A large pool of releasable vesicles in a cortical glutamatergic synapse
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To probe exocytosis at a cortical glutamatergic synapse, we made capacitance measurements in whole-cell recorded hippocampal mossy fiber terminals. Evaluation of different methods by using a morphology-based equivalent electrical model revealed that quantitative capacitance measurements are possible in this presynaptic structure. Voltage pulses leading to presynaptic Ca2+ inflow evoked large capacitance signals that showed saturation with increasing pulse duration. The mean peak capacitance increase was 100 fF, corresponding to a pool of approximately 1,400 releasable vesicles. Thus hippocampal mossy fiber synapses have a vesicular "maxipool." Large pool size and rapid vesicle recycling may underlie the uniquely large extent of activity-dependent plasticity in this synapse.
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Capacitance measurements from hippocampal mossy fiber terminals suggest that long depolarizing pulses release approximately 500-1,400 vesicles, corresponding to 17 to 48 vesicles per AZ.
“Capacitance measurements from hippocampal mossy fiber terminals suggest that long depolarizing pulses release approximately 500–1,400 vesicles, corresponding to 17 to 48 vesicles per AZ [33,86].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [86]
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- Authors
- Hallermann, S., Pawlu, C., Jonas, P., & Heckmann, M.
- Affiliations
- University of Freiburg (DE)
- Physiologisches Institut, Universität Freiburg, D-79104 Freiburg, Germany
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2003-06-18
- Volume
- 100
- Issue
- 15
- Pages
- 8975-8980
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC166423
- OpenAlex
- W2133521893
- Cited by
- 181
- References
- 60
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 8 · 2021: 14 · 2020: 7 · 2019: 13 · 2018: 9 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 8 · 2013: 4 · 2012: 4
- FWCI
- 3.35
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- exocytosis, glutamatergic synapses, hippocampal mossy fibers, synaptic vesicles, capacitance measurement, presynaptic calcium influx, vesicle recycling, activity-dependent plasticity, equivalent circuit model
- MeSH terms
- Animals, Electrophysiology, Endocytosis, Exocytosis, Models, Neurological, Synaptic Transmission, Rats, Wistar, Mossy Fibers, Hippocampal, Secretory Vesicles, Electric Capacitance, Rats, In Vitro Techniques
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