Cited by 329RNA Interference and Gene DeliveryBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

A robust system for production of minicircle DNA vectors

Open access

Kay, Mark A, He, Cheng-Yi, Chen, Zhi-Ying·2010·Nature Biotechnology

Open this paper in CoChat — free account

Abstract

Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nbt.1708

Cites

18 of 18 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
Cherepanov, P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,952
An efficient recombination system for chromosome engineering in Escherichia coli
Yu, D., Ellis, H. M., Lee, E.-C., Jenkins, N. A., Copeland, N. G., & Court, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.100127597 · Cited by 1,688
A nonviral minicircle vector for deriving human iPS cells
Jia, F., Wilson, K. D., Sun, N., Gupta, D. M., Huang, M., Li, Z., Panetta, N. J., Chen, Z. Y., Robbins, R. C., Kay, M. A., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1426 · Cited by 734
Self-citation
Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivo
Chen, Z.-Y., He, C.-Y., Ehrhardt, A., & Kay, M. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00168-0 · Cited by 463
Self-citation
In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family
Thorpe, H. M., & Smith, M. C. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5505 · Cited by 434
Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter
Khlebnikov, A., Keasling, J. D., Wanner, B. L., Skaug, T., & Datsenko, K. A. (2001) · Microbiology · doi:10.1099/00221287-147-12-3241 · Cited by 289
A new DNA vehicle for nonviral gene delivery: supercoiled minicircle
Darquet, A.-M., Cameron, B., Wils, P., Scherman, D., & Crouzet, J. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300540 · Cited by 250
The Inhibitory Role of CpG Immunostimulatory Motifs in Cationic Lipid Vector-Mediated Transgene Expression in Vivo
Tan, Y., Li, S., Pitt, B. R., & Huang, L. (1999) · Human Gene Therapy · doi:10.1089/10430349950017149 · Cited by 236
Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo
Chen, Z.-Y., He, C.-Y., & Kay, M. A. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.126 · Cited by 180
Self-citation

Flagged first, then by citations

Show all 18 references

Sorted by citations

Non-viral vectors for gene-based therapy
Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects
Li, H., Yang, Y., Hong, W., Huang, M., Wu, M., & Zhao, X. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0089-y · Cited by 1,829
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration
Suzuki, K., Tsunekawa, Y., Hernandez-Benitez, R., Wu, J., Zhu, J., Kim, E. J., Hatanaka, F., Yamamoto, M., Araoka, T., Li, Z., et al. (2016) · Nature · doi:10.1038/nature20565 · Cited by 1,150
State-of-the-art gene-based therapies: the road ahead
Kay, M. A. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2971 · Cited by 699
Self-citation
A Multifunctional AAV-CRISPR-Cas9 and Its Host Response
Chew, W. L., Tabebordbar, M., Cheng, J. K. W., Mali, P., Wu, E. Y., Ng, A. H. M., Zhu, K., Wagers, A. J., & Church, G. M. (2016) · Nature Methods · doi:10.1038/nmeth.3993 · Cited by 617
Engineering CAR-T cells
Zhang, C., Liu, J., Zhong, J. F., & Zhang, X. (2017) · Biomarker Research · doi:10.1186/s40364-017-0102-y · Cited by 578
Niche stiffness underlies the ageing of central nervous system progenitor cells
Segel, M., Neumann, B., Hill, M. F. E., Weber, I. P., Viscomi, C., Zhao, C., Young, A., Agley, C. C., Thompson, A. J., Gonzalez, G. A., et al. (2019) · Nature · doi:10.1038/s41586-019-1484-9 · Cited by 532
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
Nakade, S., Tsubota, T., Sakane, Y., Kume, S., Sakamoto, N., Obara, M., Daimon, T., Sezutsu, H., Yamamoto, T., Sakuma, T., et al. (2014) · Nature Communications · doi:10.1038/ncomms6560 · Cited by 503
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L., Zhou, W., Jiang, K., Garushyants, S. K., Roberts, N., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01527-4 · Cited by 520
Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
Jones, C. H., Chen, C., Ravikrishnan, A., Rane, S., & Pfeifer, B. A. (2013) · Molecular Pharmaceutics · doi:10.1021/mp400467x · Cited by 430
Molecular mechanisms for enhanced DNA vaccine immunogenicity
Li, L., & Petrovsky, N. (2015) · Expert Review of Vaccines · doi:10.1586/14760584.2016.1124762 · Cited by 336
Hepatitis B Virus X Protein Promotes Degradation of SMC5/6 to Enhance HBV Replication
Murphy, C. M., Xu, Y., Li, F., Nio, K., Reszka-Blanco, N., Li, X., Wu, Y., Yu, Y., Xiong, Y., & Su, L. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.08.026 · Cited by 312
Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joiningPreprint
Maresca, M., Lin, V. G., Guo, N., & Yang, Y. (2012) · Genome Research · doi:10.1101/gr.145441.112 · Cited by 310
Targeted Delivery of CRISPR/Cas9‐Mediated Cancer Gene Therapy via Liposome‐Templated Hydrogel Nanoparticles
Chen, Z., Liu, F., Chen, Y., Liu, J., Wang, X., Chen, A. T., Deng, G., Zhang, H., Liu, J., Hong, Z., et al. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201703036 · Cited by 297
Self-citation
Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors
Monjezi, R., Miskey, C., Gogishvili, T., Schleef, M., Schmeer, M., Einsele, H., Ivics, Z., & Hudecek, M. (2016) · Leukemia · doi:10.1038/leu.2016.180 · Cited by 268
Viscous control of cellular respiration by membrane lipid composition
Budin, I., de Rond, T., Chen, Y., Chan, L. J. G., Petzold, C. J., & Keasling, J. D. (2018) · Science · doi:10.1126/science.aat7925 · Cited by 223
Resolving systematic errors in widely used enhancer activity assays in human cells
Muerdter, F., Boryń, Ł. M., Woodfin, A. R., Neumayr, C., Rath, M., Zabidi, M. A., Pagani, M., Haberle, V., Kazmar, T., Catarino, R. R., et al. (2018) · Nature Methods · doi:10.1038/nmeth.4534 · Cited by 208
Challenges and strategies for next-generation bispecific antibody-based antitumor therapeutics
Li, H., Saw, P. E., & Song, E. (2020) · Cellular and Molecular Immunology · doi:10.1038/s41423-020-0417-8 · Cited by 205
CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
Schmid-Burgk, J. L., Höning, K., Ebert, T. S., & Hornung, V. (2016) · Nature Communications · doi:10.1038/ncomms12338 · Cited by 197
The future of human DNA vaccines
Li, L., Saadé, F., & Petrovsky, N. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.08.012 · Cited by 183
Engineering DNA vaccines against infectious diseases
Lee, J., Kumar, S. A., Jhan, Y., & Bishop, C. J. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.08.033 · Cited by 177
Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
Williams, J. (2013) · Vaccines · doi:10.3390/vaccines1030225 · Cited by 166
A Guide to Nucleic Acid Vaccines in the Prevention and Treatment of Infectious Diseases and Cancers: From Basic Principles to Current Applications
Qin, F., Xia, F., Chen, H., Cui, B., Feng, Y., Zhang, P., Chen, J., & Luo, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.633776 · Cited by 167
Engagement of the ATR-Dependent DNA Damage Response at the Human Papillomavirus 18 Replication Centers during the Initial Amplification
Reinson, T., Toots, M., Kadaja, M., Pipitch, R., Allik, M., Ustav, E., & Ustav, M. (2012) · Journal of Virology · doi:10.1128/jvi.01943-12 · Cited by 141
Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx–DDB1 Interaction
Sekiba, K., Otsuka, M., Ohno, M., Yamagami, M., Kishikawa, T., Suzuki, T., Ishibashi, R., Seimiya, T., Tanaka, E., & Koike, K. (2018) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2018.10.010 · Cited by 126
Minicircle DNA Vectors Achieve Sustained Expression Reflected by Active Chromatin and Transcriptional Level
Gracey, L. E., Maniar, J. M., Chen, Z.-Y., Lu, J., Fire, A. Z., & Kay, M. A. (2012) · Molecular Therapy · doi:10.1038/mt.2012.244 · Cited by 123
Self-citation
Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors
Yu, Y., Wu, X., Guan, N., Shao, J., Li, H., Chen, Y., Yuan, P., Li, D., & Ye, H. (2020) · Science Advances · doi:10.1126/sciadv.abb1777 · Cited by 117
New Generation of Plasmid Backbones Devoid of Antibiotic Resistance Marker for Gene Therapy Trials
Vandermeulen, G., Marie, C., Scherman, D., & Véronique Préat, A. (2011) · Molecular Therapy · doi:10.1038/mt.2011.182 · Cited by 113
Systematic evasion of the restriction-modification barrier in bacteria
Johnston, C. D., Cotton, S. L., Rittling, S. R., Starr, J. R., Borisy, G. G., Dewhirst, F. E., & Lemon, K. P. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1820256116 · Cited by 111
Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation
Wei, L., & Ploß, A. (2020) · Nature Microbiology · doi:10.1038/s41564-020-0678-0 · Cited by 110
Gene therapy for monogenic liver diseases: clinical successes, current challenges and future prospects
Baruteau, J., Waddington, S. N., Alexander, I. E., & Gissen, P. (2017) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-017-0053-3 · Cited by 111
Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
Wei, L., & Ploß, A. (2021) · Nature Communications · doi:10.1038/s41467-021-21850-9 · Cited by 105
Activation of melatonin receptor 2 but not melatonin receptor 1 mediates melatonin‐conferred cardioprotection against myocardial ischemia/reperfusion injury
Han, D. H., Wang, Y., Chen, J., Zhang, J., Yu, P., Zhang, R., Li, S., Bo, T., Wang, Y., Qiu, Y. F., et al. (2019) · Journal of Pineal Research · doi:10.1111/jpi.12571 · Cited by 93
IL-17A gene transfer induces bone loss and epidermal hyperplasia associated with psoriatic arthritis
Adamopoulos, I. E., Suzuki, É., Chao, C., Gorman, D., Adda, S., Maverakis, E., Zarbalis, K. S., Geissler, R. H., Asio, A., Blumenschein, W. M., et al. (2014) · Annals of the Rheumatic Diseases · doi:10.1136/annrheumdis-2013-204782 · Cited by 93
Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays
Buck, T., & Wijnholds, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124197 · Cited by 92
“Some researchers use, therefore, minicircle DNA vectors devoid of prokaryotic and antibiotic DNA sequences for their AAV production [228].”
Poly(2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics
Gaspar, V. M., Gonçalves, C., de Melo-Diogo, D., Costa, E. C., Queiroz, J. A., Pichon, C., Sousa, F., & Correia, I. J. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.06.040 · Cited by 91
Biomaterials as vectors for the delivery of CRISPR–Cas9
Eoh, J. H., & Gu, L. (2019) · Biomaterials Science · doi:10.1039/c8bm01310a · Cited by 92
Minicircle DNA vectors for gene therapy: advances and applications
Gaspar, V. M., de Melo‐Diogo, D., Costa, E. C., Moreira, A. F., Queiroz, J. A., Pichon, C., Correia, I. J., & de Sousa, F. P. (2014) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2015.996544 · Cited by 97
Expanding the scope of site‐specific recombinases for genetic and metabolic engineering
Gaj, T., Sirk, S. J., & Barbas, C. F. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.25096 · Cited by 91
Antibiotic-free selection in biotherapeutics: Now and forever
Mignon, C., Sodoyer, R., & Werle, B. (2015) · Pathogens · doi:10.3390/pathogens4020157 · Cited by 87
A comprehensive comparison of DNA and RNA vaccines
Wang, C., & Yuan, F. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115340 · Cited by 90
State-of-the-art human gene therapy: part I. Gene delivery technologies.
Wang, D., & Gao, G. (2014) · PubMed · Cited by 87
Versatile Trans-Replication Systems for Chikungunya Virus Allow Functional Analysis and Tagging of Every Replicase Protein
Utt, A., Quirin, T., Saul, S., Hellström, K., Ahola, T., & Merits, A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0151616 · Cited by 83
Nanotechnology and immunoengineering: How nanotechnology can boost CAR-T therapy
Nawaz, W., Xu, S., Li, Y., Huang, B., Wu, X., & Wu, Z. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.04.015 · Cited by 82
HBVcircle: A novel tool to investigate hepatitis B virus covalently closed circular DNA
Yan, Z., Zeng, J., Yu, Y., Xiang, K., Hu, H., Zhou, X., Gu, L., Wang, L., Zhao, J., Young, J. A. T., et al. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.02.004 · Cited by 80
Nanomedicine-based combination anticancer therapy between nucleic acids and small-molecular drugs
Huang, W., Chen, L., Kang, L., Jin, M., Sun, P., Xin, X., Gao, Z., & Bae, Y. H. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.06.004 · Cited by 77
Immune Cell Hacking: Challenges and Clinical Approaches to Create Smarter Generations of Chimeric Antigen Receptor T Cells
Elahi, R., Khosh, E., Tahmasebi, S., & Esmaeilzadeh, A. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01717 · Cited by 82
Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy
Tipanee, J., Samara-Kuko, E., Gevaert, T., Chuah, M. K., & VandenDriessche, T. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.06.006 · Cited by 76
Safety and efficacy of DNA vaccines
Stenler, S., Blomberg, P., & Smith, C. E. (2014) · Human Vaccines & Immunotherapeutics · doi:10.4161/hv.28077 · Cited by 74
Treatment of phenylketonuria using minicircle-based naked-DNA gene transfer to murine liver
Viecelli, H. M., Harbottle, R. P., Wong, S. P., Schlegel, A. A., Chuah, M., VandenDriessche, T., Harding, C. O., & Thöny, B. (2014) · Hepatology · doi:10.1002/hep.27104 · Cited by 76
Minicircle DNA Provides Enhanced and Prolonged Transgene Expression Following Airway Gene Transfer
Munye, M. M., Tagalakis, A. D., Barnes, J. L., Brown, R. E., McAnulty, R. J., Howe, S. J., & Hart, S. L. (2016) · Scientific Reports · doi:10.1038/srep23125 · Cited by 72
Converting extracellular vesicles into nanomedicine: loading and unloading of cargo
Joshi, B. S., Ortíz-Rios, D., & Zuhorn, I. S. (2021) · Materials Today Nano · doi:10.1016/j.mtnano.2021.100148 · Cited by 71
Microvesicle-Mediated Delivery of Minicircle DNA Results in Effective Gene-Directed Enzyme Prodrug Cancer Therapy
Kanada, M., Kim, B. D., Hardy, J. W., Ronald, J. A., Bachmann, M. H., Bernard, M. P., Perez, G. I., Zarea, A. A., Ge, T. J., Withrow, A., et al. (2019) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-19-0299 · Cited by 69
Derivation of Human Induced Pluripotent Stem Cells for Cardiovascular Disease Modeling
Narsinh, K., Narsinh, K. H., & Wu, J. C. (2011) · Circulation Research · doi:10.1161/circresaha.111.240374 · Cited by 70
Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery
Wan, Q., Xie, L., Gao, L., Wang, Z., Nan, X., Lei, H., Long, X., Chen, Z.-Y., He, C.-Y., Liu, G., et al. (2012) · Nanoscale · doi:10.1039/c2nr32438e · Cited by 64
Self-citation
The Extragenic Spacer Length Between the 5′ and 3′ Ends of the Transgene Expression Cassette Affects Transgene Silencing From Plasmid-based Vectors
Lu, J., Zhang, F., Xu, S., Fire, A. Z., & Kay, M. A. (2012) · Molecular Therapy · doi:10.1038/mt.2012.65 · Cited by 64
Self-citation
DNA vaccines for prostate cancer
Zahm, C. D., Colluru, V. T., & McNeel, D. G. (2017) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.02.016 · Cited by 63
Novel codon-optimized mini-intronic plasmid for efficient, inexpensive and xeno-free induction of pluripotency
Diecke, S., Lu, J., Lee, J., Termglinchan, V., Kooreman, N. G., Burridge, P. W., Ebert, A., Churko, J. M., Sharma, A., Kay, M. A., et al. (2015) · Scientific Reports · doi:10.1038/srep08081 · Cited by 62
Self-citation
Improving DNA Vaccine Performance Through Vector Design
Williams, J. (2014) · Current Gene Therapy · doi:10.2174/156652321403140819122538 · Cited by 63
Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8+ T-cell Responses
Dietz, W., Skinner, N., Hamilton, S. E., Jund, M. D., Heitfeld, S. M., Litterman, A. J., Hwu, P., Chen, Z.-Y., Salazar, A. Μ., Ohlfest, J. R., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2013.85 · Cited by 62
Self-citation
Nonintegrating Knockdown and Customized Scaffold Design Enhances Human Adipose-Derived Stem Cells in Skeletal Repair
Lévi, B., Hyun, J. S., Nelson, E. R., Li, S., Montoro, D. T., Wan, D. C., Jia, F., Glotzbach, J. P., James, A. W., Lee, M., et al. (2011) · Stem Cells · doi:10.1002/stem.757 · Cited by 61
How Far Are Non-Viral Vectors to Come of Age and Reach Clinical Translation in Gene Therapy?
Sainz-Ramos, M., Gallego, I., Villate-Beitia, I., Sesma, J. Z., Maldonado, I., Puras, G., & Pedraz, J. L. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147545 · Cited by 67
Comparative analysis of enzymatically produced novel linear DNA constructs with plasmids for use as DNA vaccines
Walters, A. A., Kinnear, E., Shattock, R. J., McDonald, J. U., Caproni, L. J., Porter, N., & Tregoning, J. S. (2014) · Gene Therapy · doi:10.1038/gt.2014.37 · Cited by 58
Vectorology and Factor Delivery in Induced Pluripotent Stem Cell Reprogramming
Hu, K. (2014) · Stem Cells and Development · doi:10.1089/scd.2013.0621 · Cited by 61
Pevonedistat, a Neuronal Precursor Cell‐Expressed Developmentally Down‐Regulated Protein 8–Activating Enzyme Inhibitor, Is a Potent Inhibitor of Hepatitis B Virus
Sekiba, K., Otsuka, M., Ohno, M., Yamagami, M., Kishikawa, T., Seimiya, T., Suzuki, T., Tanaka, E., Ishibashi, R., Funato, K., et al. (2018) · Hepatology · doi:10.1002/hep.30491 · Cited by 57
A Mini-intronic Plasmid (MIP): A Novel Robust Transgene Expression Vector In Vivo and In Vitro
Lu, J., Zhang, F., & Kay, M. A. (2013) · Molecular Therapy · doi:10.1038/mt.2013.33 · Cited by 54
Self-citation
Mapping of Betapapillomavirus Human Papillomavirus 5 Transcription and Characterization of Viral-Genome Replication Function
Sankovski, E., Männik, A., Geimanen, J., Ustav, E., & Ustav, M. (2013) · Journal of Virology · doi:10.1128/jvi.01841-13 · Cited by 53
Detecting cancers through tumor-activatable minicircles that lead to a detectable blood biomarker
Ronald, J. A., Chuang, H., Dragulescu‐Andrasi, A., Hori, S. S., & Gambhir, S. S. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1414156112 · Cited by 52
Mapping the Interactions of HBV cccDNA with Host Factors
Mohd-Ismail, N. K., Lim, Z., Gunaratne, J., & Tan, Y. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20174276 · Cited by 51
Non-viral therapeutic approaches to ocular diseases: An overview and future directions
Zulliger, R., Conley, S. M., & Naash, M. I. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.10.007 · Cited by 50
Managing MicroRNAs with Vector-Encoded Decoy-Type Inhibitors
Bak, R. O., Hollensen, A. K., & Mikkelsen, J. G. (2013) · Molecular Therapy · doi:10.1038/mt.2013.113 · Cited by 49
Vaccine development for bacterial pathogens: Advances, challenges and prospects
Mba, I. E., Sharndama, H. C., Anyaegbunam, Z. K. G., Anekpo, C. C., Amadi, B. C., Daji, M., Doowuese, Y., Ihezuo, U. J., Chukwukelu, J. U., & Okeke, O. P. (2023) · Tropical Medicine & International Health · doi:10.1111/tmi.13865 · Cited by 47
Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing
Gonçalves, G. A. L., Bower, D. M., Prazeres, D. M. F., Monteiro, G. A., & Prather, K. L. J. (2011) · Biotechnology Journal · doi:10.1002/biot.201100062 · Cited by 48
The Cell Cycle Timing of Human Papillomavirus DNA Replication
Reinson, T., Henno, L., Toots, M., Ustav, M., & Ustav, M. (2015) · PLoS ONE · doi:10.1371/journal.pone.0131675 · Cited by 47
Merkel Cell Polyomavirus Encodes Circular RNAs (circRNAs) Enabling a Dynamic circRNA/microRNA/mRNA Regulatory Network
Abere, B., Zhou, H., Li, J., Cao, S., Toptan, T., Grundhoff, A. T., Fischer, N., Moore, P. S., & Chang, Y. (2020) · mBio · doi:10.1128/mbio.03059-20 · Cited by 47
In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses
Wang, Q., Jiang, W., Chen, Y., Liu, P., Sheng, C., Chen, S., Zhang, H., Pan, C., Gao, S., & Huang, W. (2013) · Journal of Virology · doi:10.1128/jvi.02757-13 · Cited by 46
Cell Culture Models and Animal Models for HBV Study
Li, F., Wang, Z., Hu, F., & Su, L. (2019) · Advances in experimental medicine and biology · doi:10.1007/978-981-13-9151-4_5 · Cited by 45
The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely
Guo, X., Chen, P., Hou, X., Xu, W., Wang, D., Wang, T.-Y., Zhang, L., Zheng, G., Gao, Z., He, C., et al. (2016) · Scientific Reports · doi:10.1038/srep25552 · Cited by 44
Self-citation
A Chikungunya Virus trans -Replicase System Reveals the Importance of Delayed Nonstructural Polyprotein Processing for Efficient Replication Complex Formation in Mosquito Cells
Bartholomeeusen, K., Utt, A., Coppens, S., Rausalu, K., Vereecken, K., Ariën, K. K., & Merits, A. (2018) · Journal of Virology · doi:10.1128/jvi.00152-18 · Cited by 47
Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT)
Jakobsen, J. E., Johansen, M., Schmidt, M. H., Dagnæs‐Hansen, F., Dam, K., Gunnarsson, A., Liu, Y., Kragh, P. M., Li, R., Holm, I. E., et al. (2012) · Transgenic Research · doi:10.1007/s11248-012-9671-6 · Cited by 44
Mammalian synthetic biology for studying the cell
Mathur, M., Xiang, J. S., & Smolke, C. D. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201611002 · Cited by 44
Calcium phosphate nanoneedle based gene delivery system for cancer genetic immunotherapy
Zhao, J., Chen, G., Pang, X., Zhang, P., Hou, X., Chen, P., Xie, Y.-W., He, C., Wang, Z., & Chen, Z. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120072 · Cited by 43
Self-citation
Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis
Park, N., Rim, Y. A., Jung, H., Kim, J., Yi, H., Kim, Y., Jang, Y., Jung, S. M., Lee, J. J., Kwok, S., et al. (2017) · Scientific Reports · doi:10.1038/srep39593 · Cited by 42
The march of pluripotent stem cells in cardiovascular regenerative medicine
Abou-Saleh, H., Zouein, F. A., EL‐Yazbi, A. F., Sanoudou, D., Raynaud, C. M., Rao, C., Pintus, G., Dehaini, H., & Eid, A. H. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0947-5 · Cited by 44
Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion
Gao, L., Xie, L., Long, X., Wang, Z., He, C.-Y., Chen, Z.-Y., Zhang, L., Nan, X., Lei, H., Liu, X., et al. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.01.094 · Cited by 41
Self-citation
Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site
Mun, J.-Y., Shin, K. K., Kwon, O., Lim, Y. T., & Oh, D. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.05.057 · Cited by 40
Delivery of a sebum modulator by an engineered skin microbe in mice
Knödlseder, N., Fábrega, M.-J., Santos‐Moreno, J., Manils, J., Toloza, L., Vilar, M. M., Fernández, C., Broadbent, K., Maruotti, J., Leménager, H., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-023-02072-4 · Cited by 41
A Dual Target-directed Agent against Interleukin-6 Receptor and Tumor Necrosis Factor α ameliorates experimental arthritis
Kim, Y., Yi, H., Jung, H., Rim, Y. A., Park, N., Kim, J., Jung, S. M., Park, S., Park, Y. W., & Ju, J. H. (2016) · Scientific Reports · doi:10.1038/srep20150 · Cited by 39
An update on ACE2 amplification and its therapeutic potential
Márquez, A., Wysocki, J. A., Pandit, J. A., & BATLLE, D. C. (2020) · Acta Physiologica · doi:10.1111/apha.13513 · Cited by 40
Bacteriophage recombination systems and biotechnical applications
Nafissi, N., & Slavcev, R. A. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5512-2 · Cited by 38
From scaffold to structure: the synthetic production of cell derived extracellular matrix for liver tissue engineering
Grant, R., Hay, D. C., & Callanan, A. (2018) · Biomedical Physics & Engineering Express · doi:10.1088/2057-1976/aacbe1 · Cited by 37
Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors
Williams, J. A., & Paez, P. A. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.04.003 · Cited by 35
DNA-Based Nonviral Gene Therapy─Challenging but Promising
Guan, X., Pei, Y., & Song, J. (2024) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.3c00907 · Cited by 35
Macrophage-specific in vivo RNA editing promotes phagocytosis and antitumor immunity in mice
Chen, Y., Chen, X., Zhang, Y., Wang, M., Yang, M., Wang, R. Y., Yan, X., Shao, S., Xin, H., Hu, Q., et al. (2025) · Science Translational Medicine · doi:10.1126/scitranslmed.adl5800 · Cited by 36
Efficient Sleeping Beauty DNA Transposition From DNA Minicircles
Sharma, N., Cai, Y., Bak, R. O., Jakobsen, M. R., Schrøder, L. D., & Mikkelsen, J. G. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.1 · Cited by 34
Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression
Stenler, S., Wiklander, O. P. B., Tejedor, M. B., Turunen, J. J., Nordin, J. Z., Hallengärd, D., Wahrén, B., Andaloussi, S. E., Rutland, M. W., Smith, C. E., et al. (2014) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.67 · Cited by 34
DNA Ministrings: Highly Safe and Effective Gene Delivery Vectors
Nafissi, N., Alqawlaq, S., Lee, E. A., Földvári, M., Spagnuolo, P. A., & Slavcev, R. A. (2014) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2014.16 · Cited by 34
Improved Minicircle DNA Biosynthesis for Gene Therapy Applications
Gaspar, V. M., Maia, C. J., Queiroz, J. A., Pichon, C., Correia, I. J., & de Sousa, F. P. (2013) · Human Gene Therapy Methods · doi:10.1089/hgtb.2013.020 · Cited by 34
Towards effective non-viral gene delivery vector
Šimčíková, M., Prather, K. L. J., Prazeres, D. M. F., & Monteiro, G. A. (2015) · Biotechnology and Genetic Engineering Reviews · doi:10.1080/02648725.2016.1178011 · Cited by 33
Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury
Florian, M., Wang, J.-P., Deng, Y., Souza‐Moreira, L., Stewart, D. J., & Mei, S. H. J. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02245-5 · Cited by 33

Authors: Kay, M. A., He, C.-Y., & Chen, Z.-Y.
Affiliations: Stanford Medicine (US)
Corresponding author: Kay, Mark A
ORCID: Kay, Mark A
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2010-11-21
Volume: 28
Issue: 12
Pages: 1287-1289
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.1708
PMC: PMC4144359
OpenAlex: W2079903460
Cited by: 329
References: 18
Citations by year: 2026: 12 · 2025: 20 · 2024: 21 · 2023: 20 · 2022: 17 · 2021: 28 · 2020: 20 · 2019: 22 · 2018: 19 · 2017: 17 · 2016: 28 · 2015: 24 · 2014: 31 · 2013: 23 · 2012: 16
FWCI: 6.51
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Heart, Lung, and Blood Institute (HL064274, R01HL064274)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords: transgene expression, gene transfer
MeSH terms: Biotechnology, Cloning, Molecular, DNA, Circular, Escherichia coli, Genetic Vectors, Plasmids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A robust system for production of minicircle DNA vectors

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks