The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Transposition of hAT elements links transposable elements and V(D)J recombination
Abstract
Transposons are DNA sequences that encode functions that promote their movement to new locations in the genome. If unregulated, such movement could potentially insert additional DNA into genes, thereby disrupting gene expression and compromising an organism's viability. Transposable elements are classified by their transposition mechanisms and by the transposases that mediate their movement. The mechanism of movement of the eukaryotic hAT superfamily elements was previously unknown, but the divergent sequence of hAT transposases from other elements suggested that these elements might use a distinct mechanism. Here we have analysed transposition of the insect hAT element Hermes in vitro. Like other transposons, Hermes excises from DNA via double-strand breaks between the donor-site DNA and the transposon ends, and the newly exposed transposon ends join to the target DNA. Interestingly, the ends of the donor double-strand breaks form hairpin intermediates, as observed during V(D)J recombination, the process which underlies the combinatorial formation of antigen receptor genes. Significant similarities exist in the catalytic amino acids of Hermes transposase, the V(D)J recombinase RAG, and retroviral integrase superfamily transposases, thereby linking the movement of transposable elements and V(D)J recombination.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“This includes the distances between the second D and E residues, which are much longer in Transib transposons (206–214 aa) and RAG1 (253 aa) than in DDE TPases from other studied superfamilies (e.g., approximately 35-aa in Mariner/Tc1 [23], 2-aa in P [23], approximately 35-aa in Harbinger [24], with hAT as an exception (325-aa, [25]).”
From RAG1 Core and V(D)J Recombination Signal Sequences Were Derived from Transib Transposons (2005) · cites this paper as [25]
Citations
Cites
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.22.4673 · Cited by 64,617
Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
The sequence of the human genome
Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909
The PSIPRED protein structure prediction server
McGuffin, L. J., Bryson, K., & Jones, D. T. (2000) · Bioinformatics · doi:10.1093/bioinformatics/16.4.404 · Cited by 3,887
(2007) · Wiley eBooks · doi:10.1128/9781555817954 · Cited by 1,311
Ma, Y., Pannicke, U., Schwarz, K., & Lieber, M. R. (2002) · Cell · doi:10.1016/s0092-8674(02)00671-2 · Cited by 1,166
Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
Agrawal, A., Eastman, Q. M., & Schatz, D. G. (1998) · Nature · doi:10.1038/29457 · Cited by 811
Dyda, F., Hickman, A. B., Jenkins, T. M., Engelman, A., Craigie, R., & Davies, D. R. (1994) · Science · doi:10.1126/science.7801124 · Cited by 810 · cited 1×
“Structural analysis of the HIV integrase and Mu and Tn5 transposases has previously demonstrated that the highly conserved catalytic DDE acidic amino acids lie on a common structure, that is, an RNaseH-like fold, and that they are closely juxtaposed, as appropriate for formation of a single active sit”
BackgroundSelf-citationV(D)J Recombination: RAG Proteins, Repair Factors, and Regulation
Gellert, M. F. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.090501.150203 · Cited by 771
DNA Transposition by the RAG1 and RAG2 Proteins
Hiom, K., Melek, M., & Gellert, M. (1998) · Cell · doi:10.1016/s0092-8674(00)81587-1 · Cited by 534
Cited by
Sorted by citations
DNA transposons and the evolution of eukaryotic genomes.
Feschotte, C., & Pritham, E. J. (2007) · Annual Review of Genetics · doi:10.1146/annurev.genet.40.110405.090448 · Cited by 1,218
The Evolution of Adaptive Immune Systems
Cooper, M. D., & Alder, M. N. (2006) · Cell · doi:10.1016/j.cell.2006.02.001 · Cited by 875
Nowotny, M., Gaidamakov, S. A., Crouch, R. J., & Yang, W. (2005) · Cell · doi:10.1016/j.cell.2005.04.024 · Cited by 620
LINE-1 elements in structural variation and disease
Beck, C. R., Garcia-Perez, J. L., Badge, R. M., & Moran, J. V. (2011) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev-genom-082509-141802 · Cited by 615
RAG1 Core and V(D)J Recombination Signal Sequences Were Derived from Transib Transposons
Kapitonov, V. V., & Jurka, J. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030181 · Cited by 521
Metadata
- Authors
- Zhou, L., Mitra, R., Atkinson, P. W., Burgess Hickman, A., Dyda, F., & Craig, N. L.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of California, Riverside (US)
- National Institutes of Health (US)
- Corresponding author
- Craig, Nancy L.
- Venue
- Nature
- Published
- 2004-12
- Volume
- 432
- Issue
- 7020
- Pages
- 995-1001
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2071441005
- Cited by
- 224
- References
- 50
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 6 · 2017: 7 · 2016: 6 · 2015: 23 · 2014: 15 · 2013: 11 · 2012: 19
- FWCI
- 20.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- hAT transposons, transposition mechanism, V(D)J recombination, DNA double-strand breaks, transposon excision
- MeSH terms
- Animals, Base Sequence, Binding Sites, DNA, DNA Transposable Elements, Drosophila melanogaster, Nucleic Acid Conformation, Recombination, Genetic, Substrate Specificity, Gene Rearrangement, B-Lymphocyte, Homeodomain Proteins, Transposases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
