The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
SAP-controlled T–B cell interactions underlie germinal centre formation
Open accessAbstract
Generation of long-term antibody-mediated immunity depends on the germinal centre reaction, which requires cooperation between antigen-specific T and B lymphocytes. In human X-linked lymphoproliferative disease and its gene-targeted mouse model, loss-of-function mutations in signalling lymphocyte activation molecule-associated protein (SAP, encoded by SH2D1a) cause a profound defect in germinal centre formation by an as yet unknown mechanism. Here, using two-photon intravital imaging, we show that SAP deficiency selectively impairs the ability of CD4(+) T cells to stably interact with cognate B cells but not antigen-presenting dendritic cells. This selective defect results in a failure of antigen-specific B cells to receive adequate levels of contact-dependent T-cell help to expand normally, despite Sap(-/-) T cells exhibiting the known characteristics of otherwise competent helper T cells. Furthermore, the lack of stable interactions with B cells renders Sap(-/-) T cells unable to be efficiently recruited to and retained in a nascent germinal centre to sustain the germinal centre reaction. These results offer an explanation for the germinal centre defect due to SAP deficiency and provide new insights into the bi-directional communication between cognate T and B cells in vivo.
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“A robust T follicular helper response sustains the germinal centre reaction34,35.”
From SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses (2021) · cites this paper as [34]
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T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases
Mempel, T. R., Henrickson, S. E., & von Andrian, U. H. (2004) · Nature · doi:10.1038/nature02238 · Cited by 1,720
Antigen-specific interaction between T and B cells
Lanzavecchia, A. (1985) · Nature · doi:10.1038/314537a0 · Cited by 1,416
Intraclonal generation of antibody mutants in germinal centres
Jacob, J., Kelsoe, G. H., Rajewsky, K., & Weiss, U. K. (1991) · Nature · doi:10.1038/354389a0 · Cited by 1,092
Sayós, J., Wu, C., Morra, M., Wang, N., Zhang, X., Allen, D., van Schaik, S., Notarangelo, L. D., Geha, R. S., Roncarolo, M., et al. (1998) · Nature · doi:10.1038/26683 · Cited by 940
Maturation of the immune response in germinal centers
Berek, C., Berger, A., & Apel, M. (1991) · Cell · doi:10.1016/0092-8674(91)90289-b · Cited by 914
Visualization of Specific B and T Lymphocyte Interactions in the Lymph Node
Garside, P., Ingulli, E. G., Merica, R. R., Johnson, J. G., Noelle, R. J., & Jenkins, M. K. (1998) · Science · doi:10.1126/science.281.5373.96 · Cited by 774
Coffey, A. J., Brooksbank, R. A., Brandau, O., Oohashi, T., Howell, G. R., Bye, J. M., Cahn, A. P., Durham, J., Heath, P. D., Wray, P. W., et al. (1998) · Nature Genetics · doi:10.1038/2424 · Cited by 752
Liu, Y., Zhang, J., Lane, P. J. L., Chan, E. Y. ‐., & MacLennan, I. C. M. (1991) · European Journal of Immunology · doi:10.1002/eji.1830211209 · Cited by 736
Chtanova, T., Tangye, S. G., Newton, R., Frank, N., Hodge, M. R., Rolph, M. S., & Mackay, C. R. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.1.68 · Cited by 722
Dynamic Imaging of T Cell-Dendritic Cell Interactions in Lymph Nodes
Stoll, S., Delon, J., Brotz, T. M., & Germain, R. N. (2002) · Science · doi:10.1126/science.1071065 · Cited by 718
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Follicular Helper CD4 T Cells (T FH )
Crotty, S. (2011) · Annual Review of Immunology · doi:10.1146/annurev-immunol-031210-101400 · Cited by 2,738
Victora, G. D., & Nussenzweig, M. C. (2012) · Annual Review of Immunology · doi:10.1146/annurev-immunol-020711-075032 · Cited by 1,878
T follicular helper cell differentiation, function, and roles in disease
Crotty, S. (2014) · Immunity · doi:10.1016/j.immuni.2014.10.004 · Cited by 1,847
Foxp3+ follicular regulatory T cells control the germinal center response
Linterman, M. A., Pierson, W., Lee, S. K., Kallies, A., Kawamoto, S., Rayner, T. F., Srivastava, M., Divekar, D. P., Beaton, L., Hogan, J. J., et al. (2011) · Nature Medicine · doi:10.1038/nm.2425 · Cited by 1,292
Vinuesa, C. G., Linterman, M. A., Yu, D., & MacLennan, I. C. M. (2016) · Annual Review of Immunology · doi:10.1146/annurev-immunol-041015-055605 · Cited by 1,141
Metadata
- Authors
- Qi, H., Cannons, J. L., Klauschen, F., Schwartzberg, P. L., & Germain, R. N.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Schwartzberg, Pamela L.
- Germain, Ronald N.
- Venue
- Nature
- Published
- 2008-10
- Volume
- 455
- Issue
- 7214
- Pages
- 764-769
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC2652134
- OpenAlex
- W1990297263
- Cited by
- 637
- References
- 45
- Citations by year
- 2026: 10 · 2025: 16 · 2024: 16 · 2023: 15 · 2022: 42 · 2021: 39 · 2020: 30 · 2019: 35 · 2018: 44 · 2017: 37 · 2016: 44 · 2015: 35 · 2014: 47 · 2013: 53 · 2012: 48
- FWCI
- 17.45
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (Z01AI000545)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunodeficiency and Autoimmune Disorders
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- SH2D1A, X-linked lymphoproliferative disease, germinal center formation, T cell-B cell interaction, CD4+ T cell help, B cell activation, affinity maturation, intravital imaging
- MeSH terms
- Signaling Lymphocytic Activation Molecule Associated Protein, Animals, B-Lymphocytes, Cell Adhesion, Cell Communication, Cells, Cultured, Chimera, Dendritic Cells, Lymphocyte Activation, CD4-Positive T-Lymphocytes, Germinal Center, Intracellular Signaling Peptides and Proteins, Mice
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