Cited by 134Traumatic Brain Injury and Neurovascular DisturbancesTraumatic Brain Injury ResearchBarrier Structure and Function Studies

An Analysis of Regional Microvascular Loss and Recovery following Two Grades of Fluid Percussion Trauma: A Role for Hypoxia-Inducible Factors in Traumatic Brain Injury

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Park, Eugene, Bell, Joshua D, Siddiq, Ishita P, Baker, Andrew J·2009·Journal of Cerebral Blood Flow & Metabolism

Abstract

Secondary hypoxic/ischemic injuries, stemming from reductions in cerebral blood flow are important contributing factors in progressive neuronal dysfunction after brain trauma. A greater preclinical understanding of how brain trauma leads to secondary hypoxia/ischemia is necessary in the development of posttraumatic brain injury (TBI) therapeutics. To this end, we examined the density of microvascular coverage in the injured and contralateral cortical hemispheres using two intensities of fluid percussion trauma in rats. A silicone microangiography technique showed a significant loss in microvascular density in 2 atmosphere (atm) (16.9±3.8%) and 3 atm (15.7±1.3%) injured animals relative to sham animals (29.9±2.5%; P<0.01). RECA-1 immunohistochemistry indicated that capillary changes involved a reduction in capillary number and diameter. Reduction in microvascular density was shown to be a diffuse phenomenon occurring up to 4 mm rostral and caudal to the injury epicenter. Recovery of microvasculature occurred by 2 weeks after injury only in the 2 atm injury group. Expression of HIF1α and increased vascular endothelial growth factor expression were observed in the ipsilateral hippocampus suggesting sufficiently impaired microcirculation resulting in the expression of hypoxic-response proteins. Collectively, the results indicate diffuse and heterogeneous microvascular alterations as well as endogenous expression of neuroprotective and neovascularization pathways after TBI.

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Crossref · 10.1038/jcbfm.2008.151

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  • In animal models of TBI, reduced microvascular densities were observed in the brain within a day of injury, although the density returned to normal within 2 weeks, suggesting engagement of vascular repair mechanisms.

    “In animal models of TBI, reduced microvascular densities were observed in the brain within a day of injury, although the density returned to normal within 2 weeks, suggesting engagement of vascular repair mechanisms (129).”

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Authors
Park, E., Bell, J. D., Siddiq, I. P., & Baker, A. J.
Affiliations
St. Michael's Hospital (CA)
Corresponding author
Park, Eugene
Venue
Journal of Cerebral Blood Flow & Metabolism
Published
2008-12-17
Volume
29
Issue
3
Pages
575-584
ISSN
  • 0271-678X (ISSN-L)
  • 1559-7016
OpenAlex
W2017778182
Cited by
134
References
41
Citations by year
2026: 5 · 2025: 8 · 2024: 12 · 2023: 9 · 2022: 6 · 2021: 10 · 2020: 10 · 2019: 8 · 2018: 7 · 2017: 11 · 2016: 6 · 2015: 6 · 2014: 10 · 2013: 7 · 2012: 9
FWCI
2.12
Type
journal article
Language
en
Open access
Locations
  • Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
  • Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
traumatic brain injury, fluid percussion injury, microvessel density, cerebral microcirculation, hypoxia-inducible factor-1α, vascular endothelial growth factor, capillary density, neovascularization, hippocampus, microangiography
MeSH terms
Animals, Brain Injuries, Cerebral Angiography, Cerebrovascular Circulation, Disease Models, Animal, Immunohistochemistry, Male, Microcirculation, Percussion, Pressure, Blotting, Western, Rats, Sprague-Dawley, Vascular Endothelial Growth Factor A, Rats, Hypoxia-Inducible Factor 1, alpha Subunit, Microvessels

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