Cited by 351Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionAlgal biology and biofuel production

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Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform

Enquist-Newman, Maria, Faust, Ann Marie E., Bravo, Daniel D., Santos, Christine Nicole S., Raisner, Ryan M., Hanel, Arthur, Sarvabhowman, Preethi, Le, Chi, Regitsky, Drew D., Cooper, Susan R., Peereboom, Lars, Clark, Alana, Martinez, Yessica, Goldsmith, Joshua, Cho, Min Y., Donohoue, Paul D., Luo, Lily, Lamberson, Brigit, Tamrakar, Pramila, Kim, Edward J., Villari, Jeffrey L., Gill, Avinash, Tripathi, Shital A., Karamchedu, Padma, Paredes, Carlos J., Rajgarhia, Vineet, Kotlar, Hans Kristian, Bailey, Richard B., Miller, Dennis J., Ohler, Nicholas L., Swimmer, Candace, Yoshikuni, Yasuo·2014·Nature

DOI 10.1038/nature12771

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Abstract

The increasing demands placed on natural resources for fuel and food production require that we explore the use of efficient, sustainable feedstocks such as brown macroalgae. The full potential of brown macroalgae as feedstocks for commercial-scale fuel ethanol production, however, requires extensive re-engineering of the alginate and mannitol catabolic pathways in the standard industrial microbe Saccharomyces cerevisiae. Here we present the discovery of an alginate monomer (4-deoxy-L-erythro-5-hexoseulose uronate, or DEHU) transporter from the alginolytic eukaryote Asteromyces cruciatus. The genomic integration and overexpression of the gene encoding this transporter, together with the necessary bacterial alginate and deregulated native mannitol catabolism genes, conferred the ability of an S. cerevisiae strain to efficiently metabolize DEHU and mannitol. When this platform was further adapted to grow on mannitol and DEHU under anaerobic conditions, it was capable of ethanol fermentation from mannitol and DEHU, achieving titres of 4.6% (v/v) (36.2 g l(-1)) and yields up to 83% of the maximum theoretical yield from consumed sugars. These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks.

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Authors: Enquist-Newman, M., Faust, A. M. E., Bravo, D. D., Santos, C. N. S., Raisner, R. M., Hanel, A., Sarvabhowman, P., Le, C., Regitsky, D. D., Cooper, S. R., Peereboom, L., Clark, A., Martinez, Y., Goldsmith, J., Cho, M. Y., Donohoue, P. D., Luo, L., Lamberson, B., Tamrakar, P., Kim, E. J., Villari, J. L., Gill, A., Tripathi, S. A., Karamchedu, P., Paredes, C. J., Rajgarhia, V., Kotlar, H. K., Bailey, R. B., Miller, D. J., Ohler, N. L., Swimmer, C., & Yoshikuni, Y.
Affiliations: Manus Bio (United States) (US)
Michigan State University (US)
Equinor (Norway) (NO)
Corresponding author: Swimmer, Candace
Yoshikuni, Yasuo
ORCID: Faust, Ann Marie E.
Bravo, Daniel D.
Santos, Christine Nicole S.
Raisner, Ryan M.
Cooper, Susan R.
Peereboom, Lars
Donohoue, Paul D.
Gill, Avinash
Paredes, Carlos J.
Miller, Dennis J.
Yoshikuni, Yasuo
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2013-12-01
Volume: 505
Issue: 7482
Pages: 239-243
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature12771
OpenAlex: W2018440937
Cited by: 351
References: 30
Citations by year: 2026: 10 · 2025: 15 · 2024: 16 · 2023: 25 · 2022: 28 · 2021: 35 · 2020: 29 · 2019: 35 · 2018: 28 · 2017: 22 · 2016: 43 · 2015: 40 · 2014: 25
FWCI: 16.11
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords: brown macroalgae, alcoholic fermentation, Saccharomyces cerevisiae, biofuel feedstock, renewable chemicals, glucan, yeast strain engineering, anaerobic fermentation, metabolic engineering, chromosomal integration, overexpression
MeSH terms: Ethanol, Phaeophyceae, Alginates, Anaerobiosis, Ascomycota, Biotechnology, Carrier Proteins, Fermentation, Genetic Complementation Test, Genetic Engineering, Hexuronic Acids, Mannitol, Quinic Acid, Saccharomyces cerevisiae, Seaweed, Uronic Acids, Reproducibility of Results, Evolution, Molecular, Glucuronic Acid, Carbohydrate Metabolism, Biofuels

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Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform

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