The DOI matches its Crossref record; 29 of the 31 references it cites were found in a registry or an index.
Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts.
Open accessAbstract
Mitochondrial biogenesis is known to accompany adipogenesis to complement ATP and acetyl-CoA required for lipogenesis. Here, we demonstrated that mitochondrial proteins such as ATP synthase alpha and beta, and cytochrome c were highly expressed during the 3T3-L1 differentiation into adipocytes. Fully-differentiated adipocytes showed a significant increase of mitochondria under electron microscopy. Analysis by immunofluorescence, cellular fractionation, and surface biotinylation demonstrated the elevated levels of ATP synthase complex found not only in the mitochondria but also on the cell surface (particularly lipid rafts) of adipocytes. High rate of ATP (more than 30 microM) synthesis from the added ADP and P(i) in the adipocyte media suggests the involvement of the surface ATP synthase complex for the extracellular ATP synthesis. In addition, this ATP synthesis was significantly inhibited in the presence of oligomycin, an ATP synthase inhibitor, and carbonyl cyanide m-chlorophenylhydrazone (CCCP), an ATP synthase uncoupler. Decrease of extracellular ATP synthesis in acidic but not in basic media further indicates that the surface ATP synthase may also be regulated by proton gradient through the plasma membrane.
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“However, mitochondrial density increases dramatically, and mitochondrial remodeling occurs during white adipocyte differentiation (156 -158), suggesting that mitochondrial functions are required to support the multiple biological roles of mature white adipocytes.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [157]
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Flagged first, then by citations
2 of the 31 references couldn’t be matched to a known paper.
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Metadata
- Authors
- Kim, B.-W., Choo, H.-J., Lee, J.-W., Kim, J.-H., & Ko, Y.-G.
- Affiliations
- Korea University (KR)
- ORCID
- Kim, Ji-Hyun
- Venue
- Experimental & Molecular Medicine
- Published
- 2004-10-01
- Volume
- 36
- Issue
- 5
- Pages
- 476-485
- ISSN
- 1226-3613 (ISSN-L)
- 2092-6413
- OpenAlex
- W2033234176
- Cited by
- 128
- References
- 31
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 7 · 2018: 3 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 1 · 2012: 6
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Experimental & Molecular Medicine (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- extracellular ATP, ATP synthase, adipocytes, lipid rafts, mitochondrial biogenesis, adipogenesis, 3T3-L1 cells, cytochrome c, oligomycin, CCCP, proton gradient, plasma membrane, mitochondrial protein translocation
- MeSH terms
- Adenosine Triphosphate, Animals, Cell Differentiation, Cell Membrane, Cells, Cultured, Humans, Mitochondria, Adipocytes, Membrane Microdomains, Mitochondrial Proton-Translocating ATPases, Mice
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