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Functional mapping of single spines in cortical neurons in vivo.
Abstract
The individual functional properties and spatial arrangement of afferent synaptic inputs on dendrites have a critical role in the processing of information by neurons in the mammalian brain. Although recent work has identified visually-evoked local dendritic calcium signals in the rodent visual cortex, sensory-evoked signalling on the level of dendritic spines, corresponding to individual afferent excitatory synapses, remains unexplored. Here we used a new variant of high-resolution two-photon imaging to detect sensory-evoked calcium transients in single dendritic spines of mouse cortical neurons in vivo. Calcium signals evoked by sound stimulation required the activation of NMDA (N-methyl-D-aspartate) receptors. Active spines are widely distributed on basal and apical dendrites and pure-tone stimulation at different frequencies revealed both narrowly and widely tuned spines. Notably, spines tuned for different frequencies were highly interspersed on the same dendrites: even neighbouring spines were mostly tuned to different frequencies. Thus, our results demonstrate that NMDA-receptor-dependent single-spine synaptic inputs to the same dendrite are highly heterogeneous. Furthermore, our study opens the way for in vivo mapping of functionally defined afferent sensory inputs with single-synapse resolution.
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“Combining in vivo whole-cell patch recording and single spine calcium imaging, a recent work has shown that spines tuned for different peak frequencies are interspersed along dendrites of pyramidal neurons in the mouse auditory cortex (Chen et al., 2011).”
From Spatiotemporal dynamics of dendritic spines in the living brain (2014) · cites this paper as Chen et al., 2011
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Metadata
- Authors
- Chen, X., Leischner, U., Rochefort, N. L., Nelken, I., & Konnerth, A.
- Affiliations
- Center for Integrated Protein Science Munich (DE)
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Konnerth, Arthur
- Venue
- Nature
- Published
- 2011-06-26
- Volume
- 475
- Issue
- 7357
- Pages
- 501-505
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1985450720
- Cited by
- 395
- References
- 43
- Citations by year
- 2026: 16 · 2025: 19 · 2024: 26 · 2023: 20 · 2022: 19 · 2021: 23 · 2020: 21 · 2019: 31 · 2018: 24 · 2017: 29 · 2016: 20 · 2015: 30 · 2014: 32 · 2013: 37 · 2012: 39
- FWCI
- 15.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- dendritic spines, auditory cortex, NMDA receptor, frequency tuning, dendritic integration, two-photon calcium imaging, mouse auditory cortex, in vivo imaging
- MeSH terms
- Acoustic Stimulation, Animals, Brain Mapping, Image Processing, Computer-Assisted, Mice, Inbred C57BL, Visual Cortex, Calcium Signaling, Microscopy, Fluorescence, Multiphoton, Dendritic Spines, Mice
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