The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins
Abstract
Select members of the Wnt family of secreted glycoproteins have been implicated in inducing the myogenic determinant genes Pax3, MyoD and Myf5 during mammalian embryogenesis, but the mechanism of induction has not been defined. We describe an unexpected role for protein kinase A (PKA) signalling via CREB in this induction. Using a combination of in vitro explant assays, mutant analysis and gene delivery into mouse embryos cultured ex vivo, we demonstrate that adenylyl cyclase signalling via PKA and its target transcription factor CREB are required for Wnt-directed myogenic gene expression. Wnt proteins can also stimulate CREB-mediated transcription, providing evidence for a Wnt signalling pathway involving PKA and CREB. Our findings raise the possibility that PKA/CREB signalling may also contribute to other Wnt-regulated processes in embryonic patterning, stem cell renewal and cancer.
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“During myogenesis, Wnt signaling via PKA and the transcription factor CREB modulates gene transcription of muscle specific genes including MyoD and Myf5.111”
From Wnt signal transduction pathways (2008) · cites this paper as [111]
Citations
Cites
CREB: A Stimulus-Induced Transcription Factor Activated by A Diverse Array of Extracellular Signals
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Function and regulation of CREB family transcription factors in the nervous system
Lonze, B. E., & Ginty, D. D. (2002) · Neuron · doi:10.1016/s0896-6273(02)00828-0 · Cited by 2,127
Self-citationMyoD or Myf-5 is required for the formation of skeletal muscle
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The Promise and Perils of Wnt Signaling Through β-Catenin
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Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds
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- Manning, D. G proteins: Techniques of Analysis (Florida, CRC, 1999)
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Yang, Z., Ping, Y.-Q., Wang, M., Zhang, C., Zhou, S., Xi, Y.-T., Zhu, K., Zhang, Q., Song, Z.-C., Zhao, R.-J., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4677429
Metavinculin mediates differentiation cues into myogenic gene expressionPreprint
Murugan, A., Nanjegowda, N. B., & Jothi, M. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.21.578728
Saini, M., Jadhav, J., Cossu, G., & Mathew, S. J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.22.660860
Li, Y., Duan, D., Zhu, S., Liu, Z., Zhang, Q., & Wu, L. (2025) · Communications Biology · doi:10.1038/s42003-025-08718-0
Mantovani, C. S., Metzker-Pinto, T., Leite, I. B., Sampar, J. F., Fontoura, M. A., Jurberg, A. D., Nery, M. F., & Alvares, L. E. (2026) · Developmental Biology Advances · doi:10.1186/s13227-025-00256-4
Mécanismes moléculaires du contrôle de la masse musculaire sous l'action du β2-agoniste formotérol
Joassard, O. (2013) · theses.fr (ABES) · doi:10.70675/251c01fcz1479z415aza11cz70e69f50a189
Voie de signalisation WNT/ β-catenin et différenciation des cellules germinales chez les mammifères
Rolle, M. L. (2019) · theses.fr (ABES) · doi:10.70675/fe50a059z8f58z4591za5e0zd9c9260561e5
Alamandine/MrgD pathway modulates gut–bone marrow axis in ageing
Chittimalli, K., Rozario, H. E., Martinez, V., McAdams, Z. L., Adkins, S., Ericsson, A. C., & Jarajapu, Y. P. R. (2026) · British Journal of Pharmacology · doi:10.1111/bph.70656 · Cited by 1
Metadata
- Authors
- Chen, A. E., Ginty, D. D., & Fan, C.-M.
- Affiliations
- Carnegie Institution for Science (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Fan, Chen-Ming
- Venue
- Nature
- Published
- 2004-11-28
- Volume
- 433
- Issue
- 7023
- Pages
- 317-322
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2073269248
- Cited by
- 309
- References
- 27
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 10 · 2023: 8 · 2022: 9 · 2021: 8 · 2020: 12 · 2019: 13 · 2018: 7 · 2017: 15 · 2016: 6 · 2015: 14 · 2014: 12 · 2013: 15 · 2012: 17
- FWCI
- 8.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- Wnt signaling, protein kinase A, CREB, myogenesis, Pax3, MyoD, Myf5, adenylate cyclase, transcriptional regulation, embryonic patterning
- MeSH terms
- PAX3 Transcription Factor, Adenylyl Cyclases, Animals, Cell Line, DNA-Binding Proteins, Muscle Proteins, Muscles, Mutation, RNA, Messenger, Transcription Factors, Signal Transduction, Trans-Activators, Cyclic AMP Response Element-Binding Protein, MyoD Protein, Cyclic AMP-Dependent Protein Kinases, Mice, Knockout, Gene Expression Regulation, Developmental, Coculture Techniques, Somites, Muscle Development, Intercellular Signaling Peptides and Proteins, Embryo Culture Techniques, Wnt Proteins, Mice, Paired Box Transcription Factors, Myogenic Regulatory Factor 5
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