Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

Genome engineering using the CRISPR-Cas9 system

Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

The new frontier of genome engineering with CRISPR-Cas9

Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062

Development and Applications of CRISPR-Cas9 for Genome Engineering

Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762

Improved vectors and genome-wide libraries for CRISPR screening

Sanjana, N. E., Shalem, O., & Zhang, F. (2014) · Nature Methods · doi:10.1038/nmeth.3047 · Cited by 5,239

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741

A CRISPR/Cas9-based enhancement of high-throughput single-cell transcriptomics

Pandey, A. C., Bezney, J., DeAscanis, D., Kirsch, E., Ahmed, F., Crinklaw, A., Choudhary, K. S., Mandala, T., Deason, J., Hamidi, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-59880-2 · Cited by 16

An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo

Qin, J., Xu, H., Zhang, P., Zhang, C., Zhu, Z., Qu, R., Qin, Y., & Zeng, W. (2015) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-015-0058-4 · Cited by 13

Gene editing of stem cells for kidney disease modelling and therapeutic intervention

Lau, R. W., Wang, B., & Ricardo, S. D. (2018) · Nephrology · doi:10.1111/nep.13410 · Cited by 14

Infections after xenotransplantation

Nellore, A. (2018) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000580 · Cited by 13

Functional analysis of the UL24 protein of suid herpesvirus 1

Ye, C., Chen, J., Cheng, X., Zhou, S., Jiang, S., Xu, J., Zheng, H., Tong, W., Li, G., & Tong, G. (2018) · Virus Genes · doi:10.1007/s11262-018-1619-3 · Cited by 13

CRISPR/Cas9 guided genome and epigenome engineering and its therapeutic applications in immune mediated diseases

Singh, D. D., Hawkins, R. D., Lahesmaa, R., & Tripathi, S. K. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.05.007 · Cited by 13

Heparan sulfate controls skeletal muscle differentiation and motor functions

Yokoyama, M., Matsuzawa, T., Yoshikawa, T., Nunomiya, A., Yamaguchi, Y., & Yanai, K. (2020) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2020.129707 · Cited by 13

Modeling Non-Alcoholic Fatty Liver Disease (NAFLD) Using “Good-Fit” Genome-Editing Tools

Kim, U., Kim, N., & Shin, H. Y. (2020) · Cells · doi:10.3390/cells9122572 · Cited by 13

RNA‐Cleaving DNA Thresholder Controlled by Concentrations of miRNA Cancer Marker

de Oliveira, A. G. G., Dubovichenko, M. V., ElDeeb, A. A., Wanjohi, J., Zablotskaya, S. S., & Kolpashchikov, D. M. (2021) · ChemBioChem · doi:10.1002/cbic.202000769 · Cited by 13

New Perspectives of Gene Therapy on Polyglutamine Spinocerebellar Ataxias: From Molecular Targets to Novel Nanovectors

Borbolla‐Jiménez, F. V., Prado-Audelo, M. L. D., Cisneros, B., Caballero-Florán, I. H., Leyva‐Gómez, G., & Magaña, J. J. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13071018 · Cited by 13

Reflections on drug resistance to KRAS inhibitors and gene silencing/editing tools for targeting mutant KRAS in cancer treatment

Han, Z., Zhou, D., Wang, J., Jiang, B., & Liu, X. (2022) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2022.188677 · Cited by 13

Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions

Jamehdor, S., Pajouhanfar‎, S., Saba, S., Uzan, G., Teimoori, A., & Naserian, S. (2022) · Cells · doi:10.3390/cells11060999 · Cited by 13

Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast

Guang, C., Lin, Z., & Shi, S. (2022) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2022.110056 · Cited by 14

Complete and Prolonged Inhibition of Herpes Simplex Virus Type 1 Infection In Vitro by CRISPR/Cas9 and CRISPR/CasX Systems

Karpov, D. S., Demidova, N. A., Kulagin, K. A., Shuvalova, A. I., Kovalev, M. A., Simonov, R. A., Карпов, В. Л., Snezhkina, A. V., Kudryavtseva, A. V., Рафаиловна, К. Р., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232314847 · Cited by 13

Progress in and Prospects of Genome Editing Tools for Human Disease Model Development and Therapeutic Applications

Phan, H. T. L., Kim, K., Lee, H., & Seong, J. K. (2023) · Genes · doi:10.3390/genes14020483 · Cited by 13

Stem Cell Engineering and Differentiation for Disease Modeling and Cell-based Therapies

Randolph, L. N., Jiang, Y., Lian, X. L., & equally., §. T. A. C. (2017) · AIMS Cell and Tissue Engineering · doi:10.3934/celltissue.2017.2.140 · Cited by 13

Cell and Gene Therapies for Mucopolysaccharidoses: Base Editing and Therapeutic Delivery to the CNS

Christensen, C. L., Ashmead, R. E., & Choy, F. Y. M. (2019) · Diseases · doi:10.3390/diseases7030047 · Cited by 13

Pleiotropy Complicates Human Gene Editing: CCR5Δ32 and Beyond

Li, T., & Shen, X. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2019.00669 · Cited by 13

The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy

Sanches-da-Silva, G. D. N., Medeiros, L. F. S., & Lima, F. M. (2019) · International Journal of Genomics · doi:10.1155/2019/8458263 · Cited by 13

Designer Nucleases: Gene-Editing Therapies using CCR5 as an Emerging Target in HIV

Almeida, M. J., & Matos, A. M. (2019) · Current HIV Research · doi:10.2174/1570162x17666191025112918 · Cited by 13

Utilization of CRISPR/Cas9 gene editing in cellular therapies for lymphoid malignancies

Mehravar, M., Roshandel, E., Salimi, M., Chegeni, R., Gholizadeh, M., Mohammadi, M. H., & Hajifathali, A. (2020) · Immunology Letters · doi:10.1016/j.imlet.2020.07.003 · Cited by 13

Everything in moderation, even hype: learning from vaccine controversies to strike a balance with CRISPR

Benston, S. (2017) · Journal of Medical Ethics · doi:10.1136/medethics-2016-103666 · Cited by 14

Correction of the aprt Gene Using Repair-Polypurine Reverse Hoogsteen Hairpins in Mammalian Cells

Félix, A. J., Ciudad, C. J., & Noé, V. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.12.015 · Cited by 13

DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolutionPreprint

Elacqua, J. J., Ranu, N., DiIorio, S. E., & Blainey, P. C. (2020) · Genome Research · doi:10.1101/gr.265223.120 · Cited by 14

Precision therapeutics for inflammatory bowel disease using engineered probiotics: Strategies and optimization

Xue, Y., Hu, M., Cha, S., Xue, C., & Dong, N. (2025) · Acta Biomaterialia · doi:10.1016/j.actbio.2025.10.012 · Cited by 13

Current genetic strategies to investigate gene functions in Trichoderma reesei

Ma, C., Liu, J., Tang, J., Sun, Y., Jiang, X., Zhang, T., Yan, F., Liu, Q., & Wang, L. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02104-3 · Cited by 12

Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks

Bi, C., Yuan, B., Zhang, Y., Wang, M., Tian, Y., & Li, M. (2025) · Communications Biology · doi:10.1038/s42003-025-07539-5 · Cited by 12

Prenatal gene editing for neurodevelopmental diseases: Ethical considerations

Major, R., & Juengst, E. T. (2025) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2025.01.003 · Cited by 14

Genetic editing of the virulence gene of Escherichia coli using the CRISPR system

Hou, M., Sun, S., Feng, Q., Dong, X., Zhang, P., Shi, B., Liu, J., & Shi, D. (2020) · PeerJ · doi:10.7717/peerj.8881 · Cited by 13

Genome Editing in Medicine: Tools and Challenges

Petraitytė, G., Preikšaitienė, E., & Mikštienė, V. (2021) · Acta medica Lituanica · doi:10.15388/amed.2021.28.2.8 · Cited by 12

Functional interrogation of autoimmune disease genetics using CRISPR/Cas9 technologies and massively parallel reporter assays

Ding, J., Frantzeskos, A., & Orozco, G. (2021) · Seminars in Immunopathology · doi:10.1007/s00281-021-00887-4 · Cited by 12

CRISPR/Cas9-mediated Inactivation of arginase in a yeast strain isolated from Nuruk and its impact on the whole genome

Chin, Y., Shin, S. C., Han, S., Jang, H. W., & Kim, H. J. (2021) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2021.09.019 · Cited by 12

Genome‐wide off‐target analyses of CRISPR/Cas9‐mediated T‐cell receptor engineering in primary human T cells

Kaeuferle, T., Stief, T. A., Canzar, S., Kutlu, N. N., Willier, S. M., Stenger, D., Ferrada‐Ernst, P., Habjan, N., Peters, A. E., Busch, D. H., et al. (2022) · Clinical & Translational Immunology · doi:10.1002/cti2.1372 · Cited by 12

CRISPR Technology in Cancer Diagnosis and Treatment: Opportunities and Challenges

Shademan, B., Masjedi, S., Karamad, V., Isazadeh, A., Söğütlü, F., rad, M. H. S., & Nourazarian, A. (2022) · Biochemical Genetics · doi:10.1007/s10528-022-10193-9 · Cited by 12

Characterizing genes associated with cancer using the CRISPR/Cas9 system: A systematic review of genes and methodological approaches

Gonzalez-Salinas, F., Martínez-Amador, C., & Treviño, V. (2022) · Gene · doi:10.1016/j.gene.2022.146595 · Cited by 12

Comparative structural and dynamics study of free and gRNA-bound FnCas9 and SpCas9 proteins

Panda, G., & Ray, A. (2022) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2022.07.041 · Cited by 12

Engineering T Cell Development for the Next Generation of Stem Cell-Derived Immunotherapies

Michaels, Y. S., Durland, L. J., & Zandstra, P. W. (2023) · GEN Biotechnology · doi:10.1089/genbio.2023.0008 · Cited by 12

Genetic manipulation of PLB-985 cells and quantification of chemotaxis using the underagarose assay

Saunders, C. A., Majumdar, R., Molina-Aponte, Y., Subramanian, B. C., & Parent, C. A. (2018) · Methods in cell biology · doi:10.1016/bs.mcb.2018.09.002 · Cited by 12

Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1

Inwood, S., Abaandou, L., Betenbaugh, M. J., & Shiloach, J. (2019) · New Biotechnology · doi:10.1016/j.nbt.2019.08.004 · Cited by 12

Recent advances in stem cells and gene editing: Drug discovery and therapeutics

Bayarsaikhan, D., Bayarsaikhan, G., & Lee, B. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.019 · Cited by 12

The genomes of precision edited cloned calves show no evidence for off-target events or increased de novo mutagenesis

Jivanji, S., Harland, C., Cole, S., Brophy, B., Garrick, D. J., Snell, R. G., Littlejohn, M. D., & Laible, G. (2021) · BMC Genomics · doi:10.1186/s12864-021-07804-x · Cited by 12

CRISPR/Cas9 small promoter deletion in H19 lncRNA is associated with altered cell morphology and proliferation

Santos, R. D. S., Pinheiro, D. P., Teixeira, L. P. R., Sales, S. L. A., Luciano, M. C. D. S., Melo, M. M. D. L., Pinheiro, R. F., Tavares, K. C. S., Furtado, G. P., Pessoa, C., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-97058-0 · Cited by 12

Hierarchically tumor-activated nanoCRISPR-Cas13a facilitates efficient microRNA disruption for multi-pathway-mediated tumor suppression

Liu, X., Yang, S., Wang, L., Wu, X., Wang, X., Ou, C., Yang, J. P., Song, L., Zhou, S., Wu, Q., et al. (2023) · Theranostics · doi:10.7150/thno.81776 · Cited by 11

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Smirnov, K., Weiß, F., Hatzl, A.-M., Rieder, L., Olesen, K., Jensen, S., & Glieder, A. (2024) · Journal of Fungi · doi:10.3390/jof10030197 · Cited by 11

Plant Breeding Using the CRISPR-Cas9 System for Food Security and Facing Climate Change

Ambika, A., Bhati, S., & Kumar, R. (2024) · Interdisciplinary biotechnological advances · doi:10.1007/978-981-99-9338-3_6 · Cited by 11

Deletion of CD44 promotes adipogenesis by regulating PPARγ and cell cycle-related pathways

Weng, X., Jiang, H., Walker, D. J., Zhou, H., Lin, D., Wang, J., & Kang, L. (2024) · Journal of Endocrinology · doi:10.1530/joe-24-0079 · Cited by 11

Emerging Strategies to Overcome Chemoresistance: Structural Insights and Therapeutic Targeting of Multidrug Resistance-Linked ATP-Binding Cassette Transporters

Nagampalli, R. S. K., Vadla, G. P., & Nadendla, E. K. (2025) · International Journal of Translational Medicine · doi:10.3390/ijtm5010006 · Cited by 13

CRISPR-MFH: A Lightweight Hybrid Deep Learning Framework with Multi-Feature Encoding for Improved CRISPR-Cas9 Off-Target Prediction

Zheng, Y., Zou, Q., Li, J., & Yang, Y. (2025) · Genes · doi:10.3390/genes16040387 · Cited by 11

Modulating Collagen I Expression in Fibroblasts by CRISPR-Cas9 Base Editing of the Collagen 1A1 Promoter

Daliri, K., Hescheler, J., Newby, G. A., Clement, K., Liu, D. R., & Pfannkuche, K. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26073041 · Cited by 11

Gene Editing in Ganoderma lucidum: Development, Challenges, and Future Prospects

He, S., Liu, Y., Zhang, Z., Cai, M., Hao, Y., & Hu, H. (2025) · Journal of Fungi · doi:10.3390/jof11040310 · Cited by 11

Shining light on CRISPR/Cas9 therapeutics for inherited retinal diseases

Geiger, A., Kennedy, J. G., Staker, L. G., Wensel, T. G., Casson, R. J., & Thomas, P. Q. (2025) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2025.101376 · Cited by 11

In vitro functional genetic modification of canine adenovirus type 2 genome by CRISPR/Cas9

Sajib, A. M., Agarwal, P., Patton, D. J., Nance, R. L., Stahr, N. A., Kretzschmar, W. P., Sandey, M., & Smith, B. F. (2021) · Laboratory Investigation · doi:10.1038/s41374-021-00654-x · Cited by 11

Genome Editing among Bioethics and Regulatory Practices

Mandrioli, M. (2021) · Biomolecules · doi:10.3390/biom12010013 · Cited by 15

Genetic Kidney Diseases (GKDs) Modeling Using Genome Editing Technologies

Gómez-García, F., Pulleiro, R. M., Carrera, N., Allegue, C., & García-González, M. Á. (2022) · Cells · doi:10.3390/cells11091571 · Cited by 13

Transplantation of Adipose-Tissue-Engineered Constructs with CRISPR-Mediated UCP1 Activation

Michurina, S. S., Stafeev, I., Boldyreva, M. A., Truong, V. A., Ratner, E. I., Menshikov, M. Y., Hu, Y., & Parfyonova, Y. V. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24043844 · Cited by 11

Research and Therapeutic Approaches in Stem Cell Genome Editing by CRISPR Toolkit

Mollashahi, B., Latifi‐Navid, H., Owliaee, I., Shamdani, S., Uzan, G., Jamehdor, S., & Naserian, S. (2023) · Molecules · doi:10.3390/molecules28041982 · Cited by 11

Gene Editing as the Future of Cardiac Amyloidosis Therapeutics

Sethi, Y., Mahtani, A. U., Khehra, N., Padda, I., Patel, N., Sebastian, S. A., Malhi, G., Kaiwan, O., Saith, S. E., & Johal, G. (2023) · Current Problems in Cardiology · doi:10.1016/j.cpcardiol.2023.101741 · Cited by 11

CRISPR-Cas attack of HIV-1 proviral DNA can cause unintended deletion of surrounding cellular DNA

Liu, Y., Binda, C. S., Berkhout, B., & Das, A. T. (2023) · Journal of Virology · doi:10.1128/jvi.01334-23 · Cited by 13

gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 Systems Refinement

Motoche-Monar, C., Ordoñez, J. E., Chang, O., & Gonzales-Zubiate, F. A. (2023) · Biomolecules · doi:10.3390/biom13121698 · Cited by 12

The role of epigenetic mechanisms in the long‐term effects of early‐life adversity and mother–infant relationship on physiology and behavior of offspring in laboratory rats and mice

Burenkova, O. V., & Grigorenko, E. L. (2024) · Developmental Psychobiology · doi:10.1002/dev.22479 · Cited by 11

Cas9 nickase–mediated contraction of CAG/CTG repeats is associated with phenotypic improvements in a Huntington’s disease mouse model

Murillo, A., Alpaugh, M., Durairaj, R. R. P., Randall, E. L., Larin, M., Heraty, L., Aston, A. N., Taylor, A. S., Monteys, A. M., Stöberl, N., et al. (2026) · Science Translational Medicine · doi:10.1126/scitranslmed.aef9466 · Cited by 11

RNA-Seq Analysis of an Antisense Sequence Optimized for Exon Skipping in Duchenne Patients Reveals No Off-Target Effect

Domenger, C., Allais, M., François, V., Léger, A., Lecomte, É., Montus, M., Servais, L., Voït, T., Moullier, P., Audic, Y., et al. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.12.008 · Cited by 11

Loss of tumor intrinsic PD-L1 confers resistance to drug-induced apoptosis in human colon cancer

Feng, D., Chen, Z., He, X., Huang, S., & Zhang, Z. (2020) · Neoplasma · doi:10.4149/neo_2020_200531n589 · Cited by 11

Integrating Biomaterials and Genome Editing Approaches to Advance Biomedical Science

Abdeen, A. A., Cosgrove, B. D., Gersbach, C. A., & Saha, K. (2021) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-122019-121602 · Cited by 11

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER Twenty-five years after Dolly: how far have we come?

Klinger, B., & Schnieke, A. (2021) · Reproduction · doi:10.1530/rep-20-0652 · Cited by 11

Critical cancer vulnerabilities identified by unbiased CRISPR/Cas9 screens inform on efficient cancer Immunotherapy

Potts, M. A., McDonald, J. A., Sutherland, K. D., & Herold, M. J. (2020) · European Journal of Immunology · doi:10.1002/eji.202048712 · Cited by 11

Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines

Bownes, L. V., Williams, A. P., Marayati, R., Quinn, C. H., Hutchins, S. C., Stewart, J. E., Vu, T., Easlick, J. L., Mroczek‐Musulman, E., Crossman, D. K., et al. (2021) · Cancers · doi:10.3390/cancers13133201 · Cited by 11

DNA nanotechnology-based strategies for minimising hybridisation-dependent off-target effects in oligonucleotide therapies

Li, X., Hu, H., Wang, H., Liu, J., Jiang, W., Zhou, F., & Zhang, J. (2024) · Materials Horizons · doi:10.1039/d4mh01158a · Cited by 12

Streamlined protoplast transfection system for in-vivo validation and transgene-free genome editing in Banana

Lakhani, H., Kumar, N., Jangra, A., Negi, S., Dholariya, T., & Tiwari, S. (2025) · Transgenic Research · doi:10.1007/s11248-025-00446-9 · Cited by 10

Controlling DNA–RNA strand displacement kinetics with base distribution

Ratajczyk, E. J., Bath, J., Šulc, P., Doye, J. P. K., Louis, A. A., & Turberfield, A. J. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2416988122 · Cited by 10

Multidrug Resistance: Are We Still Afraid of the Big Bad Wolf

Alhazza, A., Oyegbesan, A., Bousoik, E., & Aliabadi, H. M. (2025) · Pharmaceuticals · doi:10.3390/ph18060895 · Cited by 12

CRISPR/Cas9 a genomic engineering technology for treatment in ALS mouse models

Khan, H., Riaz, H., Ahmed, A., Kiyani, M. M., Jawad, S. M., Shah, S. S. U. D., Abualait, T., Al‐Hussain, F., Li, H., & Bashir, S. (2025) · Regenerative Therapy · doi:10.1016/j.reth.2025.07.009 · Cited by 10

Hypoimmune stem cells and islets: hype or a true breakthrough in diabetes treatment?

Shalaby, K. E., & Abdelalim, E. M. (2025) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-025-00786-8 · Cited by 10

Establishment of a conditional TALEN system using the translational enhancer dMac3 and an inducible promoter activated by glucocorticoid treatment to increase the frequency of targeted mutagenesis in plants

Onodera, H., Shingu, S., Ohnuma, M., Horie, T., Kihira, M., Kusano, H., Teramura, H., & Shimada, H. (2018) · PLoS ONE · doi:10.1371/journal.pone.0208959 · Cited by 10

CRISPR/Cas: a potential gene-editing tool in the nervous system

Gao, Y., Gao, K., & Yang, H. (2020) · Cell Regeneration · doi:10.1186/s13619-020-00044-6 · Cited by 11

“Cerberus” T Cells: A Glucocorticoid-Resistant, Multi-Pathogen Specific T Cell Product to Fight Infections in Severely Immunocompromised Patients

Koukoulias, K., Papayanni, P. G., Georgakopoulou, A., Alvanou, M., Laidou, S., Kouimtzidis, A., Pantazi, C., Gkoliou, G., Vyzantiadis, T., Spyridonidis, A., et al. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2020.608701 · Cited by 10

A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells

Schmieder, V., Novak, N., Dhiman, H., Ly, N. N., Serafimova, E., Klanert, G., Baumann, M., Kildegaard, H. F., & Borth, N. (2021) · Biotechnology Reports · doi:10.1016/j.btre.2021.e00649 · Cited by 10

Genomic knockout of hsp23 both decreases and increases fitness under opposing thermal extremes in Drosophila melanogaster

Gu, X., Chen, W., Perry, T., Batterham, P., & Hoffmann, A. A. (2021) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2021.103652 · Cited by 10

Unexpected gene activation following CRISPR‐Cas9‐mediated genome editing

Manjón, A. G., Linder, S., Teunissen, H., Friskes, A., Zwart, W. T., de Wit, E., & Medema, R. H. (2021) · EMBO Reports · doi:10.15252/embr.202153902 · Cited by 10

Genetically engineered cellular models of prion propagation

Arshad, H., & Watts, J. C. (2022) · Cell and Tissue Research · doi:10.1007/s00441-022-03630-z · Cited by 10

Dynamic observations of CRISPR‐Cas target recognition and cleavage heterogeneities

Zhang, Z., Jeong, H., Zu, D., Zhao, X., Senaratne, P., Filbin, J., Silber, B., Kang, S., Gladstone, A., Lau, M. S., et al. (2022) · Nanophotonics · doi:10.1515/nanoph-2022-0286 · Cited by 10

Strategies for generation of mice via CRISPR/HDR-mediated knock-in

Mathew, S. M. (2023) · Molecular Biology Reports · doi:10.1007/s11033-023-08278-8 · Cited by 10

CRISPR-Cas genome editing for the development of abiotic stress-tolerant wheat

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Nanoparticle-mediated delivery of non-viral gene editing technology to the brain

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Bioinformatics for Plant Genetics and Breeding Research

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CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers

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Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation

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CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation

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Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection

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Gene editing and modulation for Duchenne muscular dystrophy

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Novel Approaches for Genome Editing to Develop Climate Smart Crops

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ADAR-Mediated RNA Editing and Its Therapeutic Potentials

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Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 in Komagataella phaffii: A Budding Yeast in Distress

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Mutant Cas9-transcriptional activator activates HIV-1 in U1 cells in the presence and absence of LTR-specific guide RNAs

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The Strategies and Challenges of CCR5 Gene Editing in Hematopoietic Stem and Progenitor Cells for the Treatment of HIV

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Role and application of CRISPR-Cas9 in the management of Alzheimer’s disease

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A Review of Gene Therapies for Hemoglobinopathies

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CRISPR challenges in clinical developments

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CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects

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A targeted approach: Gene and RNA editing for neurodegenerative disease treatment

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CRISPR/ Cas9 Off-targets: Computational Analysis of Causes, Prediction, Detection, and Overcoming Strategies

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Progress of application and off-target effects of CRISPR/Cas9.

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Emerging strategies to minimize the off-target effects in CRISPR/Cas9 system

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Off-target effects in CRISPR/Cas9 gene editing

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Off-target interactions in the CRISPR-Cas9 Machinery: mechanisms and outcomes

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