Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
Abstract
The zebrafish is firmly established as a genetic model for the study of vertebrate blood development. Here we have characterized the blood-forming system of adult zebrafish. Each major blood lineage can be isolated by flow cytometry, and with these lineal profiles, defects in zebrafish blood mutants can be quantified. We developed hematopoietic cell transplantation to study cell autonomy of mutant gene function and to establish a hematopoietic stem cell assay. Hematopoietic cell transplantation can rescue multilineage hematopoiesis in embryonic lethal gata1-/- mutants for over 6 months. Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis.
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The transplantable hematopoietic stem/progenitor cell marrow population resides within the adult kidney and can be isolated using flow cytometric analysis of forward and side scatter.
“Prior work from our laboratory has demonstrated that the transplantable hematopoietic stem/progenitor (HSPC) marrow population resides within the adult kidney, and can be isolated using flow cytometric analysis of forward and side scatter”
From Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis (2008) · cites this paper as Traver et al., 2003
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- Authors
- Traver, D., Paw, B. H., Poss, K. D., Penberthy, W. T., Lin, S., & Zon, L. I.
- Affiliations
- Boston Children's Hospital (US)
- Duke University (US)
- University of California, Los Angeles (US)
- Corresponding author
- Zon, Leonard I
- Venue
- Nature Immunology
- Published
- 2003-11-09
- Volume
- 4
- Issue
- 12
- Pages
- 1238-1246
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W2112528263
- Cited by
- 800
- References
- 4
- Citations by year
- 2026: 37 · 2025: 42 · 2024: 33 · 2023: 32 · 2022: 41 · 2021: 44 · 2020: 49 · 2019: 43 · 2018: 33 · 2017: 40 · 2016: 46 · 2015: 42 · 2014: 37 · 2013: 38 · 2012: 53
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- zebrafish, hematopoiesis, hematopoietic stem cell transplantation, multilineage engraftment, hematopoietic stem cells, cell autonomy, in vivo imaging, blood development
- MeSH terms
- Animals, Blood Cells, Blood Physiological Phenomena, Flow Cytometry, Hematopoietic Stem Cells, Hematopoietic System, Kidney, Mutation, Spleen, Zebrafish, Hematopoietic Stem Cell Transplantation, Cell Lineage, Animals, Genetically Modified
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