The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Endosomes are specialized platforms for bacterial sensing and NOD2 signalling
Abstract
The detection of microbial pathogens involves the recognition of conserved microbial components by host cell sensors such as Toll-like receptors (TLRs) and NOD-like receptors (NLRs). TLRs are membrane receptors that survey the extracellular environment for microbial infections, whereas NLRs are cytosolic complexes that detect microbial products that reach the cytosol. Upon detection, both sensor classes trigger innate inflammatory responses and allow the engagement of adaptive immunity. Endo-lysosomes are the entry sites for a variety of pathogens, and therefore the sites at which the immune system first senses their presence. Pathogens internalized by endocytosis are well known to activate TLRs 3 and 7-9 that are localized to endocytic compartments and detect ligands present in the endosomal lumen. Internalized pathogens also activate sensors in the cytosol such as NOD1 and NOD2 (ref. 2), indicating that endosomes also provide for the translocation of bacterial components across the endosomal membrane. Despite the fact that NOD2 is well understood to have a key role in regulating innate immune responses and that mutations at the NOD2 locus are a common risk factor in inflammatory bowel disease and possibly other chronic inflammatory states, little is known about how its ligands escape from endosomes. Here we show that two endo-lysosomal peptide transporters, SLC15A3 and SLC15A4, are preferentially expressed by dendritic cells, especially after TLR stimulation. The transporters mediate the egress of bacterially derived components, such as the NOD2 cognate ligand muramyl dipeptide (MDP), and are selectively required for NOD2 responses to endosomally derived MDP. Enhanced expression of the transporters also generates endosomal membrane tubules characteristic of dendritic cells, which further enhanced the NOD2-dependent response to MDP. Finally, sensing required the recruitment of NOD2 and its effector kinase RIPK2 (refs 8, 9) to the endosomal membrane, possibly by forming a complex with SLC15A3 or SLC15A4. Thus, dendritic cell endosomes are specialized platforms for both the lumenal and cytosolic sensing of pathogens.
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“Previously, oligopeptide transporters encoded by SLC15A3 and SLC15A4 were reported to be responsible for transporting small peptidoglycan (PGN) fragments into macrophages (Nakamura et al., 2014).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Nakamura et al., 2014
In the citing paper
“It was previously reported that oligopeptide transporters encoded by SLC15A3 and SLC15A4 are responsible for transporting small PGN fragments into macrophages (Nakamura et al., 2014).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Nakamura et al., 2014
Citations
Cites
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A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
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Nod2 Is a General Sensor of Peptidoglycan through Muramyl Dipeptide (MDP) Detection
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ACIDIFICATION OF THE ENDOCYTIC AND EXOCYTIC PATHWAYS
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Self-citationSignals for Sorting of Transmembrane Proteins to Endosomes and Lysosomes
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Host Recognition of Bacterial Muramyl Dipeptide Mediated through NOD2
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Nod1 Detects a Unique Muropeptide from Gram-Negative Bacterial Peptidoglycan
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Metadata
- Authors
- Nakamura, N., Lill, J. R., Phung, Q., Jiang, Z., Bakalarski, C., de Mazière, A., Klumperman, J., Schlatter, M., Delamarre, L., & Mellman, I.
- Affiliations
- Genentech (US)
- University Medical Center Utrecht (NL)
- Corresponding author
- Mellman, Ira
- ORCID
- Venue
- Nature
- Published
- 2014-03-30
- Volume
- 509
- Issue
- 7499
- Pages
- 240-244
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2031113436
- Cited by
- 313
- References
- 40
- Citations by year
- 2026: 13 · 2025: 22 · 2024: 18 · 2023: 33 · 2022: 29 · 2021: 27 · 2020: 23 · 2019: 23 · 2018: 32 · 2017: 30 · 2016: 25 · 2015: 21 · 2014: 16
- FWCI
- 14.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Keywords
- signaling, endosomes, SLC15A4, dendritic cells, muramyl dipeptide, NOD-like receptors, NOD1, innate immunity, endosomal escape, TLR stimulation, inflammatory bowel disease, endocytosis, pattern recognition receptors
- MeSH terms
- Acetylmuramyl-Alanyl-Isoglutamine, Animals, Carrier Proteins, Cytoplasm, Dendritic Cells, Immunity, Innate, Inflammation, Ligands, Lysosomes, Nerve Tissue Proteins, Phagosomes, Endosomes, Salmonella typhimurium, Inflammatory Bowel Diseases, Membrane Transport Proteins, Mice, Nod2 Signaling Adaptor Protein
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