A robust system for production of minicircle DNA vectors

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902

Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

Cherepanov, P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,952

An efficient recombination system for chromosome engineering in Escherichia coli

Yu, D., Ellis, H. M., Lee, E.-C., Jenkins, N. A., Copeland, N. G., & Court, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.100127597 · Cited by 1,688

A nonviral minicircle vector for deriving human iPS cells

Jia, F., Wilson, K. D., Sun, N., Gupta, D. M., Huang, M., Li, Z., Panetta, N. J., Chen, Z. Y., Robbins, R. C., Kay, M. A., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1426 · Cited by 734

Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivo

Chen, Z.-Y., He, C.-Y., Ehrhardt, A., & Kay, M. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00168-0 · Cited by 463

In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family

Thorpe, H. M., & Smith, M. C. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5505 · Cited by 434

Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter

Khlebnikov, A., Keasling, J. D., Wanner, B. L., Skaug, T., & Datsenko, K. A. (2001) · Microbiology · doi:10.1099/00221287-147-12-3241 · Cited by 289

A new DNA vehicle for nonviral gene delivery: supercoiled minicircle

Darquet, A.-M., Cameron, B., Wils, P., Scherman, D., & Crouzet, J. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300540 · Cited by 250

The Inhibitory Role of CpG Immunostimulatory Motifs in Cationic Lipid Vector-Mediated Transgene Expression in Vivo

Tan, Y., Li, S., Pitt, B. R., & Huang, L. (1999) · Human Gene Therapy · doi:10.1089/10430349950017149 · Cited by 236

Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

Chen, Z.-Y., He, C.-Y., & Kay, M. A. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.126 · Cited by 180

News in brief

(2010) · Nature Methods · doi:10.1038/nmeth0111-13

Faculty Opinions recommendation of Niche stiffness underlies the ageing of central nervous system progenitor cells.

Appel, B. (2019) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.736419557.793564063

Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins

Sonntag, A., Mitdank, H., & Weng, A. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2441-8_8

Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase

Roy, S., Peter, S., & Dröge, P. (2022) · PLoS ONE · doi:10.1371/journal.pone.0270173

Gene Nanovector for Genome Therapy

Ma, D., & Xi, Z. (2022) · Springer eBooks · doi:10.1007/978-981-16-1313-5_60-1

Real-Time PCR Method for Assessment of ParA-Mediated Recombination Efficiency in Minicircle Production

Alves, C. P. A., Prazeres, D. M. F., & Monteiro, G. A. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3358-8_10

RAES：a rapid and high-fidelity method for artificial extrachromosomal circular DNA synthesis and its application as a bacterial DNA-free vector for robust microRNA overexpressionPreprint

Xiang, X., Yu, J., Haoran, Z., & Han, P. (2023) · Research Square · doi:10.21203/rs.3.rs-3243746/v1

Site-Specific Integration by Circular Donor Improves CRISPR/Cas9-Mediated Homologous Recombination in Human Cell Lines

Liu, Z., Zhao, Y. M., Wu, S., Qi, S., Qiu, Y., & Lian, Z. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252011320

A strategy for genome-wide seamless tagging of human protein-coding genesPreprint

González, L., Karimi, M., Castellanos-Girouard, X., Saber, M. M., Stifani, N., Gauthier, L., Serohijos, A. W. R., & Michnick, S. W. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.03.04.641506

Ministring DNA (msDNA): a novel linear covalently-closed DNA with enhanced stability for gene and cell therapy applications

Hung, W. K., Chandrasekaran, S., Zaslaver, O., Ming, Z., Lam, J., Hersch, S. J., Mokarami, P., Slavcev, R. A., & Nafissi, N. (2025) · Scientific Reports · doi:10.1038/s41598-025-98730-5

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Johnson, M. J., DeFeo, A. P., Slipek, N. J., Folsom, T. D., Henley, T., Choudhry, M. S., Webber, B. R., & Moriarity, B. S. (2025) · Journal of Visualized Experiments · doi:10.3791/68150

Autologous Production: The Future of Sustainable Antibody Treatments

Bouaziz, S., Rouleux‐Bonnin, F., David, S., Alegria, G. C., & Velge-Roussel, F. (2025) · Human Gene Therapy · doi:10.1089/hum.2025.052

Additional file 1 of The POU-HD TFs impede the replication efficiency of several human papillomavirus genomes

Kala, M., Babok, S., Mikhailava, N., Piirsoo, M., & Piirsoo, A. (2024) · Figshare · doi:10.6084/m9.figshare.25347143

Additional file 1 of Short cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system

Wang, X., Cai, C., Lv, W., Chen, K., Li, J., Liao, K., Zhang, Y., Huang, H., Lin, Y., Rong, Z., et al. (2024) · Figshare · doi:10.6084/m9.figshare.25902293

Additional file 2 of The POU-HD TFs impede the replication efficiency of several human papillomavirus genomes

Kala, M., Babok, S., Mikhailava, N., Piirsoo, M., & Piirsoo, A. (2024) · Figshare · doi:10.6084/m9.figshare.25347279

Advancing knock-in approaches for robust genome editing in zebrafish

Rodriguez, A., Beezley, E. G., Manna, S., Silaban, I. M., Almutawa, S. F. A. M. A., Cao, S., Ahmed, H., Guyer, M. E., Baker, S., Richards, M. P., et al. (2026) · Biology Open · doi:10.1242/bio.062472

Dual-biomarkers encoding tumor-activatable minicircles with scaffold/matrix attachment region motif for sensitive and sustained blood and urine-based cancer detection

Chuang, H., Lin, W.-C., Kao, Y. J., Chiang, C.-H., Chan, C. T., D’Souza, A. L., Gambhir, S. S., & Hori, S. S. (2026) · Cancer Cell International · doi:10.1186/s12935-026-04241-2

Self-circularized Overhang-based Unrecombined Products (SOUP): a rapid in vitro method for generating backbone-free expression cassettes

Cepleanu-Pascu, I. A., Popa, M. A., Cimponeriu, D., & Stoica, I. (2026) · BioTechniques · doi:10.1080/07366205.2026.2645351

Implication des réarrangements génomiques du polyomavirus JC dans la leucoencéphalopathie multifocale progressive

L’Honneur, A.-S. (2019) · doi:10.70675/0e724b28z7d2cz426ezad24zb4924594f25b

Non-viral gene therapy for hemophilia A: long-term outcomes of minicircle FVIII delivery in a mouse model

Kao, Y., Yen, C., Chang, G. R., Chiang, H.-T., Chen, J., Sufian, M., Chen, I.-C., Yang, S., & Chen, C. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1742144

Development of a gene-activated scaffold using niosomes and minicircle DNA as a novel non-viral delivery platform to enhance cartilage repair

López-Seijas, J., Iglesias-Fente, A., Intini, C., Dobricic, M., O’Brien, F. J., & Rey‐Rico, A. (2026) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2026.115909

Minicircle DNA Vaccines: Overcoming Delivery and Expression Barriers in Next-Generation Immunization

Alduhaymi, I. S., Majrashi, M. A., Alradwan, I. A., Alagrafi, F., Altammami, M. A., Aldossary, A. M., Almughem, F. A., Alshehri, A. A., Fallatah, M., Fayez, N. A., et al. (2026) · Vaccines · doi:10.3390/vaccines14070563

Engineering Piezo1‐Mediated Calcium Signalling for Enhanced Extracellular Vesicle Biogenesis and Cargo Loading

Saleh, N. A., Shafiq, R., Cicerone, A. P., Kilangodi, S., Hartwig, H., Mias, G. I., Lu, G., Huh, J. R., Li, W., Deng, C. X., et al. (2026) · Journal of Extracellular Biology · doi:10.1002/jex2.70182

Programmable enzymes for targeted gene insertion

Lampe, G. D., King, R. T., & Sternberg, S. H. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-026-01002-9

Validated chromatographic method for mcDNA quantification in complex samples

Valente, A. I., Valente, J. V., Ferreira, P. L., Pedro, A. Q., Tavares, A. P. M., de Sousa, F. P., & Freire, M. G. (2026) · Journal of Chromatography A · doi:10.1016/j.chroma.2026.467552

In vivo frataxin expression of a triplex purified minicircle

Mozafari, N., Milagres, S., Cuellar, R., Lundin, K. E., Fredriks, M., Ho, T.-T., Azam, M. G., Napierala, J. S., Напиерала, М., Wengel, J., et al. (2026) · Gene Therapy · doi:10.1038/s41434-026-00653-x

A Simple And Rapid Minicircle DNA Vector Manufacturing System

Kay, M. A., He, C.-Y., & Chen, Z.-Y. (2016)

Technical Report Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

Chen, Z., He, C., & Kay, M. A. (2005)

141. Development of a One-Step Column Purification of Minicircle Vector DNA Devoid of Bacterial Sequences That Results in High Level Persistent Transgene Expression In Vivo

(2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.06.057 · Cited by 1

Production of Minicircle DNA Vectors Using Site-Specific Recombinases

Ata-abadi, N. S., Rezaei, N., Dormiani, K., & Nasr‐Esfahani, M. H. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7169-5_20 · Cited by 5

Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

Darquet, A.-M., Rangara, R., Kreiss, P., Schwartz, B., Naimi, S., Delaère, P., Crouzet, J., & Scherman, D. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300816 · Cited by 158