Spine-Neck Geometry Determines NMDA Receptor-Dependent Ca2+ Signaling in Dendrites
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Increases in cytosolic Ca2+ concentration ([Ca2+]i) mediated by NMDA-sensitive glutamate receptors (NMDARs) are important for synaptic plasticity. We studied a wide variety of dendritic spines on rat CA1 pyramidal neurons in acute hippocampal slices. Two-photon uncaging and Ca2+ imaging revealed that NMDAR-mediated currents increased with spine-head volume and that even the smallest spines contained a significant number of NMDARs. The fate of Ca2+ that entered spine heads through NMDARs was governed by the shape (length and radius) of the spine neck. Larger spines had necks that permitted greater efflux of Ca2+ into the dendritic shaft, whereas smaller spines manifested a larger increase in [Ca2+]i within the spine compartment as a result of a smaller Ca2+ flux through the neck. Spine-neck geometry is thus an important determinant of spine Ca2+ signaling, allowing small spines to be the preferential sites for isolated induction of long-term potentiation.
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Synaptic stimulation alone induces a marked increase in intracellular calcium concentration (>10 μM) of spines via NMDA receptors.
“In contrast, synaptic stimulation alone was not sufficient to trigger BDNF secretion (fig. S7), even though it induces a marked increase in the intracellular calcium concentration {[Ca2+]i} (>10 μM) of spines via NMDA receptors (21).”
From Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines (2008) · cites this paper as [21]
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- Authors
- Noguchi, J., Matsuzaki, M., Ellis-Davies, G. C. R., & Kasai, H.
- Affiliations
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Corresponding author
- Kasai, Haruo
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2005-05
- Volume
- 46
- Issue
- 4
- Pages
- 609-622
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC4151245
- OpenAlex
- W1983284219
- Cited by
- 419
- References
- 11
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 13 · 2023: 17 · 2022: 14 · 2021: 18 · 2020: 20 · 2019: 25 · 2018: 14 · 2017: 18 · 2016: 13 · 2015: 21 · 2014: 25 · 2013: 15 · 2012: 29
- FWCI
- 9.88
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2999 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- SOKENDAI (Graduate University for Advanced Studies) (submittedVersion, cc-by-nc-nd)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- McKnight Endowment Fund for Neuroscience
- Takeda Science Foundation
- National Science Foundation
- National Institutes of Health
- Human Frontier Science Program
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Keywords
- dendritic spines, NMDA receptor, calcium signaling, CA1 pyramidal neurons, hippocampus, long-term potentiation, synaptic plasticity, calcium imaging, two-photon uncaging
- MeSH terms
- Animals, Animals, Newborn, Calcium, Diagnostic Imaging, Dose-Response Relationship, Radiation, Glutamates, Hippocampus, Indoles, Lasers, Membrane Potentials, Models, Neurological, Synaptic Transmission, Valine, Receptors, N-Methyl-D-Aspartate, N-Methylaspartate, Rats, Sprague-Dawley, Pyramidal Cells, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Patch-Clamp Techniques, Calcium Signaling, Dendritic Spines, Rats, In Vitro Techniques
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