Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem
Open accessAbstract
Background Plants produce leaf and flower primordia from a specialized tissue called the shoot apical meristem (SAM). Genetic studies have identified a large number of genes that affect various aspects of primordium development including positioning, growth, and differentiation. So far, however, a detailed understanding of the spatio-temporal sequence of events leading to primordium development has not been established. Results We use confocal imaging of green fluorescent protein (GFP) reporter genes in living plants to monitor the expression patterns of multiple proteins and genes involved in flower primordial developmental processes. By monitoring the expression and polarity of PINFORMED1 (PIN1), the auxin efflux facilitator, and the expression of the auxin-responsive reporter DR5, we reveal stereotypical PIN1 polarity changes which, together with auxin induction experiments, suggest that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. Imaging of multiple GFP-protein fusions shows that these dynamics also correlate with the specification of primordial boundary domains, organ polarity axes, and the sites of floral meristem initiation. Conclusions These results provide new insight into auxin transport dynamics during primordial positioning and suggest a role for auxin transport in influencing primordial cell type.
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In the inflorescence meristem, polar auxin transport via PIN1 leads to local auxin maxima at positions where new flower primordia will form.
“In the inflorescence meristem, polar auxin transport via PIN1 leads to local auxin maxima at positions where new flower primordia will form (Heisler et al., 2005).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Heisler et al., 2005
The boundary between the floral meristem and the inflorescence meristem constitutes a low-auxin environment.
“Therefore, the boundary between the floral meristem and the inflorescence meristem also constitutes a lowauxin environment (Heisler et al., 2005;Vernoux et al., 2011).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Heisler et al., 2005
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- Authors
- Heisler, M. G., Ohno, C., Das, P., Sieber, P., Reddy, G. V., Long, J. A., & Meyerowitz, E. M.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- Meyerowitz, Elliot M.
- ORCID
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2005-11
- Volume
- 15
- Issue
- 21
- Pages
- 1899-1911
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2089118630
- Cited by
- 1,189
- References
- 7
- Citations by year
- 2026: 35 · 2025: 52 · 2024: 48 · 2023: 53 · 2022: 65 · 2021: 54 · 2020: 75 · 2019: 60 · 2018: 51 · 2017: 72 · 2016: 40 · 2015: 66 · 2014: 77 · 2013: 60 · 2012: 68
- FWCI
- 48.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2815 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- National Science Foundation (FIBR 0330786)
- U.S. Department of Energy (DOE FG02-88ER13873)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- auxin transport, auxin efflux, Pin1, shoot apical meristem, inflorescence meristem, DR5 reporter, organ polarity, live imaging, Arabidopsis thaliana
- MeSH terms
- Indoleacetic Acids, Models, Biological, Arabidopsis, DNA Primers, Homeodomain Proteins, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Meristem, Microscopy, Confocal, Membrane Transport Proteins, Arabidopsis Proteins, Flowers, Green Fluorescent Proteins
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