The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Regulated eukaryotic DNA replication origin firing with purified proteins
Open accessAbstract
Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated to promote precise genome duplication in every cell cycle. To accomplish this, initiation is partitioned into two temporally discrete steps: a double hexameric minichromosome maintenance (MCM) complex is first loaded at replication origins during G1 phase, and then converted to the active CMG (Cdc45–MCM–GINS) helicase during S phase. Here we describe the reconstitution of budding yeast DNA replication initiation with 16 purified replication factors, made from 42 polypeptides. Origin-dependent initiation recapitulates regulation seen in vivo. Cyclin-dependent kinase (CDK) inhibits MCM loading by phosphorylating the origin recognition complex (ORC) and promotes CMG formation by phosphorylating Sld2 and Sld3. Dbf4-dependent kinase (DDK) promotes replication by phosphorylating MCM, and can act either before or after CDK. These experiments define the minimum complement of proteins, protein kinase substrates and co-factors required for regulated eukaryotic DNA replication.
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DNA Replication in Eukaryotic Cells
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Concerted Loading of Mcm2–7 Double Hexamers around DNA during DNA Replication Origin Licensing
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Self-citationEvrin, C., Clarke, P., Zech, J., Lurz, R., Sun, J., Uhle, S., Li, H., Stillman, B., & Speck, C. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911500106 · Cited by 536
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Self-citationCyclin-dependent kinases prevent DNA re-replication through multiple mechanisms
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CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast
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Cited by
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells
van Nies, P., Westerlaken, I., Blanken, D., Salas, M., Mencía, M., & Danelon, C. (2018) · Nature Communications · doi:10.1038/s41467-018-03926-1 · Cited by 253
Mechanisms for initiating cellular DNA replication
Bleichert, F., Botchan, M. R., & Berger, J. M. (2017) · Science · doi:10.1126/science.aah6317 · Cited by 220
In silico protein interaction screening uncovers DONSON’s role in replication initiation
Lim, Y., Tamayo-Orrego, L., Schmid, E., Tarnauskaite, Z., Kochenova, O. V., Gruar, R., Muramatsu, S., Lynch, L., Schlie, A. V., Carroll, P. L., et al. (2023) · Science · doi:10.1126/science.adi3448 · Cited by 112
Nuclear PGK1 Alleviates ADP‐Dependent Inhibition of CDC7 to Promote DNA Replication
Li, X., Qian, X., Jiang, H., Xia, Y., Zheng, Y., Li, J., Huang, B.-J., Fang, J., Qian, C.-N., Jiang, T., et al. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.09.007 · Cited by 105
How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication
Jones, M. L., Aria, V., Baris, Y., & Yeeles, J. T. P. (2023) · Molecular Cell · doi:10.1016/j.molcel.2023.06.035 · Cited by 86
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Metadata
- Authors
- Yeeles, J. T. P., Deegan, T. D., Janska, A., Early, A., & Diffley, J. F. X.
- Venue
- Nature
- Published
- 2015-03-04
- Volume
- 519
- Issue
- 7544
- Pages
- 431-435
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2085365618
- Cited by
- 494
- References
- 34
- FWCI
- 23.29
- Type
- journal article
- Language
- en
- Open access
- green
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