Laboratory evolution of Geobacter sulfurreducens for enhanced growth on lactate via a single-base-pair substitution in a transcriptional regulator
Open accessAbstract
The addition of organic compounds to groundwater in order to promote bioremediation may represent a new selective pressure on subsurface microorganisms. The ability of Geobacter sulfurreducens, which serves as a model for the Geobacter species that are important in various types of anaerobic groundwater bioremediation, to adapt for rapid metabolism of lactate, a common bioremediation amendment, was evaluated. Serial transfer of five parallel cultures in a medium with lactate as the sole electron donor yielded five strains that could metabolize lactate faster than the wild-type strain. Genome sequencing revealed that all five strains had non-synonymous single-nucleotide polymorphisms in the same gene, GSU0514, a putative transcriptional regulator. Introducing the single-base-pair mutation from one of the five strains into the wild-type strain conferred rapid growth on lactate. This strain and the five adaptively evolved strains had four to eight-fold higher transcript abundance than wild-type cells for genes for the two subunits of succinyl-CoA synthase, an enzyme required for growth on lactate. DNA-binding assays demonstrated that the protein encoded by GSU0514 bound to the putative promoter of the succinyl-CoA synthase operon. The binding sequence was not apparent elsewhere in the genome. These results demonstrate that a single-base-pair mutation in a transcriptional regulator can have a significant impact on the capacity for substrate utilization and suggest that adaptive evolution should be considered as a potential response of microorganisms to environmental change(s) imposed during bioremediation.
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Other studies reported improved growth on many non-evolutionary carbon sources after batch selection with gluconeogenic carbon sources.
“whereas other studies reported improved growth on many non-evolutionary carbon sources after batch selection with gluconeogenic carbon sources [66,67].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [66]
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Adaptive laboratory evolution—Principles and applications for biotechnology
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- Authors
- Summers, Z. M., Ueki, T., Ismail, W., Haveman, S. A., & Lovley, D. R.
- Affiliations
- University of Massachusetts Amherst (US)
- Arabian Gulf University (BH)
- Luca Technologies Inc. , Golden, CO , USA
- Corresponding author
- Summers, Zarath M
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-11-24
- Volume
- 6
- Issue
- 5
- Pages
- 975-983
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- PMC
- PMC3329115
- OpenAlex
- W2085912383
- Cited by
- 39
- References
- 58
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 3 · 2020: 6 · 2019: 7 · 2018: 1 · 2017: 3 · 2015: 2 · 2013: 3 · 2012: 4
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The ISME Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy (FC02-02ER63446)
- Office of Science
- Topics
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- Geobacter sulfurreducens, lactate metabolism, experimental evolution, adaptive evolution, transcription factors, single nucleotide polymorphisms, anaerobic bioremediation, groundwater bioremediation, DNA binding, promoter regulation, substrate utilization
- MeSH terms
- Adaptation, Biological, Amino Acid Sequence, Base Sequence, Biodegradation, Environmental, DNA, Bacterial, Molecular Sequence Data, Mutation, Operon, Promoter Regions, Genetic, Sequence Analysis, DNA, Evolution, Molecular, Lactic Acid, Base Pairing, Geobacter, Groundwater
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