A robust system for production of minicircle DNA vectors

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902

Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

Cherepanov, P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,952

An efficient recombination system for chromosome engineering in Escherichia coli

Yu, D., Ellis, H. M., Lee, E.-C., Jenkins, N. A., Copeland, N. G., & Court, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.100127597 · Cited by 1,688

A nonviral minicircle vector for deriving human iPS cells

Jia, F., Wilson, K. D., Sun, N., Gupta, D. M., Huang, M., Li, Z., Panetta, N. J., Chen, Z. Y., Robbins, R. C., Kay, M. A., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1426 · Cited by 734

Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivo

Chen, Z.-Y., He, C.-Y., Ehrhardt, A., & Kay, M. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00168-0 · Cited by 463

In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family

Thorpe, H. M., & Smith, M. C. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5505 · Cited by 434

Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter

Khlebnikov, A., Keasling, J. D., Wanner, B. L., Skaug, T., & Datsenko, K. A. (2001) · Microbiology · doi:10.1099/00221287-147-12-3241 · Cited by 289

A new DNA vehicle for nonviral gene delivery: supercoiled minicircle

Darquet, A.-M., Cameron, B., Wils, P., Scherman, D., & Crouzet, J. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300540 · Cited by 250

The Inhibitory Role of CpG Immunostimulatory Motifs in Cationic Lipid Vector-Mediated Transgene Expression in Vivo

Tan, Y., Li, S., Pitt, B. R., & Huang, L. (1999) · Human Gene Therapy · doi:10.1089/10430349950017149 · Cited by 236

Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

Chen, Z.-Y., He, C.-Y., & Kay, M. A. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.126 · Cited by 180

Precise kilobase-scale genomic insertions in mammalian cells using PASTE

Fell, C. W., Schmitt-Ulms, C., Tagliaferri, D. V., Gootenberg, J. S., & Abudayyeh, O. O. (2024) · Nature Protocols · doi:10.1038/s41596-024-01090-z · Cited by 8

Quantum pBac : An effective, high‐capacity piggyBac ‐ based gene integration vector system for unlocking gene therapy potential

Hua, W., Hsu, J. C., Chen, Y., Chang, P. S., Wen, K. K., Wang, P., Yang, W., Shen, C., Yu, Y., Chen, Y., et al. (2023) · The FASEB Journal · doi:10.1096/fj.202201654r · Cited by 8

Circle-seq based method for eccDNA synthesis and its application as a canonical promoter independent vector for robust microRNA overexpression

Yu, J., Zhang, H., Han, P., Jiang, X., Li, J., Li, B., Yang, S., He, C., Mao, S., Dang, Y., et al. (2023) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2023.12.019 · Cited by 8

Evaluation of DNA Vaccine Candidates against Foot-and-Mouth Disease Virus in Cattle

Puckette, M. C., Clark, B. A., BARRERA, J. D. C., Neilan, J. G., & Rasmussen, M. V. (2023) · Vaccines · doi:10.3390/vaccines11020386 · Cited by 8

Identification of sequences common to more than one therapeutic target to treat complex diseases: simulating the high variance in sequence interactivity evolved to modulate robust phenotypes

Varela, M. A. (2015) · BMC Genomics · doi:10.1186/s12864-015-1727-6 · Cited by 8

Reprogramming for cardiac regeneration

Raynaud, C. M., Ahmad, F. S., Allouba, M., Abou-Saleh, H., Lui, K. O., & Yacoub, M. H. (2014) · Global Cardiology Science and Practice · doi:10.5339/gcsp.2014.44 · Cited by 8

A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells

Delaney, A. M., Adams, C. F., Fernandes, A. R., al-Shakli, A. F., Sen, J., Carwardine, D. R., Granger, N. P., & Chari, D. M. (2017) · Nanoscale · doi:10.1039/c7nr00811b · Cited by 8

Minicircles for a two-step blood biomarker and PET imaging early cancer detection strategy

Robinson, E. R., Gowrishankar, G., D’Souza, A. L., Kheirolomoom, A., Haywood, T., Hori, S. S., Chuang, H., Zeng, Y., Tumbale, S. K., Aalipour, A., et al. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.05.026 · Cited by 8

Harnessing extracellular vesicles for stabilized and functional IL-10 delivery in macrophage immunomodulation

Saleh, N. A., Gagea, M. A., Vitija, X., Kilangodi, S., Zarea, A. A., Janovic, T., Schmidt, J. C., Deng, C. X., & Kanada, M. (2025) · Extracellular Vesicle · doi:10.1016/j.vesic.2025.100102 · Cited by 7

Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells

Shi, Y., Kopparapu, N., Ohler, L. S., & Dickinson, D. J. (2023) · Development · doi:10.1242/dev.201367 · Cited by 7

Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit

Yang, P., Yang, J., Ting, L., Liu, Q., Yin, Y., Chen, D., & Yang, S. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00339 · Cited by 7

Cabozantinib inhibits HBV-RNA transcription by decreasing STAT3 binding to the enhancer region of cccDNA

Funato, K., Miyake, N., Sekiba, K., Miyakawa, Y., Seimiya, T., Shibata, C., Kishikawa, T., & Otsuka, M. (2023) · Hepatology Communications · doi:10.1097/hc9.0000000000000313 · Cited by 7

Effects of stepwise administration of osteoprotegerin and parathyroid hormone-related peptide DNA vectors on bone formation in ovariectomized rat model

Eom, Y. J., Kim, J.-W., Rim, Y. A., Lim, J., Jung, S. I., & Ju, J. H. (2024) · Scientific Reports · doi:10.1038/s41598-024-51957-0 · Cited by 7

CRISPR/Cas9-mediated genome editing: from basic research to gene therapy

Goel, R. (2024) · International Journal of Research in Medical Sciences · doi:10.18203/2320-6012.ijrms20241589 · Cited by 7

Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma

Chang, T.-Y., Chung, C.-Y., Chuang, W.-M., Li, L., Jeng, L., & Ma, W. (2014) · BioMed Research International · doi:10.1155/2014/156356 · Cited by 7

Augmenter of liver regeneration (ALR) restrains concanavalin A-induced hepatitis in mice

Mu, M., Zhang, Z., Cheng, Y., Liu, G., Chen, X., Wu, X. T., Zhuang, C., Liu, B., Kong, X., & You, S. (2016) · International Immunopharmacology · doi:10.1016/j.intimp.2016.03.040 · Cited by 7

A novel approach for assessment of prostate cancer aggressiveness using survivin-driven tumour-activatable minicircles

Wang, T., Chen, Y., & Ronald, J. A. (2019) · Gene Therapy · doi:10.1038/s41434-019-0067-6 · Cited by 7

Biostimulatory Manganese-Doped Nanoneedle for MRI-Visual Bispecific Antibody Gene Delivery and Immunosuppression Reversal as a Cancer Immunotherapy Strategy

Cai, J., Jiang, S., Liao, J., Huiping, F., Chen, P., Shi, S., Huang, S., Chen, G., Xu, J., & Wang, Z. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2023.142242 · Cited by 7

Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids

Chung, K., & Booth, M. J. (2023) · Chemical Science · doi:10.1039/d3sc02761a · Cited by 7

Combination of Sleeping Beauty transposition and chemically induced dimerization selection for robust production of engineered cells

Kacherovsky, N. A., Harkey, M. A., Blau, C. A., Giachelli, C. M., & Pun, S. H. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks213 · Cited by 7

Enhanced gene disruption by programmable nucleases delivered by a minicircle vector

Dad, A.-B., Ramakrishna, S., Song, M., & Kim, S. (2014) · Gene Therapy · doi:10.1038/gt.2014.76 · Cited by 7

Variola Type IB DNA Topoisomerase: DNA Binding and Supercoil Unwinding Using Engineered DNA Minicircles

Anderson, B. G., & Stivers, J. T. (2014) · Biochemistry · doi:10.1021/bi500571q · Cited by 7

A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression

Makhija, H., Roy, S., Hoon, S. S., Ghadessy, F. J., Wong, D., Jaiswal, R., Campana, D., & Dröge, P. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky500 · Cited by 8

Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors

Porter, J. J., Ko, W., Sorensen, E. G., Cheung, Z., Thrasher, K., Couch, T. A., Gabell, J., Shwe, V., Hyatt, J., Licata, J. B., et al. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkag082 · Cited by 6

An advanced method for the small-scale production of high-quality minicircle DNA

Mitdank, H., Sama, S., Tröger, M., Testa, M. F., Ferrarese, M., Balestra, D., Pinotti, M., & Weng, A. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.120830 · Cited by 6

Anticancer effects of a single intramuscular dose of a minicircle DNA vector expressing anti-CD3/CD20 in a xenograft mouse model

Pang, X., Chen, G., Huang, P., Zhang, P., Liu, J., Hou, X., He, C., Chen, P., Xie, Y.-W., Zhao, J., et al. (2022) · Molecular Therapy — Oncolytics · doi:10.1016/j.omto.2022.02.014 · Cited by 6

A novel strategy for conditional gene knockout based on ΦC31 integrase and Gal4/UAS system in Drosophila

Ou, H., & Lei, T. (2013) · IUBMB Life · doi:10.1002/iub.1119 · Cited by 6

Minicircle DNA vector expressing interferon-lambda-3 inhibits hepatitis B virus replication and expression in hepatocyte-derived cell line

Guo, X., Chen, D., Cai, Q., Huang, Z., Xu, W., Peng, L., & Chen, P. (2020) · BMC Molecular and Cell Biology · doi:10.1186/s12860-020-00250-9 · Cited by 6

Evaluation of BMP-2 Minicircle DNA for Enhanced Bone Engineering and Regeneration

Zimmermann, A., Hercher, D., Regner, B., Frischer, A., Sperger, S., Redl, H. R., & Hacobian, A. (2020) · Current Gene Therapy · doi:10.2174/1566523220666200427121350 · Cited by 6

Suicide nanoplasmids coding for ribosome-inactivating proteins

Mitdank, H., Tröger, M., Sonntag, A., Shirazi, N. A., Woith, E., Fuchs, H., Kobelt, D., Walther, W., & Weng, A. (2021) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2021.106107 · Cited by 6

Reactivation of γ-globin expression using a minicircle DNA system to treat β-thalassemia

Ma, S., Gao, X.-X., Zhou, G., Zhang, H., Yang, J.-M., Wang, W., Song, X., Chen, H., & Lu, D. (2022) · Gene · doi:10.1016/j.gene.2022.146289 · Cited by 6

Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses

Stenler, S., Lundin, K. E., Hansen, L. F., Petkov, S., Mozafari, N., Isaguliants, M. G., Blomberg, P., Smith, C. I. E., Goldenberg, D. M., Chang, C., et al. (2017) · Human Vaccines & Immunotherapeutics · doi:10.1080/21645515.2017.1338546 · Cited by 6

The synthesis of amphiphilic polyethyleneimine/calcium phosphate composites for bispecific T-cell engager based immunogene therapy

Chen, P., Liu, Y., Zhao, J., Pang, X., Zhang, P., Hou, X., Chen, P., He, C., Wang, Z., & Chen, Z.-Y. (2018) · Biomaterials Science · doi:10.1039/c7bm01143a · Cited by 6

“Direct” and “Indirect” Effects of Histone Modifications: Modulation of Sterical Bulk as a Novel Source of Functionality

Krajewski, W. A. (2019) · BioEssays · doi:10.1002/bies.201900136 · Cited by 6

Construction of a new minicircle DNA carrying an enhanced green florescent protein reporter gene for efficient expression into mammalian cell lines

Ata-abadi, N. S., Dormiani, K., Khazaie, Y., Ghaedi, K., Forouzanfar, M., Lachinani, L., Rezaei, N., Kiani‐Esfahani, A., & Nasr‐Esfahani, M. H. (2015) · Molecular Biology Reports · doi:10.1007/s11033-015-3864-z · Cited by 6

Supercoiled DNA recognition and cleavage control in topoisomerase VI

Richman, D. E., Wendorff, T. J., Rashid, F., Beck, C., Yan, Q., Johnson, H. R., Eckerty, R. A., Fogg, J. M., Baker, M. L., Zechiedrich, L., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-69491-0 · Cited by 5

Viral vectors for gene delivery to the central nervous system

Ingusci, S., Hall, B. L., Goins, W. F., Cohen, J. B., & Glorioso, J. C. (2024) · Handbook of clinical neurology · doi:10.1016/b978-0-323-90120-8.00001-0 · Cited by 7

Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells

Oliynyk, R. T., Mahas, A., Karpinski, E., & Church, G. M. (2025) · Communications Biology · doi:10.1038/s42003-025-09157-7 · Cited by 5

Cancer Detection Using an Artificial Secretable MicroRNA Found in Blood and Urine

Shueng, P., Shih, K., Gambhir, S. S., Kuo, D., & Chuang, H. (2022) · Biomedicines · doi:10.3390/biomedicines10030621 · Cited by 5

Advances in Genetic Reprogramming: Prospects from Developmental Biology to Regenerative Medicine

Dhanjal, D. S., Singh, R., Sharma, V., Nepovimová, E., Adam, V., Kuča, K., & Chopra, C. (2023) · Current Medicinal Chemistry · doi:10.2174/0929867330666230503144619 · Cited by 5

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Wu, D. J., Dixit, N., Suzuki, É., Nguyen, T. H., Shin, H. S., Davis, J., Maverakis, E. M., & Adamopoulos, I. E. (2014) · Journal of Visualized Experiments · doi:10.3791/51810 · Cited by 5

Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate

Gaspar, V. M., Cruz, C., Queiroz, J. A., Pichon, C., Correia, I. J., & de Sousa, F. P. (2016) · Separation and Purification Technology · doi:10.1016/j.seppur.2016.10.054 · Cited by 5

Production of Minicircle DNA Vectors Using Site-Specific Recombinases

Ata-abadi, N. S., Rezaei, N., Dormiani, K., & Nasr‐Esfahani, M. H. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7169-5_20 · Cited by 5

Preparation of minicircle DNA vectors for gene knock-in to mammalian cells

Kawabe, Y., Hamaoka, M., Kuno, A., Yoshimura, Y., & Kamihira, M. (2025) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2025.05.005 · Cited by 4

Minicircle DNA vectors: A breakthrough in non-viral delivery of CRISPR base editors?

Kanada, M., & Gilad, A. A. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102275 · Cited by 4

A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma

Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., & Yu, Z. (2014) · Oncology Reports · doi:10.3892/or.2014.3486 · Cited by 4

CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells

van Essen, M. J., Riepsaame, J., & Jacob, J. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.0063 · Cited by 4

A robust knock-in approach using a minimal promoter and a minicircle

Keating, M. K., Hagle, R., Osorio-Méndez, D., Rodriguez, A., Almutawa, S. F. A. M. A., & Kang, J. (2023) · Developmental Biology · doi:10.1016/j.ydbio.2023.10.002 · Cited by 4

The molecular biology and HPV drug responsiveness of cynomolgus macaque papillomaviruses support their use in the development of a relevant in vivo model for antiviral drug testing

Tombak, E.-M., Männik, A., Burk, R. D., Grand, R. L., Ustav, E., & Ustav, M. (2019) · PLoS ONE · doi:10.1371/journal.pone.0211235 · Cited by 4

Increased Potential of Bone Formation with the Intravenous Injection of a Parathyroid Hormone-Related Protein Minicircle DNA Vector

Kim, J.-W., Park, N., Kang, J., Kim, Y., Jung, H., Rim, Y. A., & Ju, J. H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22169069 · Cited by 4

No evidence for restriction of Beta-HPV8 gene expression by epidermodysplasia verruciformis susceptibility genes CIB1, TMC6, or TMC8 in keratinocytes

Rehm, T. M., Parpoulas, C., Straub, E., Iftner, T., & Stubenrauch, F. (2025) · Tumour Virus Research · doi:10.1016/j.tvr.2025.200328 · Cited by 3

Host cell in vivo production of the synthetic drug anti–CD25/IL‐10 using minicircle vector

Lim, S. W., Shin, Y., Luo, K., Quan, Y., Ko, E. J., Chung, B. H., & Yang, C. W. (2019) · The FASEB Journal · doi:10.1096/fj.201900833r · Cited by 3

Selective Microvascular Tissue Transfection Using Minicircle DNA for Systemic Delivery of Human Coagulation Factor IX in a Rat Model Using a Therapeutic Flap

Than, P. A., Davis, C. R., Rennert, R. C., Morrison, S. D., Findlay, M. W., Kay, M. A., & Gurtner, G. C. (2021) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0000000000008630 · Cited by 3

Therapeutic effect of multiple functional minicircle vector encoding anti-CD25/IL-10/CXCR3 in allograft rejection model

Lim, S. W., Shin, Y., Cui, S., Ko, E. J., Yoo, S., Chung, B. H., & Yang, C. W. (2022) · The Korean Journal of Internal Medicine · doi:10.3904/kjim.2021.299 · Cited by 3

Large T antigen mediated target gene replication improves site-specific recombination efficiency

Wang, Z., Chen, C., & Ge, X. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1377167 · Cited by 3

Replicating Minicircles: Overcoming the Limitations of Transient and Stable Expression Systems

Nehlsen, K., Broll, S., Kandimalla, R., Heinz, N., Heine, M. A., Binius, S., Schambach, A., & Bode, J. (2013) · Wiley eBooks · doi:10.1002/9783527670420.ch8 · Cited by 3

High-Potency Polypeptide-based Inhibition of Enveloped-virus GlycoproteinsPreprint

Ma, J., Acevedo, A. C., & Wang, Q. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.04.05.438537 · Cited by 3

DNAJC9 Binds to and Enhances the Transcription of Hepatitis B Virus cccDNA by Recruiting Histone H3.3

Mao, T., Du, X., Li, Y., Zhou, Z., Li, D., Zheng, L., Zhang, T., Li, G., Yang, D., Chen, X., et al. (2025) · Journal of Medical Virology · doi:10.1002/jmv.70390 · Cited by 2

CRISPR-mediated proximity labeling unveils ABHD14B as a host factor to regulate HBV cccDNA transcriptional activity

Sekiba, K., Miyake, N., Miyakawa, Y., Shibata, C., Seimiya, T., Kishikawa, T., Fujishiro, M., & Otsuka, M. (2025) · Hepatology Communications · doi:10.1097/hc9.0000000000000757 · Cited by 2

Development of CpG‐Depleted CFTR Plasmid‐Based Nanoparticles for Nonviral Gene Therapy in Lung Cystic Fibrosis Disease

Qiu, B., Lorino, M., Li, Y., He, Z., Wang, X., Wang, W., & Lara‐Sáez, I. (2026) · The Journal of Gene Medicine · doi:10.1002/jgm.70087 · Cited by 2

Targeting papillomavirus infections: high-throughput screening reveals an effective inhibitor of cutaneous β-HPV types

Ibragimov, R., Babok, S., Lototskaja, E., Ivanova, L. B., Mikhailava, N., Zaikina, L., Piirsoo, M., & Piirsoo, A. (2025) · Journal of Virology · doi:10.1128/jvi.00918-25 · Cited by 2

Protocol for minicircle production for gene therapy without subsequent cleanup steps

Do, J., Verma, G., Maurice, Y., Abdumanobova, M., Deng, Z., Kalin, T. V., & Kalinichenko, V. V. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103982 · Cited by 2

Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production

Yuen, V. F. K., Yuan, D., Zhou, Y., Wang, H., Tan, D. Z. J., & Zhou, K. (2023) · Biochemical Engineering Journal · doi:10.1016/j.bej.2023.109007 · Cited by 2

Advancements in Non Viral Gene Therapy for Hemophilia

Fletcher, B. S. (2012) · Journal of Genetic Syndromes & Gene Therapy · doi:10.4172/2157-7412.s1-002 · Cited by 2

Recombination efficiency measurement by real-time PCR: A strategy to evaluate ParA-mediated minicircle production

Alves, C. P. A., Prazeres, D. M. F., & Monteiro, G. A. (2021) · Analytical Biochemistry · doi:10.1016/j.ab.2021.114285 · Cited by 2

Evaluation of DNA minicircles for delivery of adenine and cytosine base editors using activatable gene on “GO” reporter imaging systems

Evans, M. M., Liu, S., Krautner, J. S., Seguin, C., Leung, R., & Ronald, J. A. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102248 · Cited by 2

Biophysics Meets Gene Therapy: How Exploring Supercoiling-Dependent Structural Changes in Dna Led to The Development of Minivector Dna

Zechiedrich, L., & Fogg, J. M. (2019) · Technology & Innovation · doi:10.21300/20.4.2019.427 · Cited by 2

Development of viral & non-viral episomal vectors for gene therapy applications

Kymäläinen, H. (2012) · OpenGrey (Institut de l'Information Scientifique et Technique) · Cited by 2

High-Potency Polypeptide-based Interference for Coronavirus Spike GlycoproteinsPreprint

Wang, Q., Ma, J., & Acevedo, A. C. (2021) · Research Square · doi:10.21203/rs.3.rs-388285/v1 · Cited by 2

Quantum CART ( qCART ), a piggyBac-based system for development and production of virus-free multiplex CAR-T cell therapyPreprint

Chen, Y., Hua, W., Hsu, J. C., Chang, P. S., Wen, K. K., Huang, Y.-W., Tsai, J.-C., Kao, Y.-H., Wu, P.-H., Wang, P., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.05.03.490469 · Cited by 2

Treatment with Minicircle DNA Expressing a FGF23 Fragment in a Clinically relevant Mouse Model of X‐Linked Hypophosphatemic Rickets

Wu, H., Zhao, W., Chen, X., Wang, Y., Wei, S., Xiang, B., Shi, Y., Li, Z., Yao, Y., Xie, J., et al. (2025) · Advanced Science · doi:10.1002/advs.202508870 · Cited by 1

Targeted chondrogenic differentiation of human MSCs using niosomes for SOX9 gene delivery: comparison of minicircle and conventional plasmids

López-Seijas, J., Iglesias-Fente, A., Ramil-Bouzas, A., Paniagua-Barro, S., Fafián-Labora, J. A., & Rey‐Rico, A. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04867-5 · Cited by 1

Viral and Plasmid Transduction Systems: Methods to Modify Immune Cells for Cancer Immunotherapy

Xu, J. (2011) · Cited by 1

Gene Nanovector for Genome Therapy

Ma, D., & Xi, Z. (2023) · Springer eBooks · doi:10.1007/978-981-19-9776-1_60 · Cited by 1

The POU-HD TFs impede the replication efficiency of several human papillomavirus genomes

Kala, M., Babok, S., Mikhailava, N., Piirsoo, M., & Piirsoo, A. (2024) · Virology Journal · doi:10.1186/s12985-024-02334-w · Cited by 1

Gene Therapy and Gene Editing

Bazazzadegan, N., Hasanzad, M., & Abedini, S. S. (2024) · Springer eBooks · doi:10.1007/978-981-97-4974-4_9 · Cited by 1

Nonviral Delivery Systems for Gene Therapy for Retina and Posterior Segment Disease

del Pozo‐Rodríguez, A., Torrecilla, J., Rodríguez‐Gascón, A., & Solinís, M. Á. (2018) · Springer eBooks · doi:10.1007/978-3-319-95807-1_8 · Cited by 1

Liposome-Templated Hydrogel Nanoparticles for Targeted Delivery of CRISPR/Cas9 to Brain Tumors

Chen, Z., Chen, A. T., & Zhou, J. (2020) · Neuromethods · doi:10.1007/978-1-0716-1052-7_10 · Cited by 1

Coordinating the in vivo processes for minicircle production - a novel approach to large scale manufacturingPreprint

Mayrhofer, P., Jug, H., Štrancar, A., Paolo, A. D., Jost, L., Zimmermann, A., & Redl, H. R. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/300236 · Cited by 1

Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cellsPreprint

Webber, B. R., Johnson, M. J., Slipek, N. J., Lahr, W. S., DeFeo, A. P., Skeate, J. G., Qiu, X., Rathmann, B., Diers, M. D., Wick, B. J., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.11.12.468427 · Cited by 1

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Shi, S. J., Lin, Y., Ye, J. Z., Kwok, A., Want, K. Z., Cai, J. Z., Hu, M. M., Liu, A. Y., Li, K. J., Guo, B. Y., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.02.679775 · Cited by 1

Nonviral Gene Therapy—The Challenge of Mobilizing DNA

Mikkelsen, J. G. (2015) · Springer eBooks · doi:10.1007/978-1-4939-2389-2_4

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Denys, A., Thiolat, A., Boissier, M., & Natach, B. (2011) · InTech eBooks · doi:10.5772/19300

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Stenler, S. (2015) · Figshare

Development of new tools for the production of plasmid DNA biopharmaceuticals

Bower, D. M. (2012) · DSpace@MIT (Massachusetts Institute of Technology)

Synthetic immunosurveillance systems: Nanodevices to monitor physiological events

Woappi, Y., Jangiti, R., & Singh, O. V. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.04.056

Gene delivery to neural stem cells using minicircles and plasmids without CpG motifs

Reis, M. (2011) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)

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Skinner, N. (2013) · University of Minnesota Digital Conservancy (University of Minnesota)

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Higuti, E. (2016) · doi:10.11606/t.85.2016.tde-07032016-091035

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Fernandez, T. D. S., Mencalha, A. L., & Fernandez, C. D. S. (2016) · Stem cell biology and regenerative medicine · doi:10.1007/978-3-319-33270-3_7

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Chiew, M. Y. (2015)

Optimized Production and Purification of LCC DNA Minivectors for Applications in Gene Therapy and Vaccine Development

Sum, C. H. (2014) · UWSpace (University of Waterloo)

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Sainz-Ramos, M., Qtaish, N. A., Gallego, I., Villate-Beitia, I., López-Méndez, T. B., Puras, G., & Pedraz, J. L. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.79719

DNA Supercoil Unwinding by Variola Virus Type IB Topoisomerase is Accelerated by Superhelical Density and Hindered by Enzyme-DNA Interactions

Anderson, B. G. (2015)

Endogenous Reporter Systems for High-Throughput Functional Screening in Clear Cell Renal Cell Carcinoma

Zaini, M. N. (2019) · Apollo (University of Cambridge) · doi:10.17863/cam.41223

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Jiang, Y., Yang, G., & Wang, C. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0872-2_1

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Shah, H. N., Shen, A. H., Adem, S., Salhotra, A., Longaker, M. T., & Wan, D. C. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819376-1.00010-x

Increased Potential of Bone Formation With Intravenous Injection of Parathyroid Hormone-minicircle DNA VectorPreprint

Kim, J.-W., Park, N., Kang, J., Kim, Y., Jung, H., & Ju, J. H. (2020) · Research Square · doi:10.21203/rs.3.rs-88110/v1

Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy

Dietz, W. (2010) · University of Minnesota Digital Conservancy (University of Minnesota)

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Saavedra, F. J. M. (2013) · Cadernos de atención primaria

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Kay, M. A., He, C.-Y., & Chen, Z.-Y. (2016)

Technical Report Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

Chen, Z., He, C., & Kay, M. A. (2005)

141. Development of a One-Step Column Purification of Minicircle Vector DNA Devoid of Bacterial Sequences That Results in High Level Persistent Transgene Expression In Vivo

(2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.06.057 · Cited by 1

Production of Minicircle DNA Vectors Using Site-Specific Recombinases

Ata-abadi, N. S., Rezaei, N., Dormiani, K., & Nasr‐Esfahani, M. H. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7169-5_20 · Cited by 5

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Darquet, A.-M., Rangara, R., Kreiss, P., Schwartz, B., Naimi, S., Delaère, P., Crouzet, J., & Scherman, D. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300816 · Cited by 158