Genome Engineering With Zinc-Finger Nucleases
Open accessAbstract
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been made to genome editing in many different organisms and situations. Considerable progress has been made in methods for deriving zinc-finger sets for new genomic targets, but approaches to design and selection are still being perfected. An issue that needs more attention is the extent to which available mechanisms of double-strand break repair limit the scope and utility of ZFN-initiated events. The bright prospects for future applications of ZFNs, including human gene therapy, are discussed.
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ZNF creates targeted double-strand breaks, commonly producing frameshift or nonsense mutations that create null alleles.
“The ZNF techniques preceded CRSISPR but work similarly in that the ZNF creates targeted double stranded breaks in the sequence of interest with a common outcome being the generation of a frameshift or nonsense mutation creating a null allele (Carroll, 2011).”
From Rodent Genetic Models of Ah Receptor Signaling (2021) · cites this paper as Carroll, 2011
The Abstract and “Stimulating Gene Targeting With Double-Strand Breaks” describe targeted ZFN-induced breaks and mutations produced when those breaks are repaired. The latter section identifies deletions, insertions, and substitutions, but does not say that frameshift or nonsense mutations commonly result or that they create null alleles. No page numbers were available in our copy. (LLM, conf=0.84)
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- Authors
- Carroll, D.
- Affiliations
- University of Utah (US)
- Corresponding author
- Carroll, Dana
- ORCID
- Carroll, Dana
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-08-01
- Volume
- 188
- Issue
- 4
- Pages
- 773-782
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC3176093
- OpenAlex
- W2123196886
- Cited by
- 1,076
- References
- 5
- Citations by year
- 2026: 41 · 2025: 86 · 2024: 95 · 2023: 89 · 2022: 74 · 2021: 79 · 2020: 83 · 2019: 59 · 2018: 60 · 2017: 67 · 2016: 60 · 2015: 68 · 2014: 73 · 2013: 84 · 2012: 51
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM58504, GM078571, R01GM078571, R01GM058504)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- zinc finger nucleases, genome editing, targeted mutagenesis, targeted gene replacement, DNA double-strand break repair, human gene therapy, gene targeting
- MeSH terms
- Animals, Endonucleases, Genetic Engineering, Humans, Substrate Specificity, Zinc Fingers, Genome, Gene Targeting, DNA Breaks, Double-Stranded
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