The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Genetic dissection of the circuit for hand dexterity in primates
Abstract
It is generally accepted that the direct connection from the motor cortex to spinal motor neurons is responsible for dexterous hand movements in primates. However, the role of the 'phylogenetically older' indirect pathways from the motor cortex to motor neurons, mediated by spinal interneurons, remains elusive. Here we used a novel double-infection technique to interrupt the transmission through the propriospinal neurons (PNs), which act as a relay of the indirect pathway in macaque monkeys (Macaca fuscata and Macaca mulatta). The PNs were double infected by injection of a highly efficient retrograde gene-transfer vector into their target area and subsequent injection of adeno-associated viral vector at the location of cell somata. This method enabled reversible expression of green fluorescent protein (GFP)-tagged tetanus neurotoxin, thereby permitting the selective and temporal blockade of the motor cortex–PN–motor neuron pathway. This treatment impaired reach and grasp movements, revealing a critical role for the PN-mediated pathway in the control of hand dexterity. Anti-GFP immunohistochemistry visualized the cell bodies and axonal trajectories of the blocked PNs, which confirmed their anatomical connection to motor neurons. This pathway-selective and reversible technique for blocking neural transmission does not depend on cell-specific promoters or transgenic techniques, and is a new and powerful tool for functional dissection in system-level neuroscience studies.
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“Recent studies successfully applied V16 to control transgene expression in mice [85, 86] and non-human primates [62, 87].”
From Tet-On Systems For Doxycycline-inducible Gene Expression (2016) · cites this paper as [87]
Citations
Cites
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Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors
Lois, C., Hong, E. J., Pease, S., Brown, E. J., & Baltimore, D. (2002) · Science · doi:10.1126/science.1067081 · Cited by 1,992
Optogenetics in neural systems
Yizhar, O., Fenno, L. E., Davidson, T. J., Mogri, M., & Deisseroth, K. (2011) · Neuron · doi:10.1016/j.neuron.2011.06.004 · Cited by 1,881
Descending Pathways in Motor Control
Lemon, R. N. (2008) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.31.060407.125547 · Cited by 1,599
THE FUNCTIONAL ORGANIZATION OF THE MOTOR SYSTEM IN THE MONKEY
Lawrence, D. G., & Kuypers, H. G. J. M. (1968) · Brain · doi:10.1093/brain/91.1.1 · Cited by 1,301
Transgenic inhibition of synaptic transmission reveals role of CA3 output in hippocampal learning
Nakashiba, T., Young, J. Z., McHugh, T. J., Buhl, D. L., & Tonegawa, S. (2008) · Science · doi:10.1126/science.1151120 · Cited by 492
Han, X., Chow, B. Y., Zhou, H., Klapoetke, N. C., Chuong, A. S., Rajimehr, R., Yang, A., Baratta, M. V., Winkle, J. A., Desimone, R., et al. (2011) · Frontiers in Systems Neuroscience · doi:10.3389/fnsys.2011.00018 · Cited by 490
An optogenetic toolbox designed for primates
Diester, I., Kaufman, M. T., Mogri, M., Pashaie, R., Goo, W., Yizhar, O., Ramakrishnan, C., Deisseroth, K., & Shenoy, K. V. (2011) · Nature Neuroscience · doi:10.1038/nn.2749 · Cited by 443
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Viral-genetic tracing of the input-output organization of a central noradrenaline circuit
Schwarz, L. A., Miyamichi, K., Gao, X. J., Beier, K. T., Weissbourd, B., DeLoach, K. E., Ren, J., Ibanes, S., Malenka, R. C., Kremer, E. J., et al. (2015) · Nature · doi:10.1038/nature14600 · Cited by 744
Spinal cord repair: advances in biology and technology
Courtine, G., & Sofroniew, M. V. (2019) · Nature Medicine · doi:10.1038/s41591-019-0475-6 · Cited by 508
Rizzolatti, G., Cattaneo, L., Fabbri‐Destro, M., & Rozzi, S. (2014) · Physiological Reviews · doi:10.1152/physrev.00009.2013 · Cited by 523
Tet-On Systems For Doxycycline-inducible Gene Expression
T. Das, A., Tenenbaum, L., & Berkhout, B. (2016) · Current Gene Therapy · doi:10.2174/1566523216666160524144041 · Cited by 434
“Recent studies successfully applied V16 to control transgene expression in mice [85, 86] and non-human primates [62, 87].”
BackgroundSkilled reaching relies on a V2a propriospinal internal copy circuit
Azim, E., Jiang, J., Alstermark, B., & Jessell, T. M. (2014) · Nature · doi:10.1038/nature13021 · Cited by 355
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Metadata
- Authors
- Kinoshita, M., Matsui, R., Kato, S., Hasegawa, T., Kasahara, H., Isa, K., Watakabe, A., Yamamori, T., Nishimura, Y., Alstermark, B., Watanabe, D., Kobayashi, K., & Isa, T.
- Affiliations
- National Institute for Physiological Sciences (JP)
- Kyoto University (JP)
- Fukushima Medical University (JP)
- National Institute for Basic Biology (JP)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Umeå University (SE)
- Corresponding author
- Isa, Tadashi
- ORCID
- Venue
- Nature
- Published
- 2012-06-17
- Volume
- 487
- Issue
- 7406
- Pages
- 235-238
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2052129348
- Cited by
- 259
- References
- 34
- Citations by year
- 2026: 7 · 2025: 18 · 2024: 9 · 2023: 11 · 2022: 18 · 2021: 26 · 2020: 15 · 2019: 31 · 2018: 17 · 2017: 16 · 2016: 20 · 2015: 26 · 2014: 20 · 2013: 20 · 2012: 5
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Vetenskapsrådet
- Japan Society for the Promotion of Science (22123009, 24220009, 20240030)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Motor Control and Adaptation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- manual dexterity, propriospinal neurons, motor cortex, spinal interneurons, tetanus neurotoxin, viral vectors, adeno-associated virus, synaptic transmission blockade, macaque
- MeSH terms
- Dependovirus, Animals, Hand, Macaca, Metalloendopeptidases, Motor Cortex, Motor Neurons, Synaptic Transmission, Neurosciences, Tetanus Toxin, Green Fluorescent Proteins
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