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Fast vesicle reloading and a large pool sustain high bandwidth transmission at a central synapse

Saviane, Chiara, Silver, R. Angus·2006·Nature
DOI 10.1038/nature04509
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Abstract

What limits the rate at which sensory information can be transmitted across synaptic connections in the brain? High-frequency signalling is restricted to brief bursts at many central excitatory synapses, whereas graded ribbon-type synapses can sustain release and transmit information at high rates. Here we investigate transmission at the cerebellar mossy fibre terminal, which can fire at over 200 Hz for sustained periods in vivo, yet makes few synaptic contacts onto individual granule cells. We show that connections between mossy fibres and granule cells can sustain high-frequency signalling at physiological temperature. We use fluctuation analysis and pharmacological block of desensitization to identify the quantal determinants of short-term plasticity and combine these with a short-term plasticity model and cumulative excitatory postsynaptic current analysis to quantify the determinants of sustained high-frequency transmission. We show that release is maintained at each release site by rapid reloading of release-ready vesicles from an unusually large releasable pool of vesicles (approximately 300 per site). Our results establish that sustained vesicular release at high rates is not restricted to graded ribbon-type synapses and that mossy fibres are well suited for transmitting broad-bandwidth rate-coded information to the input layer of the cerebellar cortex.

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Research works printed but not linked: 27

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Metadata

Authors
Saviane, C., & Silver, R. A.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2006-02
Volume
439
Issue
7079
Pages
983-987
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature04509
Cited by
241
References
3
Language
en
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Fast vesicle reloading and a large pool sustain high bandwidth transmission at a central synapse. https://app.cochat.ai/papers/10.1038/nature04509
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Saviane, C., & Silver, R. A. (2006). Fast vesicle reloading and a large pool sustain high bandwidth transmission at a central synapse. Nature. https://doi.org/10.1038/nature04509