The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Engineering human pluripotent stem cells into a functional skeletal muscle tissue
Open accessAbstract
The generation of functional skeletal muscle tissues from human pluripotent stem cells (hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) via transient overexpression of Pax7 in paraxial mesoderm cells differentiated from hPSCs. In 2D culture, iMPCs readily differentiate into spontaneously contracting multinucleated myotubes and a pool of satellite-like cells endogenously expressing Pax7. Under optimized 3D culture conditions, iMPCs derived from multiple hPSC lines reproducibly form functional skeletal muscle tissues (iSKM bundles) containing aligned multi-nucleated myotubes that exhibit positive force–frequency relationship and robust calcium transients in response to electrical or acetylcholine stimulation. During 1-month culture, the iSKM bundles undergo increased structural and molecular maturation, hypertrophy, and force generation. When implanted into dorsal window chamber or hindlimb muscle in immunocompromised mice, the iSKM bundles survive, progressively vascularize, and maintain functionality. iSKM bundles hold promise as a microphysiological platform for human muscle disease modeling and drug development.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,926
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Ultrasensitive fluorescent proteins for imaging neuronal activity
Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Gilbert, P. M., Havenstrite, K. L., Magnusson, K. E. G., Sacco, A., Leonardi, N. A., Kraft, P., Nguyen, N. K., Thrun, S., Lutolf, M. P., & Blau, H. M. (2010) · Science · doi:10.1126/science.1191035 · Cited by 1,587
An Expanded Palette of Genetically Encoded Ca 2+ Indicators
Zhao, Y., Araki, S., Wu, J., Teramoto, T., Chang, Y.-F., Nakano, M., Abdelfattah, A. S., Fujiwara, M., Ishihara, T., Nagai, T., et al. (2011) · Science · doi:10.1126/science.1208592 · Cited by 1,260
Building Muscle: Molecular Regulation of Myogenesis
Bentzinger, C. F., Wang, Y. X., & Rudnicki, M. A. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a008342 · Cited by 1,195
Calcium Ion in Skeletal Muscle: Its Crucial Role for Muscle Function, Plasticity, and Disease
Berchtold, M. W., Brinkmeier, H., & Müntener, M. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.3.1215 · Cited by 999
Three muscular dystrophies: loss of cytoskeleton-extracellular matrix linkage
Campbell, K. P. (1995) · Cell · doi:10.1016/0092-8674(95)90344-5 · Cited by 829
Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
Relaix, F., Montarras, D., Zaffran, S., Gayraud-Morel, B., Rocancourt, D., Tajbakhsh, S., Mansouri, A., Cumano, A., & Buckingham, M. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200508044 · Cited by 736
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“, 2019) and derived from human iPSCs (Rao et al., 2018).”
BackgroundDeveloping defined substrates for stem cell culture and differentiation
Hagbard, L., Cameron, K., August, P., Penton, C., Parmar, M., Hay, D. C., & Kallur, T. (2018) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2017.0230 · Cited by 62
Kirby, T. J. (2019) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01031.2018 · Cited by 37
iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling
Guo, D., Daman, K., Chen, J. J., Shi, M.-J., Yan, J., Matijasevic, Z., Rickard, A. M., Bennett, M. H., Kiselyov, A., Zhou, H., et al. (2022) · eLife · doi:10.7554/elife.70341 · Cited by 26
Organotypic cultures as aging associated disease models
Sanchez, M. M., Bagdasarian, I. A., Darch, W., & Morgan, J. T. (2022) · Aging · doi:10.18632/aging.204361 · Cited by 12
Metadata
- Authors
- Rao, L., Qian, Y., Khodabukus, A., Ribar, T., & Bursac, N.
- Venue
- Nature Communications
- Published
- 2018-01-09
- Volume
- 9
- Issue
- 1
- Pages
- 126-126
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2781902139
- Cited by
- 334
- References
- 60
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- gold
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