The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Structure of the outer membrane complex of a type IV secretion system
Open accessAbstract
Type IV secretion systems are secretion nanomachines spanning the two membranes of Gram-negative bacteria. Three proteins, VirB7, VirB9 and VirB10, assemble into a 1.05 megadalton (MDa) core spanning the inner and outer membranes. This core consists of 14 copies of each of the proteins and forms two layers, the I and O layers, inserting in the inner and outer membrane, respectively. Here we present the crystal structure of a ∼0.6 MDa outer-membrane complex containing the entire O layer. This structure is the largest determined for an outer-membrane channel and is unprecedented in being composed of three proteins. Unexpectedly, this structure identifies VirB10 as the outer-membrane channel with a unique hydrophobic double-helical transmembrane region. This structure establishes VirB10 as the only known protein crossing both membranes of Gram-negative bacteria. Comparison of the cryo-electron microscopy (cryo-EM) and crystallographic structures points to conformational changes regulating channel opening and closing.
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McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., & Read, R. J. (2007) · Journal of Applied Crystallography · doi:10.1107/s0021889807021206 · Cited by 20,612
PHENIX : building new software for automated crystallographic structure determination
Adams, P. D., Grosse-Kunstleve, R. W., Hung, L.-W., Ioerger, T. R., McCoy, A. J., Moriarty, N. W., Read, R. J., Sacchettini, J. C., Sauter, N. K., & Terwilliger, T. C. (2002) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444902016657 · Cited by 4,361
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Self-citationAgrobacterium-Mediated Plant Transformation: Biology and Applications
Hwang, H.-H., Yu, M., & Lai, E.-M. (2017) · The Arabidopsis Book · doi:10.1199/tab.0186 · Cited by 312
A comprehensive guide to pilus biogenesis in Gram-negative bacteria
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Metadata
- Authors
- Chandran, V., Fronzes, R., Duquerroy, S., Cronin, N., Navaza, J., & Waksman, G.
- Venue
- Nature
- Published
- 2009-11-29
- Volume
- 462
- Issue
- 7276
- Pages
- 1011-1015
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2043126144
- Cited by
- 303
- References
- 3
- FWCI
- 14.08
- Type
- journal article
- Language
- en
- Open access
- green
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