Non‐NMDA glutamate receptor occupancy and open probability at a rat cerebellar synapse with single and multiple release sites.
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1. Excitatory postsynaptic currents (EPSCs) were recorded under whole-cell voltage clamp from granule cells in slices of rat cerebellum. EPSCs from individual mossy fibre inputs were identified by their all-or-none appearance in response to a graded stimulus. Excitatory synaptic transmission was investigated at room temperature (approximately 24 degrees C) and at near-physiological temperature (approximately 34 degrees C) by analysing current fluctuations in the peak and decay of the non-N-methyl-D-aspartate (non-NMDA) component of EPSCs. 2. In a subset of synapses the mean EPSC amplitude remained unchanged as the probability of transmitter release was substantially lowered by raising the extracellular [Mg2+] and lowering [Ca2+]. These synapses were considered to have only one functional release site. Single-site synapses had small EPSCs (139 +/- 16 pS, n = 5, at 24 degrees C) with a large coefficient of variation (c.v. = 0.23 +/- 0.02, n = 5) and an amplitude distribution that was well fitted by a Gaussian distribution in four out of five cases. The EPSC latency had a unimodal distribution and its standard deviation had a temperature dependence with a temperature coefficient (Q10; range, 24-35 degrees C) of 2.4 +/- 0.4 (n = 4). 3. Peak-scaled non-stationary fluctuation analysis of single-site EPSCs indicated that the mean conductance of the underlying non-NMDA channels was 12 +/- 2 pS (n = 4) at 35 degrees C. Upper and lower limits for mean channel open probability (Po), cal…
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The estimation of N was not significantly affected by dendritic filtering in the paper's simulation experiments.
“The estimation of N was not significantly affected by the dendritic filtering in our simulation experiments38.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [38]
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- Authors
- Silver, R. A., Cull-Candy, S. G., & Takahashi, T.
- Affiliations
- University College London (GB)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 1996-07
- Volume
- 494
- Issue
- 1
- Pages
- 231-250
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1160626
- OpenAlex
- W2023459285
- Cited by
- 260
- References
- 48
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 12 · 2014: 7 · 2013: 6 · 2012: 10
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Keywords
- non-NMDA receptors, receptor occupancy, open probability, mossy fiber synapses, granule cells, cerebellum, temperature dependence, synaptic transmission, calcium dependence, mean-variance relationship, receptor desensitization
- MeSH terms
- Animals, Cerebellum, Membrane Potentials, Receptors, N-Methyl-D-Aspartate, Presynaptic Terminals, Patch-Clamp Techniques, Rats
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