The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Myoblasts Derived From Normal hESCs and Dystrophic hiPSCs Efficiently Fuse With Existing Muscle Fibers Following Transplantation
Open accessAbstract
Human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) have an endless self-renewal capacity and can theoretically differentiate into all types of lineages. They thus represent an unlimited source of cells for therapies of regenerative diseases, such as Duchenne muscular dystrophy (DMD), and for tissue repair in specific medical fields. However, at the moment, the low number of efficient specific lineage differentiation protocols compromises their use in regenerative medicine. We developed a two-step procedure to differentiate hESCs and dystrophic hiPSCs in myogenic cells. The first step was a culture in a myogenic medium and the second step an infection with an adenovirus expressing the myogenic master gene MyoD. Following infection, the cells expressed several myogenic markers and formed abundant multinucleated myotubes in vitro. When transplanted in the muscle of Rag/mdx mice, these cells participated in muscle regeneration by fusing very well with existing muscle fibers. Our findings provide an effective method that will permit to use hESCs or hiPSCs for preclinical studies in muscle repair.
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Cites
Dystrophin: the protein product of the Duchenne muscular dystrophy locus
Hoffman, E. P., Brown, R. H., & Kunkel, L. M. (1987) · Cell · doi:10.1016/0092-8674(87)90579-4 · Cited by 4,693
Disease-Specific Induced Pluripotent Stem Cells.
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Somatic coding mutations in human induced pluripotent stem cells
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Feeder-independent culture of human embryonic stem cells
Ludwig, T. E., Bergendahl, V., Levenstein, M. E., Yu, J., Probasco, M. D., & Thomson, J. A. (2006) · Nature Methods · doi:10.1038/nmeth902 · Cited by 681
Defining the nature of human pluripotent stem cell progeny
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Cited by
Li, H. L., Fujimoto, N., Sasakawa, N., Shirai, S., Ohkame, T., Sakuma, T., Tanaka, M., Amano, N., Watanabe, A., Sakurai, H., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.10.013 · Cited by 471
Engineering human pluripotent stem cells into a functional skeletal muscle tissue
Rao, L., Qian, Y., Khodabukus, A., Ribar, T., & Bursac, N. (2018) · Nature Communications · doi:10.1038/s41467-017-02636-4 · Cited by 334
Chal, J., Al Tanoury, Z., Hestin, M., Gobert, B., Aivio, S., Hick, A., Cherrier, T., Nesmith, A. P., Parker, K. K., & Pourquié, O. (2016) · Nature Protocols · doi:10.1038/nprot.2016.110 · Cited by 299
Borchin, B., Chen, J., & Barberi, T. (2013) · Stem Cell Reports · doi:10.1016/j.stemcr.2013.10.007 · Cited by 184
Zhao, C., Farruggio, A. P., Bjornson, C. R. R., Chavez, C. L., Geisinger, J. M., Neal, T. L., Karow, M., & Calos, M. P. (2014) · PLoS ONE · doi:10.1371/journal.pone.0096279 · Cited by 25
Metadata
- Authors
- Goudenege, S., Lebel, C., Huot, N. B., Dufour, C., Fujii, I., Gekas, J., Rousseau, J., & Tremblay, J. P.
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 20
- Issue
- 11
- Pages
- 2153-2167
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2053328538
- Cited by
- 173
- References
- 5
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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