The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells
Open accessAbstract
Replication of DNA-encoded information and its conversion into functional proteins are universal properties of life. In an effort toward the construction of a synthetic minimal cell, we implement here the DNA replication machinery of the Φ29 virus in a cell-free gene expression system. Amplification of a linear DNA template by self-encoded, de novo synthesized Φ29 proteins is demonstrated. Complete information transfer is confirmed as the copied DNA can serve as a functional template for gene expression, which can be seen as an autocatalytic DNA replication cycle. These results show how the central dogma of molecular biology can be reconstituted and form a cycle in vitro. Finally, coupled DNA replication and gene expression is compartmentalized inside phospholipid vesicles providing the chassis for evolving functions in a prospective synthetic cell relying on the extant biology.
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Citations
Cites
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Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K., & Ueda, T. (2001) · Nature Biotechnology · doi:10.1038/90802 · Cited by 1,940
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Design and synthesis of a minimal bacterial genome
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Highly Efficient DNA Synthesis by the Phage ϕ 29 DNA Polymerase
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Self-citationRegulated eukaryotic DNA replication origin firing with purified proteins
Yeeles, J. T. P., Deegan, T. D., Janska, A., Early, A., & Diffley, J. F. X. (2015) · Nature · doi:10.1038/nature14285 · Cited by 494
Towards synthesis of a minimal cell
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Cited by
Recent advances in the development of gene delivery systems.
Sung, Y., & Kim, S. (2019) · Biomaterials Research · doi:10.1186/s40824-019-0156-z · Cited by 458
Synthesis and applications of mirror-image proteins
Harrison, K., Mackay, A. S., Kambanis, L., Maxwell, J. W. C., & Payne, R. J. (2023) · Nature Reviews Chemistry · doi:10.1038/s41570-023-00493-y · Cited by 104
Reconstituting Natural Cell Elements in Synthetic Cells
Gaut, N. J., & Adamala, K. P. (2021) · Advanced Biology · doi:10.1002/adbi.202000188 · Cited by 92
Lopez, A., & Fiore, M. (2019) · Life · doi:10.3390/life9020049 · Cited by 89
Metadata
- Authors
- van Nies, P., Westerlaken, I., Blanken, D., Salas, M., Mencía, M., & Danelon, C.
- Venue
- Nature Communications
- Published
- 2018-04-20
- Volume
- 9
- Issue
- 1
- Pages
- 1583-1583
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2802610819
- Cited by
- 253
- References
- 39
- FWCI
- 14.34
- Type
- journal article
- Language
- en
- Open access
- gold
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- van Nies, P., Westerlaken, I., Blanken, D., Salas, M., Mencía, M., & Danelon, C. (2018). Self-replication of DNA by its encoded proteins in liposome-based synthetic cells. Nature Communications. https://doi.org/10.1038/s41467-018-03926-1
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
