobacter pylori Virulence Factors
    Dionyssios N. Sgouras, Nicole Tegtmeyer, Silja Weßler · 2019 · Advances in experimental medicine and biology
22. A novel FRET peptide assay reveals efficient Helicobacter pylori HtrA inhibition through zinc and copper binding
    Sabine Bernegger, Cyrill Brunner, Matej Vizovišek, Marko Fonovič, Gaetano Cuciniello, Flavia Giordano, Vesna Stanojlović, Mirosław Jarząb, Philip C. Simister, Stephan M. Feller et al. · 2020 · Scientific Reports
23. Proteolysis in Helicobacter pylori-Induced Gastric Cancer
    Gernot Posselt, Jean E. Crabtree, Silja Weßler · 2017 · Toxins
24. Crohn’s disease proteolytic microbiota enhances inflammation through PAR2 pathway in gnotobiotic mice
    Alba Santiago, Amber Hann, Marco Constante, Sara Rahmani, Josie Libertucci, Kyle J. Jackson, Gaston H. Rueda, Laura Rossi, Rithwik Ramachandran, Wolfram Ruf et al. · 2023 · Gut Microbes
25. Electron Microscopic, Genetic and Protein Expression Analyses of Helicobacter acinonychis Strains from a Bengal Tiger
    Nicole Tegtmeyer, Francisco Rivas-Traverso, Manfred Rohde, Omar A. Oyarzábal, Norbert Lehn, Wulf Schneider‐Brachert, Richard L. Ferrero, James G. Fox, Douglas E. Berg, Steffen Backert · 2013 · PLoS ONE
26. Amino-Terminal Processing of Helicobacter pylori Serine Protease HtrA: Role in Oligomerization and Activity Regulation
    Nicole Albrecht, Nicole Tegtmeyer, Heinrich Sticht, Joanna Skórko‐Glonek, Steffen Backert · 2018 · Frontiers in Microbiology
27. Flavodoxins as Novel Therapeutic Targets against Helicobacter pylori and Other Gastric Pathogens
    Sandra Salillas, Javier Sancho · 2020 · International Journal of Molecular Sciences
28. HtrA family proteases of bacterial pathogens: pros and cons for their therapeutic use
    Ruo‐Yi Xue, Chang Liu, Qing-Tao Xiao, Si Sun, Quanming Zou, Haibo Li · 2021 · Clinical Microbiology and Infection
29. Infiltration to infection: key virulence players of Helicobacter pylori pathogenicity
    Arghyadeep Bhattacharjee, Om Saswat Sahoo, Ahana Sarkar, Saurabh Bhattacharya, Rukhsana Chowdhury, Samarjit Kar, Oindrilla Mukherjee · 2024 · Infection
30. Inhibiting Helicobacter pylori HtrA protease by addressing a computationally predicted allosteric ligand binding site
    Anna Maria Perna, Felix Reisen, Thomas P. Schmidt, Tim Geppert, Max Pillong, Martin Weisel, Benjamin Hoy, Philip C. Simister, Stephan M. Feller, Silja Weßler et al. · 2014 · Chemical Science
31. Protease Activity of Campylobacter jejuni HtrA Modulates Distinct Intestinal and Systemic Immune Responses in Infected Secondary Abiotic IL-10 Deficient Mice
    Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Manja Boehm, Steffen Backert, Stefan Bereswill, Markus M. Heimesaat · 2019 · Frontiers in Cellular and Infection Microbiology
32. The stability and activity of recombinant Helicobacter pylori Htr A under stress conditions
    Benjamin Hoy, Hans Brandstetter, Silja Weßler · 2012 · Journal of Basic Microbiology
33. Cancer invasion and anaerobic bacteria: new insights into mechanisms
    Rachel Hurst, Daniel S. Brewer, Abraham Gihawi, John R. Wain, Colin S. Cooper · 2024 · Journal of Medical Microbiology
34. B orrelia burgdorferi HtrA may promote dissemination and irritation
    Frank C. Gherardini · 2013 · Molecular Microbiology
35. Extracellular secretion of protease HtrA fromCampylobacter jejuniis highly efficient and independent of its protease activity and flagellum
    Manja Boehm, Ingrid Haenel, Benjamin Hoy, Lone Brøndsted, Todd G. Smith, Timothy R. Hoover, Silja Weßler, Nicole Tegtmeyer · 2013 · European Journal of Microbiology and Immunology
36. Morphological changes in human gastric epithelial cells induced by nuclear targeting of Helicobacter pylori urease subunit A
    Jung Hwa Lee, So Hyun Jun, Jung‐Min Kim, Seung Chul Baik, Je Chul Lee · 2015 · The Journal of Microbiology
37. Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis
    Weronika Gonciarz, Agnieszka Krupa, Magdalena Chmiela · 2020 · International Journal of Molecular Sciences
38. Genetic populations and virulence factors of Helicobacter pylori
    Evariste Tshibangu‐Kabamba, Vo Phuoc Tuan, Yoshio Yamaoka · 2018 · Infection Genetics and Evolution
39. Design of a Helicobacter pylori multi-epitope vaccine based on immunoinformatics
    Man Cui, Xiaohui Ji, Fengtao Guan, Guimin Su, Lin Du · 2024 · Frontiers in Immunology
40. The role of microbiota in the development and treatment of gastric cancer
    Yiwen Wang, Wenjie Han, Na Wang, Mengzhen Han, Meng Ban, Jianying Dai, Yuesheng Dong, Tao Sun, Junnan Xu · 2023 · Frontiers in Oncology
41. Growth phase-dependent composition of the Helicobacter pylori exoproteome
    Christina A. Snider, Bradley J. Voss, William Hayes McDonald, Timothy L. Cover · 2015 · Journal of Proteomics
42. The Periplasmic PDZ Domain–Containing Protein Prc Modulates Full Virulence, Envelops Stress Responses, and Directly Interacts with Dipeptidyl Peptidase ofXanthomonas oryzaepv.oryzae
    Chao‐Ying Deng, Aihua Deng, Shutao Sun, Li Wang, Jie Wu, Yao Wu, Xiaoying Chen, Rongxiang Fang, Tingyi Wen, Wei Qian · 2013 · Molecular Plant-Microbe Interactions
43. Helicobacter pylori‐induced alteration of epithelial cell signaling and polarity: A possible mechanism of gastric carcinoma etiology and disparity
    Mahasin A. Osman, George S. Bloom, Emmanuel Ayitey Tagoe · 2013 · Cytoskeleton
44. The salt‐sensitive structure and zinc inhibition of B orrelia burgdorferi protease BbHtrA
    Theresa M. Russell, Xiaoling Tang, Jason M. Goldstein, Dennis Bagarozzi, Barbara J. B. Johnson · 2015 · Molecular Microbiology
45. H. pylori isolates with amino acid sequence polymorphisms as presence of both HtrA-L171 & CagL-Y58/E59 increase the risk of gastric cancer
    Yi-Chun Yeh, Hsin‐Yu Kuo, Wei-Lun Chang, Hsiao‐Bai Yang, Cheng‐Chan Lu, Hsiu‐Chi Cheng, Ming‐Shiang Wu, Bor‐Shyang Sheu · 2019 · Journal of Biomedical Science
46. Interference of LPS H. pylori with IL-33-Driven Regeneration of Caviae porcellus Primary Gastric Epithelial Cells and Fibroblasts
    Weronika Gonciarz, Agnieszka Krupa, Anthony P. Moran, Agata Tomaszewska, Magdalena Chmiela · 2021 · Cells
47. Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells
    Sabine Bernegger, Robert Vidmar, Marko Fonovič, Gernot Posselt, Boris Turk, Silja Weßler · 2021 · Cell Communication and Signaling
48. Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis
    Frédéric Samazan, Bachra Rokbi, Delphine Seguin, Fabienne Telles, Valérie Gautier, Gilbert Richarme, Didier Chevret, Paloma F. Varela, Christophe Velours, Isabelle Poquet · 2015 · Microbial Cell Factories
49. The protease inhibitor JO146 demonstrates a critical role for CtHtrA for Chlamydia trachomatis reversion from penicillin persistence
    Vanissa A. Ong, J. Wallis Marsh, Amba Lawrence, JOHN A. B. ALLAN, Peter Timms, Wilhelmina May Huston · 2013 · Frontiers in Cellular and Infection Microbiology
50. The LA Loop as an Important Regulatory Element of the HtrA (DegP) Protease from Escherichia coli
    Donata Figaj, Artur Giełdoń, Agnieszka Polit, Anna Sobiecka-Szkatuła, Tomasz Koper, Milena Denkiewicz-Kruk, Bogdan Banecki, Adam Lesner, Jerzy Ciarkowski, Barbara Lipińska et al. · 2014 · Journal of Biological Chemistry
51. The unique trimeric assembly of the virulence factor HtrA from Helicobacter pylori occurs via N-terminal domain swapping
    Zhemin Zhang, Qi Huang, Xuan Tao, Guobing Song, Peng Zheng, Hongyan Li, Hongzhe Sun, Wei Ting Xia · 2019 · Journal of Biological Chemistry
52. Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni
    Renata Alicja Godlewska, Joanna Klim, Janusz Dȩbski, Agnieszka Wyszyńska, Anna M. Łasica · 2019 · Polish Journal of Microbiology
53. <i>Helicobacter pylori</i> PqqE is a new virulence factor that cleaves junctional adhesion molecule A and disrupts gastric epithelial integrity
    Marques, Miguel S., Costa, Ana C., Osório, Hugo, Pinto, Marta L., Relvas, Sandra, Dinis-Ribeiro, Mário, Carneiro, Fátima, Leite, Marina, Figueiredo, Ceu · 2021 · Gut Microbes
54. Human–gut bacterial protein–protein interactions: understudied but impactful to human health
    Diana Balint, Ilana Brito · 2023 · Trends in Microbiology
55. Digestion of epithelial tight junction proteins by the commensalClostridium perfringens
    Mihaela Pruteanu, Fergus Shanahan · 2013 · American Journal of Physiology-Gastrointestinal and Liver Physiology
56. A role for altered phagosome maturation in the long-term persistence ofHelicobacter pyloriinfection
    Glenn N. Borlace, Stacey J. Keep, Mark J. Prodoehl, Hilary F. Jones, Ross N. Butler, Doug A. Brooks · 2012 · American Journal of Physiology-Gastrointestinal and Liver Physiology
57. B orrelia burgdorferi HtrA : evidence for twofold proteolysis of outer membrane protein p66
    James L. Coleman, Alvaro M. Toledo, Jorge L. Benach · 2015 · Molecular Microbiology
58. Draft Genome Sequences of Six Enterohepatic Helicobacter Species Isolated from Humans and One from Rhesus Macaques
    Zeli Shen, Alexander Sheh, Sarah Young, Amr Abouelliel, Doyle V. Ward, Ashlee M. Earl, James G. Fox · 2014 · Genome Announcements
59. Extracellular proteases from microbial plant pathogens as virulence factors
    Jessica Lee Erickson, Mariana Schuster · 2024 · Current Opinion in Plant Biology
60. Targeting of the apical junctional complex by bacterial pathogens
    Philippe Huber · 2020 · Biochimica et Biophysica Acta (BBA) - Biomembranes
61. Association Between Helicobacter pylori and Gastric Carcinoma
    Jaskamal Padda, Khizer Khalid, Ayden Charlene Cooper, Gutteridge Jean-Charles · 2021 · Cureus
62. Emerging therapeutic targets for gastric cancer from a host-Helicobacter pylori interaction perspective
    Esmat Abdi, Saeid Latifi‐Navid, Fatemeh Abedi Sarvestani, Mohammad Hassan Esmailnejad · 2021 · Expert Opinion on Therapeutic Targets
63. Under the Radar: Strategies Used by Helicobacter pylori to Evade Host Responses
    Akriti Prashar, Mariana Capurro, Nicola L. Jones · 2021 · Annual Review of Physiology
64. Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view
    Yingjie Song, Yitao Ke, Mei Kang, Rui Bao · 2021 · Computational and Structural Biotechnology Journal
65. The immunopathogenesis of Helicobacter pylori-induced gastric cancer: a narrative review
    Maryam Shirani, Saeedeh Shariati, Monireh Bazdar, Faezeh Sojoudi Ghamnak, Melika Moradi, Reyhaneh Shams Khozani, Elahe Taki, Zahra Arabsorkhi, Mohsen Heidary, Dorsa Bahrami Eskandari · 2024 · Frontiers in Microbiology
66. Genome-Wide Association Analysis of Avian Resistance to Campylobacter jejuni Colonization Identifies Risk Locus Spanning the CDH13 Gene
    Sarah Connell, Kieran G. Meade, Brenda J Allan, Andrew T. Lloyd, Tim Downing, Cliona O’Farrelly, Daniel G. Bradley · 2013 · G3 Genes Genomes Genetics
67. Role of caspase-3/E-cadherin in helicobacter pylori-induced apoptosis of gastric epithelial cells
    Yongmei Yang, Jie Du, Fen Liu, Xiaoyan Wang, Xiaohui Li, Yuan‐Jian Li · 2017 · Oncotarget
68. T Cell Cytokines Impact Epithelial Cell Responses during Helicobacter pylori Infection
    Holly M. Scott Algood · 2020 · The Journal of Immunology
69. Disassembling a cancer puzzle: Cell junctions and plasma membrane as targets for anticancer therapy
    Olga N. Shilova, Evgeny S. Shilov, André Lieber, Sergey Mikhailovich Deyev · 2018 · Journal of Controlled Release
70. Distinct Contribution of the HtrA Protease and PDZ Domains to Its Function in Stress Resilience and Virulence of Bacillus anthracis
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71. Importance of two PDZ domains for the proteolytic and chaperone activities ofHelicobacter pyloriserine protease HtrA
    Urszula Zarzecka, Dorota Matkowska, Steffen Backert, Joanna Skórko‐Glonek · 2020 · Cellular Microbiology
72. Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA
    Urszula Zarzecka, Alessandro Grinzato, Eaazhisai Kandiah, Dominik Cysewski, Paola Berto, Joanna Skórko‐Glonek, Giuseppe Zanotti, Steffen Backert · 2020 · Gut Microbes
73. Trimer stability of Helicobacter pylori HtrA is regulated by a natural mutation in the protease domain
    Urszula Zarzecka, Nicole Tegtmeyer, Heinrich Sticht, Steffen Backert · 2023 · Medical Microbiology and Immunology
74. Identification of Potential Drug Targets in Helicobacter pylori Strain HPAG1 by in silico Genome Analysis
    Nageswara Rao Reddy Neelapu, Naresh V. R. Mutha, Srinivas Akula · 2015 · Infectious Disorders - Drug Targets
75. Effect of environmental salt concentration on the Helicobacter pylori exoproteome
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76. Cortactin: A Major Cellular Target of the Gastric Carcinogen Helicobacter pylori
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77. Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells
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78. Bacterial membrane vesicles in inflammatory bowel disease
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79. Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
    Luhua Zhang, Yiping Wen, Ying Li, Xingliang Wei, Xuefeng Yan, Xintian Wen, Rui Jin Wu, Xiaobo Huang, Yong Huang, Qigui Yan et al. · 2014 · Proteome Science
80. Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma
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81. The Active Site Residue V266 of Chlamydial HtrA Is Critical for Substrate Binding during both in vitro and in vivo Conditions
    Sarina Gloeckl, Joel D. A. Tyndall, Scott H. Stansfield, Peter Timms, Wilhelmina May Huston · 2012 · Microbial Physiology
82. Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni
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83. Pneumococcal Extracellular Serine Proteases: Molecular Analysis and Impact on Colonization and Disease
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84. Extracellular Pneumococcal Serine Proteases Affect Nasopharyngeal Colonization
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85. Insights into the Cyanobacterial Deg/HtrA Proteases
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86. Production and purification of outer membrane vesicles encapsulating green fluorescent protein from Escherichia coli: a step towards scalable OMV technologies
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87. Immune evasion by proteolytic shedding of natural killer group 2, member D ligands in Helicobacter pylori infection
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88. Relationship between histopathological status of the Helicobacter pylori infected patients and proteases of H. pylori in isolates carrying diverse virulence genotypes
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89. The Role of Bacterial Proteases in Microbe and Host-microbe Interactions
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90. Proteolytic Landscapes in Gastric Pathology and Cancerogenesis
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91. Pathogenomics of Helicobacter pylori
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92. Helicobacter pyloriinfection impairs chaperone-assisted maturation of Na-K-ATPase in gastric epithelium
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93. Gastric Epithelial Barrier Disruption, Inflammation and Oncogenic Signal Transduction by Helicobacter pylori
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94. Candidate Antigens and the Development of Helicobacter pylori Vaccines
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95. Cortactin Is Required for Efficient FAK, Src and Abl Tyrosine Kinase Activation and Phosphorylation of Helicobacter pylori CagA
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96. How many protein molecules are secreted by single Helicobacter pylori cells: Quantification of serine protease HtrA
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97. From Virtual Screening to Bioactive Compounds by Visualizing and Clustering of Chemical Space
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98. MECHANISMS OF INTERRACTION OF HELICOBACTER PYLORI WITH EPITHELIUM OF GASTRIC MUCOSA. I. PATHOGENIC FACTORS PROMOTING SUCCESSFUL COLONIZATION
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99. The proteolytic activity of Listeria monocytogenes HtrA
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100. Proteolytic activation of Chlamydia trachomatis HTRA is mediated by PDZ1 domain interactions with protease domain loops L3 and LC and beta strand β5
     J. Wallis Marsh, William B. Lott, Joel D. A. Tyndall, Wilhelmina May Huston · 2013 · Cellular & Molecular Biology Letters