The DOI matches its Crossref record; 29 of the 31 references it cites were found in a registry or an index.
Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts.
Open accessAbstract
Mitochondrial biogenesis is known to accompany adipogenesis to complement ATP and acetyl-CoA required for lipogenesis. Here, we demonstrated that mitochondrial proteins such as ATP synthase alpha and beta, and cytochrome c were highly expressed during the 3T3-L1 differentiation into adipocytes. Fully-differentiated adipocytes showed a significant increase of mitochondria under electron microscopy. Analysis by immunofluorescence, cellular fractionation, and surface biotinylation demonstrated the elevated levels of ATP synthase complex found not only in the mitochondria but also on the cell surface (particularly lipid rafts) of adipocytes. High rate of ATP (more than 30 microM) synthesis from the added ADP and P(i) in the adipocyte media suggests the involvement of the surface ATP synthase complex for the extracellular ATP synthesis. In addition, this ATP synthesis was significantly inhibited in the presence of oligomycin, an ATP synthase inhibitor, and carbonyl cyanide m-chlorophenylhydrazone (CCCP), an ATP synthase uncoupler. Decrease of extracellular ATP synthesis in acidic but not in basic media further indicates that the surface ATP synthase may also be regulated by proton gradient through the plasma membrane.
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“However, mitochondrial density increases dramatically, and mitochondrial remodeling occurs during white adipocyte differentiation (156 -158), suggesting that mitochondrial functions are required to support the multiple biological roles of mature white adipocytes.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [157]
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Cites
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Angiostatin binds ATP synthase on the surface of human endothelial cells
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2 of the 31 references couldn’t be matched to a known paper.
Research works printed but not linked: 6
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Items 101–128 of 128
The ATP synthase subunit β (ATP5B) is an entry factor for the hepatitis E virusPreprint
Ahmed, Z., Holla, P., Ahmad, I., & Jameel, S. (2016) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/060434 · Cited by 12
“This could be due to binding of the HEV-LPs to the ectopic ATP synthase complex, which resides in these membrane fractions (Kim et al., 2004).”
BackgroundUnveiling the dynamics of acetylation and phosphorylation in SGBS and 3T3-L1 adipogenesis
Aldehoff, A. S., Karkossa, I., Goerdeler, C., Krieg, L., Schor, J., Engelmann, B., Wabitsch, M., Landgraf, K., Hackermüller, J., Körner, A., et al. (2024) · iScience · doi:10.1016/j.isci.2024.109711 · Cited by 8
“42,43 Despite the finding that downregulation of several HDACs is important for adipogenic differentiation, 44 HDAC11 deficiency was found to reduce lipid accumulation and PPAR g levels, 45 thus an increasing abundance of HDAC11 and PPAR g during adipogenesis as seen in our SGBS transcript data…”
Kim, B.-E., Lee, J., Kim, H., Kwon, O., Jeong, S., & Kim, I. (2004) · Experimental & Molecular Medicine · doi:10.1038/emm.2004.56 · Cited by 8
Zhu, B., Feng, Z., Guo, Y., Zhang, T., Mai, A., Kang, Z., Weijen, T., Wang, D., Yin, D., Zhu, D., et al. (2020) · Cell Communication and Signaling · doi:10.1186/s12964-020-0515-3 · Cited by 8
Application of displacement chromatography for the analysis of a lipid raft proteome
Trusch, M., Böhlick, A., Hildebrand, D., Lichtner, B., Bertsch, A., Kohlbacher, O., Bachmann, S., & Schlüter, H. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.11.035 · Cited by 7
Gallinat, A., & Badimón, L. (2022) · Scientific Reports · doi:10.1038/s41598-022-16998-3 · Cited by 7
P2Y receptors in atherosclerosis: from lipid metabolism to vascular functions
Martinez, L. O., Cabou, C., Pons, V., & Malaval, C. (2012) · Wiley Interdisciplinary Reviews Membrane Transport and Signalling · doi:10.1002/wmts.66 · Cited by 4
Gao, J., Li, J., Feng, C., Hu, Z., Liu, W., Liang, S., & Yin, D. (2012) · Electrophoresis · doi:10.1002/elps.201200243 · Cited by 3
Ahmad, Z., Dadi, P. K., Elord, J., & Kady, I. O. (2012) · Advances in Biological Chemistry · doi:10.4236/abc.2012.22019 · Cited by 3
“Other reports have shown that ATP synthase is expressed in the extracellular surface of endothelial cells in some tumor tissues; however, its function with regard to cancer development is still not clear [12-14].”
BackgroundNann, D., Berg, C. P., Preuß, B. E., & Klein, R. (2012) · BMC Gastroenterology · doi:10.1186/1471-230x-12-152 · Cited by 3
“The induction of antibodies to F1F0-ATPase remains still obscure; however, it has been shown that the F1F0ATPase is expressed on the cell surface of endothelial cells, adipocytes, hepatocytes, and tumor cells, the F1domain facing outside [26-30].”
An insight into anti-adipogenic properties of an Oroxylum indicum (L.) Kurz extract
Hengpratom, T., Lowe, G. M., & Eumkeb, G. (2020) · BMC Complementary Medicine and Therapies · doi:10.1186/s12906-020-03111-2 · Cited by 2
Proteomic Analysis of Bovine Longissimus Muscle Satellite Cells during Adipogenic Differentiation
Rajesh, R. V., Park, M.-R., Heo, K., Yoon, D., Kim, T.-H., & Lee, H. J. (2011) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.2011.10345 · Cited by 2
“Also, lipid biosynthesis requires mitochondrial enzymes as they are involved in the formation of acetyl-CoA, ATP and NADPH, that are essential for lipid biosynthesis and ATP synthase is known for oxidative respiration to generate NADPH and ATP (Kim et al., 2004).”
BackgroundInfluentialHepatic and Renal HDL Receptors
Martinez, L. O., Perret, B., Barbaras, R., Tercé, F., & Collet, X. (2007) · Wiley eBooks · doi:10.1002/9783527625178.ch12 · Cited by 3
Role of cell surface H+-ATP synthase on adipocyte differentiation
Kita, T., Nishida, H., Niimi, S., Shibata, H., Yamazaki, T., & Arakaki, N. (2010) · Inflammation and Regeneration · doi:10.2492/inflammregen.30.425 · Cited by 1
La F1F0 ATP sintasa: un complejo proteico con gran versatilidad estructural y funcional
Domínguez‐Ramírez, L., & de Gómez‐Puyou, M. T. (2005) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · Cited by 1
Type 2 Diabetes and Mitochondria
Choo, H. J., & Ko, Y. (2006) · Journal of Korean Endocrine Society · doi:10.3803/jkes.2006.21.4.266 · Cited by 1
“갈색지방 에 비해 백색지방은 미토콘드리아의 수가 매우 적어 그 역 할이 간과되어 왔으나, 최근에 3T3-L1 백색지방 세포주가 분화할 때 미토콘드리아가 집중적으로 증가한다는 보고가 있다[24,35].”
Pinheiro, C. S., Staub, O., Mulvenna, J. P., Loukas, A. C., Jones, M. K., & Rabelo, É. M. L. (2009) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2009.01.009 · Cited by 1
Stabilization of the cortical cytoskeleton by the lipid raft-associated protein cap23
Wacha, S. (2006) · edoc (University of Basel) · doi:10.5451/unibas-004088461
Effects of palatable diets on appetite regulation, appetite peptides and neurogenesis.
Lindqvist, A. (2006) · Lund University Publications (Lund University)
Molecular interactions underlying neuronal ceroid lipofuscinoses CLN1 and CLN5
Lyly, A. (2008) · Työväentutkimus Vuosikirja
ANIMAL MODEL AND MOLECULAR INTERACTIONS OF CLN5
Tion, A. D. A. (2009)
HIGH-DENSITY LIPOPROTEINS AND APOLIPOPROTEIN A-I WITH AORTIC ENDOTHELIAL CELLS
Ohnsorg, P. M. (2011)
Mitochondrial ATP Synthase Trafficking along Microtubules to Cell Surface Depends on KIF5B and DRP1Preprint
Chang, Y., Yang, T. T., Chen, M., Liaw, Y.-G., Yin, C.-F., Huang, T.-Y., Hou, J.-T., Hung, Y.-H., Hsu, C., Huang, H., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.10.23.352385
“…has shown that several ATP synthase subunits are present on the plasma membrane (PM) of various cells, including endothelial cells, adipocytes, keratinocytes, hepatocytes, and some types of cancer cells (Arakaki et al, 2003; Inoue et al, 2007; Kim et al, 2004; Li et al, 2017; Yonally et al, 2006).”
BackgroundZhang, D., Jiang, Y., Dong, Y., Fu, L., Zhuang, L., Wu, K., Dou, X., Xu, B., Wang, C., & Gong, J. (2023) · Avian Pathology · doi:10.1080/03079457.2023.2243842
“ious cells, such as hepatocytes, human umbilical vein endothelial cells (HUVEC), and adipocytes (Kim et al., 2004; Martinez et al., 2003; Moser et al., 1999).”
BackgroundBertomeu, J. B., Fioravanço, L. P., Ramis, T. R., Godinho, D. B., Nascimento, A. S., Lima, G. C., Furian, A. F., Oliveira, M. S., Fighera, M. R., & Royes, L. F. F. (2024) · Molecular Neurobiology · doi:10.1007/s12035-024-04613-7
“The strong relationship observed between low mitochondrial ATP levels and decreased CA activity reinforces the involvement of this enzyme on oxidative phosphorylation [140, 141] and suggests that exercise protocol details have variable effects on this pathway.”
Animal model and molecular interactions of Cln5
von Schantz, C. (2009) · STM:n Hallinnonalan avoin julkaisuarkisto (Julkari)
Mécanismes de régulation de l’ecto-F1-ATPase par la mitophagie
Fried, S. (2018) · theses.fr (ABES) · doi:10.70675/f53f2ce5z6e85z479bz9bbbz68e1878cc5bc
Surface Complex V couples proton gradient across the plasma membrane to ATP production in cancerPreprint
Pelaez, D., McLaughlin, S. A., Knechtel, K., Correa, Z., & Harbour, J. W. (2026) · Research Square · doi:10.21203/rs.3.rs-10007862/v1
“This framework reconciles prior reports of extracellular ATP production 21,33,40,41 with our observation that intracellular ATP generation predominates under physiologically relevant tumor conditions.”
Metadata
- Authors
- Kim, B.-W., Choo, H.-J., Lee, J.-W., Kim, J.-H., & Ko, Y.-G.
- Affiliations
- Korea University (KR)
- ORCID
- Kim, Ji-Hyun
- Venue
- Experimental & Molecular Medicine
- Published
- 2004-10-01
- Volume
- 36
- Issue
- 5
- Pages
- 476-485
- ISSN
- 1226-3613 (ISSN-L)
- 2092-6413
- OpenAlex
- W2033234176
- Cited by
- 128
- References
- 31
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 7 · 2018: 3 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 1 · 2012: 6
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Experimental & Molecular Medicine (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- extracellular ATP, ATP synthase, adipocytes, lipid rafts, mitochondrial biogenesis, adipogenesis, 3T3-L1 cells, cytochrome c, oligomycin, CCCP, proton gradient, plasma membrane, mitochondrial protein translocation
- MeSH terms
- Adenosine Triphosphate, Animals, Cell Differentiation, Cell Membrane, Cells, Cultured, Humans, Mitochondria, Adipocytes, Membrane Microdomains, Mitochondrial Proton-Translocating ATPases, Mice
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