The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Production of p53 gene knockout rats by homologous recombination in embryonic stem cells
Open accessAbstract
The use of homologous recombination to modify genes in embryonic stem (ES) cells provides a powerful means to elucidate gene function and create disease models. Application of this technology to engineer genes in rats has not previously been possible because of the absence of germline-competent ES cells in this species. We have recently established authentic rat ES cells. Here we report the generation of gene knockout rats using the ES-cell-based gene targeting technology. We designed a targeting vector to disrupt the tumour suppressor gene p53 (also known as Tp53) in rat ES cells by means of homologous recombination. p53 gene-targeted rat ES cells can be routinely generated. Furthermore, the p53 gene-targeted mutation in the rat ES-cell genome can transmit through the germ line via ES-cell rat chimaeras to create p53 gene knockout rats. The rat is the most widely used animal model in biological research. The establishment of gene targeting technology in rat ES cells, in combination with advances in genomics and the vast amount of research data on physiology and pharmacology in this species, now provide a powerful new platform for the study of human disease.
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The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442
Self-citationProgress and prospects in rat genetics: a community view
Aitman, T. J., Critser, J. K., Cuppen, E., Dominiczak, A., Fernandez-Suarez, X. M., Flint, J., Gauguier, D., Geurts, A. M., Gould, M., Harris, P. C., et al. (2008) · Nature Genetics · doi:10.1038/ng.147 · Cited by 291
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Carroll, D. (2011) · Genetics · doi:10.1534/genetics.111.131433 · Cited by 1,076
Li, W., Teng, F., Li, T., & Zhou, Q. (2013) · Nature Biotechnology · doi:10.1038/nbt.2652 · Cited by 425
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Kaneko, T., Sakuma, T., Yamamoto, T., & Mashimo, T. (2014) · Scientific Reports · doi:10.1038/srep06382 · Cited by 204
From engineering to editing the rat genome
Meek, S., Mashimo, T., & Burdon, T. (2017) · Mammalian Genome · doi:10.1007/s00335-017-9705-8 · Cited by 63
Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models
Chenouard, V., Remy, S., Tesson, L., Ménoret, S., Ouisse, L.-H., Cherifi, Y., & Anegon, I. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.615491 · Cited by 57
Metadata
- Authors
- Tong, C., Li, P., Wu, N. L., Yan, Y., & Ying, Q.-L.
- Venue
- Nature
- Published
- 2010-08-11
- Volume
- 467
- Issue
- 7312
- Pages
- 211-213
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2054857432
- Cited by
- 267
- References
- 2
- FWCI
- 21.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Tong, C., Li, P., Wu, N. L., Yan, Y., & Ying, Q.-L. (2010). Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature. https://doi.org/10.1038/nature09368
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