The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
A robust system for production of minicircle DNA vectors
Open accessAbstract
Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Some researchers use, therefore, minicircle DNA vectors devoid of prokaryotic and antibiotic DNA sequences for their AAV production [228].”
From Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays (2020) · cites this paper as [228]
Experimental — accuracy not yet measuredThe check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“Downregulating the Y-box binding protein 1 (YB1) in HEK293T cells did increase the physical titer by 47× for rAAV2, yet it failed to improve the yield for rAAV5 sequences for their AAV production [228].”
From Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays (2020) · cites this paper as [228]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902
Cherepanov, P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,952
An efficient recombination system for chromosome engineering in Escherichia coli
Yu, D., Ellis, H. M., Lee, E.-C., Jenkins, N. A., Copeland, N. G., & Court, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.100127597 · Cited by 1,688
A nonviral minicircle vector for deriving human iPS cells
Jia, F., Wilson, K. D., Sun, N., Gupta, D. M., Huang, M., Li, Z., Panetta, N. J., Chen, Z. Y., Robbins, R. C., Kay, M. A., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1426 · Cited by 734
Self-citationChen, Z.-Y., He, C.-Y., Ehrhardt, A., & Kay, M. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00168-0 · Cited by 463
Self-citationThorpe, H. M., & Smith, M. C. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5505 · Cited by 434
Khlebnikov, A., Keasling, J. D., Wanner, B. L., Skaug, T., & Datsenko, K. A. (2001) · Microbiology · doi:10.1099/00221287-147-12-3241 · Cited by 289
A new DNA vehicle for nonviral gene delivery: supercoiled minicircle
Darquet, A.-M., Cameron, B., Wils, P., Scherman, D., & Crouzet, J. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300540 · Cited by 250
Tan, Y., Li, S., Pitt, B. R., & Huang, L. (1999) · Human Gene Therapy · doi:10.1089/10430349950017149 · Cited by 236
Chen, Z.-Y., He, C.-Y., & Kay, M. A. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.126 · Cited by 180
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–200 of 326
Lijkwan, M. A., Hellingman, A. A., Bos, E. J., van der Bogt, K. E. A., Huang, M., Kooreman, N. G., de Vries, M. R., Peters, H. A. B., Robbins, R. C., Hamming, J. F., et al. (2013) · Human Gene Therapy · doi:10.1089/hum.2013.110 · Cited by 34
Orav, M., Henno, L., Isok-Paas, H., Geimanen, J., Ustav, M., & Ustav, E. (2013) · Journal of Virology · doi:10.1128/jvi.01798-13 · Cited by 34
The Transcription Map of Human Papillomavirus Type 18 during Genome Replication in U2OS Cells
Toots, M., Männik, A., Kivi, G., Ustav, M., Ustav, E., & Ustav, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0116151 · Cited by 32
Li, F., Cheng, L., Murphy, C. M., Reszka-Blanco, N., Wu, Y., Chi, L., Hu, J., & Su, L. (2016) · Scientific Reports · doi:10.1038/srep36483 · Cited by 32
Retracted: Bacteria-free minicircle DNA system to generate integration-free CAR-T cells
Cheng, C., Tang, N., Li, J., Cao, S., Zhang, T.-T., Wei, X., & Wang, H. (2018) · Journal of Medical Genetics · doi:10.1136/jmedgenet-2018-105405 · Cited by 31
RetractedImproved intracellular delivery of peptide- and lipid-nanoplexes by natural glycosides
Weng, A., Manunta, M., Thakur, M., Gilabert‐Oriol, R., Tagalakis, A. D., Eddaoudi, A., Munye, M. M., Vink, C. A., Wiesner, B., Eichhorst, J., et al. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.03.007 · Cited by 30
Bishop, D. C., Caproni, L. J., Gowrishankar, K., Łęgiewicz, M., Karbowniczek, K., Tite, J. P., Gottlieb, D. J., & Micklethwaite, K. P. (2020) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2019.12.020 · Cited by 33
Pang, X., Ma, F., Zhang, P., Zhong, Y., Zhang, J., Wang, T., Zheng, G., Hou, X., Zhao, J., He, C., et al. (2016) · Human Gene Therapy · doi:10.1089/hum.2016.122 · Cited by 31
Self-citationMezzanotte, L., Blankevoort, V., Löwik, C. W. G. M., & Kaijzel, E. L. (2014) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-014-7917-2 · Cited by 29
Andrews, C. D., Luo, Y., Sun, M., Yu, J., Goff, A. J., Glass, P. J., Padte, N. N., Huang, Y., & Ho, D. D. (2017) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2017.09.003 · Cited by 29
Webber, B. R., Johnson, M. J., Skeate, J. G., Slipek, N. J., Lahr, W. S., DeFeo, A. P., Mills, L. J., Qiu, X., Rathmann, B., Diers, M. D., et al. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01157-4 · Cited by 29
Toots, M., Ustav, M., Männik, A., Mumm, K., Tämm, K., Tamm, T., Ustav, E., & Ustav, M. (2017) · PLoS Pathogens · doi:10.1371/journal.ppat.1006168 · Cited by 28
Zhao, J., Lu, D., Moya, S. E., Yan, H., Qiu, M., Chen, J., Wang, X., Li, Y., Pan, H., Chen, G., et al. (2020) · Applied Materials Today · doi:10.1016/j.apmt.2020.100701 · Cited by 28
Recent advances on HIV DNA vaccines development: Stepwise improvements to clinical trials
Rezaei, T., Khalili, S., Baradaran, B., Mosafer, J., Rezaei, S., Mokhtarzadeh, A., & de la Guárdia, M. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.10.045 · Cited by 28
Alam, T., Wai, P. Y., Held, D., Vakili, S. T. T., Forsberg, E., & Sollinger, H. W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067515 · Cited by 28
Lu, J., Williams, J. A., Luke, J., Zhang, F., Chu, K., & Kay, M. A. (2017) · Human Gene Therapy · doi:10.1089/hum.2016.140 · Cited by 26
Self-citationInitial amplification of the HPV18 genome proceeds via two distinct replication mechanisms
Orav, M., Geimanen, J., Sepp, E.-M., Henno, L., Ustav, E., & Ustav, M. (2015) · Scientific Reports · doi:10.1038/srep15952 · Cited by 26
Akita, H., Kurihara, D., Schmeer, M., Schleef, M., & Harashima, H. (2015) · Pharmaceutics · doi:10.3390/pharmaceutics7020064 · Cited by 26
Cardiac gene therapy: are we there yet?
Matkar, P. N., Leong-Poi, H. M., & Singh, K. K. (2016) · Gene Therapy · doi:10.1038/gt.2016.43 · Cited by 26
Minicircle Versus Plasmid DNA Delivery by Receptor-Targeted Polyplexes
Levačić, A. K., Morys, S., Kempter, S., Lächelt, U., & Wagner, E. (2017) · Human Gene Therapy · doi:10.1089/hum.2017.123 · Cited by 26
A New Strategy to Deliver Synthetic Protein Drugs: Self-reproducible Biologics Using Minicircles
Yi, H., Kim, Y., Kim, J., Jung, H., Rim, Y. A., Jung, S. M., Park, S., & Ju, J. H. (2014) · Scientific Reports · doi:10.1038/srep05961 · Cited by 25
Hou, X., Guo, X., Chen, Y., He, C., & Chen, Z. (2015) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2014.62 · Cited by 25
Self-citationIsok-Paas, H., Männik, A., Ustav, E., & Ustav, M. (2015) · Virology Journal · doi:10.1186/s12985-015-0292-6 · Cited by 25
Jiang, Y., Gao, X., Xu, K., Wang, J., Huang, H., Shi, C., Yang, W., Kang, Y., Curtiss, R., Yang, G., et al. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.00407-19 · Cited by 23
Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production
Wong, S. P., Argyros, O., & Harbottle, R. P. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-873-3_7 · Cited by 23
Gao, L., Chen, S., Hong, M., Zhou, W., Wang, B., Qiu, J., Xia, J., Zhao, P., Fu, L., Wang, J., et al. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00652-x · Cited by 22
Chen, G., Zeng, L., Bi, B., Huang, X., Qiu, M., Chen, P., Chen, Z., He, Y., Pan, Y., Chen, Y., et al. (2023) · Advanced Science · doi:10.1002/advs.202204654 · Cited by 22
Self-citationImpact of DNA Vector Topology on Non-Viral Gene Therapeutic Safety and Efficacy
Sum, C. S., Wettig, S. D., & Slavcev, R. A. (2014) · Current Gene Therapy · doi:10.2174/1566523214666140612154929 · Cited by 24
Minicircle Biopharmaceuticals–An Overview of Purification Strategies
Alves, C. P. A., Prazeres, D. M. F., & Monteiro, G. A. (2021) · Frontiers in Chemical Engineering · doi:10.3389/fceng.2020.612594 · Cited by 22
Multifunctional nanocarriers for codelivery of nucleic acids and chemotherapeutics to cancer cells
Gaspar, V. M., Moreira, A. F., de Melo‐Diogo, D., Costa, E. C., Queiroz, J. A., de Sousa, F. P., Pichon, C., & Correia, I. J. (2016) · Elsevier eBooks · doi:10.1016/b978-0-323-41736-5.00006-6 · Cited by 23
Nanogels: Smart tools to enlarge the therapeutic window of gene therapy
Espuche, B., Moya, S. E., & Calderón, M. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.123864 · Cited by 21
Detection of EPO gene doping in blood
Neuberger, E. W. I., Jurkiewicz, M., Moser, D., & Simon, P. D. (2012) · Drug Testing and Analysis · doi:10.1002/dta.1347 · Cited by 22
Development of a nicking endonuclease-assisted method for the purification of minicircles
Alves, C. P. A., Šimčíková, M., Brito, L., Monteiro, G. A., & Prazeres, D. M. F. (2016) · Journal of Chromatography A · doi:10.1016/j.chroma.2016.03.035 · Cited by 21
Exploring the role of NCCR variation on JC polyomavirus expression from dual reporter minicircles
L’Honneur, A.-S., Leh, H., Laurent-Tchénio, F., Hazan, U., Rozenberg, F., & Bury‐Moné, S. (2018) · PLoS ONE · doi:10.1371/journal.pone.0199171 · Cited by 21
Fernandes, A. R., & Chari, D. M. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.06.024 · Cited by 21
Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange
Kelton, W., Waindok, A. C., Pesch, T., Pogson, M., Ford, K., Parola, C., & Reddy, S. T. (2017) · Scientific Reports · doi:10.1038/srep45775 · Cited by 21
Versatile modification of the CRISPR/Cas9 ribonucleoprotein system to facilitate in vivo application
Sun, B., Chen, H., & Gao, X. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.08.007 · Cited by 20
Boura, J. S., Vance, M. A., Yin, W., Madeira, C. G., da Silva, C. L., Porada, C. D., & Almeida-Porada, G. D. (2014) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2014.41 · Cited by 21
Ronald, J., Cussó, L., Chuang, H.-Y., Yan, X., Dragulescu-Andrasi, A., & Gambhir, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073138 · Cited by 18
Delivery of DNA-Based Therapeutics for Treatment of Chronic Diseases
Sussman, C., Liberatore, R. A., & Drozdz, M. M. (2024) · Pharmaceutics · doi:10.3390/pharmaceutics16040535 · Cited by 19
Serra, J., Alves, C. P. A., Brito, L., Monteiro, G. A., Cabral, J. M. S., Prazeres, D. M. F., & da Silva, C. L. (2018) · Human Gene Therapy · doi:10.1089/hum.2018.154 · Cited by 19
A homology independent sequence replacement strategy in human cells using a CRISPR nuclease
Danner, E. W., Lebedin, M. Y., de la Rosa, K., & Kühn, R. (2021) · Open Biology · doi:10.1098/rsob.200283 · Cited by 22
Spinal cord injury induced neuropathic pain: Molecular targets and therapeutic approaches
Schomberg, D. T., Miranpuri, G. S., Duellman, T., Crowell, A. D., Vemuganti, R., & Resnick, D. K. (2015) · Metabolic Brain Disease · doi:10.1007/s11011-014-9642-0 · Cited by 20
Therapeutic Challenge of Minicircle Vector Encoding Klotho in Animal Model
Shin, Y., Luo, K., Quan, Y., Ko, E. J., Chung, B. H., Lim, S. W., & Yang, C. W. (2019) · American Journal of Nephrology · doi:10.1159/000499863 · Cited by 19
Bandara, N., Gurusinghe, S., Chen, H., Chen, S., Wang, L., Lim, S. Y., & Strappe, P. (2016) · Stem Cell Research & Therapy · doi:10.1186/s13287-016-0307-2 · Cited by 18
Chen, Y., Ouyang, M. J., Yan, R., Maarouf, M., Wang, X., Chen, B., Liu, S., Hu, J., Guo, G., Zhang, J., et al. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02641 · Cited by 17
Xu, X., Xing, Q., Liu, R., Liu, D., Yu, Z., Wang, Y., Zhou, P., Zhang, Y. V., Wang, J., Cao, Y., et al. (2022) · Stem Cells International · doi:10.1155/2022/5744538 · Cited by 17
Self in vivo production of a synthetic biological drug CTLA4Ig using a minicircle vector
Rim, Y. A., Yi, H., Kim, Y., Park, N., Jung, H., Kim, J., Jung, S. M., Park, S., & Ju, J. H. (2014) · Scientific Reports · doi:10.1038/srep06935 · Cited by 17
Stoller, F., Schlegel, A. A., Viecelli, H. M., Rüfenacht, V., Cesarovic, N., Viecelli, C., Deplazes, S., Bettschart‐Wolfensberger, R., Hurter, K., Schmierer, P. A., et al. (2015) · Human Gene Therapy Methods · doi:10.1089/hgtb.2014.140 · Cited by 17
Treatment of Cystathionine β-Synthase Deficiency in Mice Using a Minicircle-Based Naked DNA Vector
Lee, H., Gallego‐Villar, L., Grisch‐Chan, H. M., Häberle, J., Thöny, B., & Kruger, W. D. (2019) · Human Gene Therapy · doi:10.1089/hum.2019.014 · Cited by 17
Therapeutic Mechanism of Nucleic Acid Drugs
Liu, L., Gao, H., Guo, C., Liu, T., Li, N., & Qian, Q. (2021) · ChemistrySelect · doi:10.1002/slct.202002901 · Cited by 16
Kozisek, T., Hamann, A., Samuelson, L., Fudolig, M. A., & Pannier, A. K. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.06.018 · Cited by 16
Contemporary approaches for nonviral gene therapy.
Jones, C. H., Hill, A. B., Chen, M., & Pfeifer, B. A. (2015) · PubMed · Cited by 16
On the dual effect of glucose during production of pBAD/AraC-based minicircles
Šimčíková, M., Prather, K. L. J., Prazeres, D. M. F., & Monteiro, G. A. (2014) · Vaccine · doi:10.1016/j.vaccine.2014.02.035 · Cited by 16
Advances in Overcoming Immune Responses following Hemophilia Gene Therapy
H. Miao, C. (2012) · Journal of Genetic Syndromes & Gene Therapy · doi:10.4172/2157-7412.s1-007 · Cited by 15
Minicircle-oriP-miR-31 as a Novel EBNA1-Specific miRNA Therapy Approach for Nasopharyngeal Carcinoma
Wu, J., Tan, X., Lin, J., Yuan, L., Chen, J., Qiu, L., & Huang, W. (2017) · Human Gene Therapy · doi:10.1089/hum.2016.136 · Cited by 15
Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching
Nakamura, S., Ishihara, M., Watanabe, S., Ando, N., Ohtsuka, M., & Sato, M. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19113452 · Cited by 15
Šimčíková, M., Alves, C. P. A., Brito, L., Prather, K. L. J., Prazeres, D. M. F., & Monteiro, G. A. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7565-x · Cited by 15
Minicircle DNA purification using a CIM® DEAE‐1 monolithic support
Diamantino, T., Pereira, P., Queiroz, J. A., Sousa, Â., & de Sousa, F. P. (2016) · Journal of Separation Science · doi:10.1002/jssc.201600375 · Cited by 15
Wang, X., Alshehri, F., Manzanares, D., Li, Y., He, Z., Qiu, B., Zeng, M., Sigen, A., Lara‐Sáez, I., & Wang, W. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222312774 · Cited by 15
Ding, J., Yi, Z., Zai, W., Wu, M., Chen, B., Cai, Q., Zhang, X., & Yuan, Z. (2023) · mBio · doi:10.1128/mbio.03550-22 · Cited by 14
Hou, X., Jiao, R., Guo, X., Wang, T., Chen, P., Wang, D., Chen, Y., He, C.-Y., & Chen, Z.-Y. (2016) · Gene Therapy · doi:10.1038/gt.2016.37 · Cited by 14
Self-citationErythrocyte membrane based cationic polymer-mcDNA complexes as an efficient gene delivery system
Huang, P., Zhao, J., Wei, C., Hou, X., Chen, P., Tan, Y., He, C., Wang, Z., & Chen, Z. (2016) · Biomaterials Science · doi:10.1039/c6bm00638h · Cited by 14
Self-citationMinicircle Delivery to the Neural Retina as a Gene Therapy Approach
Staurenghi, F., McClements, M. E., Salman, A., & MacLaren, R. E. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911673 · Cited by 14
Lin, D., Lei, L., Zhang, Y., Hu, B., Bao, G., Liu, Y., Song, Y., Liu, C., Wu, Y., Zhao, L., et al. (2015) · European Journal of Immunology · doi:10.1002/eji.201445195 · Cited by 14
Multimodal chromatography of supercoiled minicircles: A closer look into DNA-ligand interactions
Silva-Santos, A. R., Alves, C. P. A., Monteiro, G. A., Azevedo, A. M., & Prazeres, D. M. F. (2018) · Separation and Purification Technology · doi:10.1016/j.seppur.2018.11.015 · Cited by 14
Li, L., Dimitriadis, E. K., Yang, Y., Li, J., Yuan, Z., Qiao, C., Beley, C., Smith, R. H., García, L., & Kotin, R. M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069879 · Cited by 15
Maximization of the Minicircle DNA Vaccine Production Expressing SARS-CoV-2 RBD
Ventura, C., Eusébio, D., Gonçalves, A. M., Barroca-Ferreira, J., Costa, D. R. B., Cui, Z., Passarinha, L. A., & Sousa, Â. (2022) · Biomedicines · doi:10.3390/biomedicines10050990 · Cited by 13
Geguchadze, R., Wang, Z., Zourelias, L., Riveros, P. P., Edwards, P. C., Machen, L. L., & Passineau, M. J. (2014) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2014.7 · Cited by 13
Chan, T., Grisch‐Chan, H. M., Schmierer, P. A., Subotic, U., Rimann, N., Scherer, T., Hetzel, U., Bozza, M., Harbottle, R. P., Williams, J. A., et al. (2022) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2022.01.006 · Cited by 13
Hahn, P. A., & Martins, M. A. (2023) · Journal of Virus Eradication · doi:10.1016/j.jve.2023.100316 · Cited by 15
Safety Assessment of Biolistic DNA Vaccination
Langer, B., Renner, M., Scherer, J., Schüle, S., & Cichutek, K. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-110-3_27 · Cited by 14
DNA Vaccines for the Induction of Immune Responses in Mucosal Tissues
Raška, M., & Tuřánek, J. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-415847-4.00067-7 · Cited by 13
Improving therapeutic potential of non-viral minimized DNA vectors
Arevalo-Soliz, L. M., Hardee, C. L., Fogg, J. M., Corman, N. R., Noorbakhsh, C., & Zechiedrich, L. (2020) · Cell and Gene Therapy Insights · doi:10.18609/cgti.2020.163 · Cited by 14
Gene Delivery Techniques for Adult Stem Cell-Based Regenerative Therapy
Seo, S.-J., Kim, T.-H., Choi, S., Park, J.-H., Wall, I. B., & Kim, H. (2013) · Nanomedicine · doi:10.2217/nnm.13.165 · Cited by 12
Sensitive Detection of Peptide–Minicircle DNA Interactions by Surface Plasmon Resonance
Gaspar, V. M., Cruz, C., Queiroz, J. A., Pichon, C., Correia, I. J., & de Sousa, F. P. (2013) · Analytical Chemistry · doi:10.1021/ac303288x · Cited by 12
Replication Kinetics for a Reporter Merkel Cell Polyomavirus
Abere, B., Zhou, H., Shuda, M., Stolz, D. B., Rapchak, K., Moore, P. S., & Chang, Y. (2022) · Viruses · doi:10.3390/v14030473 · Cited by 12
Genetically engineered stem cell‐based strategies for articular cartilage regeneration
Santhagunam, A., Madeira, C. G., & Cabral, J. M. S. (2012) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1016 · Cited by 12
Alves, C. P. A., Šimčíková, M., Brito, L., Monteiro, G. A., & Prazeres, D. M. F. (2018) · Human Gene Therapy Methods · doi:10.1089/hgtb.2018.046 · Cited by 12
Han, B., Zhang, Y., Bi, X., Zhou, Y., Krueger, C. J., Hu, X., Zhu, Z., Tong, X., & Zhang, B. (2020) · Protein & Cell · doi:10.1007/s13238-020-00747-1 · Cited by 12
Review: Development of SARS-CoV-2 immuno-enhanced COVID-19 vaccines with nano-platform
Sa-nguanmoo, N., Namdee, K., Khongkow, M., Ruktanonchai, U., Zhao, Y., & Liang, X. (2021) · Nano Research · doi:10.1007/s12274-021-3832-y · Cited by 12
A survivin-driven, tumor-activatable minicircle system for prostate cancer theranostics
Wang, T., Chen, Y., Goodale, D. B., Allan, A. L., & Ronald, J. A. (2021) · Molecular Therapy — Oncolytics · doi:10.1016/j.omto.2021.01.007 · Cited by 12
Microbiome engineering: unlocking therapeutic potential in inflammatory bowel disease
Chen, K., Huang, L.-B., Yang, M., Yue, N.-N., Luo, D., Zhang, Y., Tian, C., Song, Y., Wei, D., Shi, R., et al. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1610029 · Cited by 11
Treating genetic blood disorders in the era of CRISPR-mediated genome editing
Almotiri, A., Abogosh, A., Abdelfattah, A., Alowaisy, D., & Rodrigues, N. P. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.01.031 · Cited by 11
Engineering a photoactivatable A-to-I RNA base editor for gene therapy in vivo
Li, H., Qiu, Y., Song, B., Quan, X., Zhang, D., Li, X., Yang, J., Liu, X., Zeng, Z., Ji, J., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02610-2 · Cited by 11
The ergodicity question when imaging DNA conformation using liquid cell electron microscopy
Li, J., Liu, F., Xu, J., Kim, Y., Kwon, O., Xia, B., Wang, H., & Granick, S. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2314797121 · Cited by 11
Orav, M., Gagnon, D., & Archambault, J. (2019) · mBio · doi:10.1128/mbio.00152-19 · Cited by 11
Kacherovsky, N. A., Liu, G. W., Jensen, M. C., & Pun, S. H. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25538 · Cited by 11
Chanani, P., Rezaei, N., Dormiani, K., Shokatian, M., & Ata-abadi, N. S. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102682 · Cited by 13
Jones, C. H., Håkansson, A., & Pfeifer, B. A. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb01058b · Cited by 10
Success of nano-vaccines against COVID-19: a transformation in nanomedicine
Sarangi, M. K., Padhi, S. K., Rath, G., Nanda, S. S., & Yi, D. K. (2022) · Expert Review of Vaccines · doi:10.1080/14760584.2022.2148659 · Cited by 11
Wang, X., Cai, C., Lv, W., Chen, K., Li, J., Liao, K., Zhang, Y., Huang, H., Lin, Y., Rong, Z., et al. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02554-w · Cited by 10
An in vitro approach for production of non-scar minicircle DNA vectors
Dong, Y., Aied, A., Li, J., Wang, Q., Hu, X., & Wang, W. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.04.008 · Cited by 10
Henno, L., Tombak, E.-M., Geimanen, J., Orav, M., Ustav, E., & Ustav, M. (2017) · Current Protocols in Microbiology · doi:10.1002/cpmc.28 · Cited by 10
Design, Construction, and Application of Transcription Activation-Like Effectors
Deng, P., Carter, S., & Fink, K. D. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9065-8_3 · Cited by 10
High-efficiency nonhomologous insertion of a foreign gene into the herpes simplex virus genome
Gong, Y., Bi, Y., Li, Z., Li, Y., Yao, Y., Long, Q., Pu, T., Chen, C., Liu, T., Dong, S., et al. (2020) · Journal of General Virology · doi:10.1099/jgv.0.001451 · Cited by 9
A versatile plasmid architecture for mammalian synthetic biology (VAMSyB)
Haellman, V., Strittmatter, T., Bertschi, A., Stücheli, P., & Fussenegger, M. (2021) · Metabolic Engineering · doi:10.1016/j.ymben.2021.04.003 · Cited by 9
Antibiotic-Free Gene Vectors: A 25-Year Journey to Clinical Trials
Marie, C., & Scherman, D. (2024) · Genes · doi:10.3390/genes15030261 · Cited by 9
Wu, X. T., Liu, G., Mu, M., Peng, Y., Li, X., Deng, L., Zhang, Z., Chen, M., You, S., & Kong, X. (2016) · Human Gene Therapy · doi:10.1089/hum.2016.006 · Cited by 9
A non-viral genome editing platform for site-specific insertion of large transgenes
Chaudhari, N., Rickard, A. M., Roy, S., Dröge, P., & Makhija, H. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-01890-6 · Cited by 10
Metadata
- Authors
- Kay, M. A., He, C.-Y., & Chen, Z.-Y.
- Affiliations
- Stanford Medicine (US)
- Corresponding author
- Kay, Mark A
- ORCID
- Kay, Mark A
- Venue
- Nature Biotechnology
- Published
- 2010-11-21
- Volume
- 28
- Issue
- 12
- Pages
- 1287-1289
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC4144359
- OpenAlex
- W2079903460
- Cited by
- 329
- References
- 18
- Citations by year
- 2026: 12 · 2025: 20 · 2024: 21 · 2023: 20 · 2022: 17 · 2021: 28 · 2020: 20 · 2019: 22 · 2018: 19 · 2017: 17 · 2016: 28 · 2015: 24 · 2014: 31 · 2013: 23 · 2012: 16
- FWCI
- 6.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL064274, R01HL064274)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- transgene expression, gene transfer
- MeSH terms
- Biotechnology, Cloning, Molecular, DNA, Circular, Escherichia coli, Genetic Vectors, Plasmids
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review