The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Cloning and variation of ground state intestinal stem cells
Open accessAbstract
Stem cells of the gastrointestinal tract, pancreas, liver and other columnar epithelia collectively resist cloning in their elemental states. Here we demonstrate the cloning and propagation of highly clonogenic, ‘ground state’ stem cells of the human intestine and colon. We show that derived stem-cell pedigrees sustain limited copy number and sequence variation despite extensive serial passaging and display exquisitely precise, cell-autonomous commitment to epithelial differentiation consistent with their origins along the intestinal tract. This developmentally patterned and epigenetically maintained commitment of stem cells is likely to enforce the functional specificity of the adult intestinal tract. Using clonally derived colonic epithelia, we show that toxins A or B of the enteric pathogen Clostridium difficile recapitulate the salient features of pseudomembranous colitis. The stability of the epigenetic commitment programs of these stem cells, coupled with their unlimited replicative expansion and maintained clonogenicity, suggests certain advantages for their use in disease modelling and regenerative medicine.
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
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Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
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Self-citationFunctional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell
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Lgr5 stem cells are indispensable for radiation-induced intestinal regeneration
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Modeling Development and Disease with Organoids
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Organoids as an in vitro model of human development and disease
Fatehullah, A., Tan, S. H., & Barker, N. (2016) · Nature Cell Biology · doi:10.1038/ncb3312 · Cited by 1,508
Metagenome-wide association studies: fine-mining the microbiome
Wang, J., & Jia, H. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.83 · Cited by 429
Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
Mou, H., Vinarsky, V., Tata, P. R., Brazauskas, K., Choi, S. H., Crooke, A. K., Zhang, B., Solomon, G. M., Turner, B., Bihler, H., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.05.012 · Cited by 382
Saxena, K., Blutt, S. E., Ettayebi, K., Zeng, X.-L., Broughman, J. R., Crawford, S. E., Karandikar, U. C., Sastri, N. P., Conner, M. E., Opekun, A. R., et al. (2016) · Journal of Virology · doi:10.1128/jvi.01930-15 · Cited by 375
Metadata
- Authors
- Wang, X., Yamamoto, Y., Wilson, L. H., Zhang, T., Howitt, B. E., Farrow, M. A., Kern, F., Ning, G., Hong, Y., Khor, C. C., Chevalier, B., Bertrand, D., Wu, L., Nagarajan, N., Sylvester, F. A., Hyams, J. S., Devers, T., Bronson, R., Lacy, D. B., Ho, K. Y., Crum, C. P., McKeon, F., & Xian, W.
- Venue
- Nature
- Published
- 2015-06-03
- Volume
- 522
- Issue
- 7555
- Pages
- 173-178
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1910612799
- Cited by
- 188
- References
- 10
- FWCI
- 10.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, X., Yamamoto, Y., Wilson, L. H., Zhang, T., Howitt, B. E., Farrow, M. A., Kern, F., Ning, G., Hong, Y., Khor, C. C., Chevalier, B., Bertrand, D., Wu, L., Nagarajan, N., Sylvester, F. A., Hyams, J. S., Devers, T., Bronson, R., Lacy, D. B., . . . Xian, W. (2015). Cloning and variation of ground state intestinal stem cells. Nature. https://doi.org/10.1038/nature14484
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