The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Engineering microbes to sense and eradicate Pseudomonas aeruginosa , a human pathogen
Open accessAbstract
Synthetic biology aims to systematically design and construct novel biological systems that address energy, environment, and health issues. Herein, we describe the development of a synthetic genetic system, which comprises quorum sensing, killing, and lysing devices, that enables Escherichia coli to sense and kill a pathogenic Pseudomonas aeruginosa strain through the production and release of pyocin. The sensing, killing, and lysing devices were characterized to elucidate their detection, antimicrobial and pyocin release functionalities, which subsequently aided in the construction of the final system and the verification of its designed behavior. We demonstrated that our engineered E. coli sensed and killed planktonic P. aeruginosa, evidenced by 99% reduction in the viable cells. Moreover, we showed that our engineered E. coli inhibited the formation of P. aeruginosa biofilm by close to 90%, leading to much sparser and thinner biofilm matrices. These results suggest that E. coli carrying our synthetic genetic system may provide a novel synthetic biology-driven antimicrobial strategy that could potentially be applied to fighting P. aeruginosa and other infectious pathogens.
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Citations
Cites
1 retracted
Reprogramming bacteria to seek and destroy an herbicide
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Cited by
Hwang, I. Y., Koh, E., Wong, A., March, J. C., Bentley, W. E., Lee, Y. S., & Chang, M. W. (2017) · Nature Communications · doi:10.1038/ncomms15028 · Cited by 472
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Engineered commensal microbes for diet-mediated colorectal-cancer chemoprevention
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Self-citationMining the microbiota for microbial and metabolite-based immunotherapies
Skelly, A. N., Sato, Y., Kearney, S., & Honda, K. (2019) · Nature Reviews Immunology · doi:10.1038/s41577-019-0144-5 · Cited by 306
Palmer, J. D., Piattelli, E., McCormick, B. A., Silby, M. W., Brigham, C. J., & Bucci, V. (2018) · ACS Infectious Diseases · doi:10.1021/acsinfecdis.7b00114 · Cited by 133
Bottom-Up Approaches to Synthetic Cooperation in Microbial Communities
Rodríguez Amor, D., & Dal Bello, M. (2019) · Life · doi:10.3390/life9010022 · Cited by 71
Al-Shaarani, A. A. Q. A., & Pecoraro, L. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1428415 · Cited by 50
Comparative Genomics and Pan-Genome Driven Prediction of a Reduced Genome of Akkermansia muciniphila
Bukhari, S. A. R., Irfan, M., Ahmad, I., & Chen, L. (2022) · Microorganisms · doi:10.3390/microorganisms10071350 · Cited by 18
Metadata
- Authors
- Saeidi, N., Wong, C. K., Lo, T., Nguyen, H. X., Ling, H., Leong, S. S. J., Poh, C. L., & Chang, M. W.
- Venue
- Molecular Systems Biology
- Published
- 2011-01
- Volume
- 7
- Issue
- 1
- Pages
- 521-521
- ISSN
- 1744-4292 (ISSN-L)
- OpenAlex
- W2143337671
- Cited by
- 369
- References
- 26
- FWCI
- 9.87
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
