The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
Open accessAbstract
Nanopore technologies are being developed for fast and direct sequencing of single DNA molecules through detection of ionic current modulations as DNA passes through a pore's constriction. Here we demonstrate the ability to resolve changes in current that correspond to a known DNA sequence by combining the high sensitivity of a mutated form of the protein pore Mycobacterium smegmatis porin A (MspA) with phi29 DNA polymerase (DNAP), which controls the rate of DNA translocation through the pore. As phi29 DNAP synthesizes DNA and functions like a motor to pull a single-stranded template through MspA, we observe well-resolved and reproducible ionic current levels with median durations of ∼28 ms and ionic current differences of up to 40 pA. Using six different DNA sequences with readable regions 42–53 nucleotides long, we record current traces that map to the known DNA sequences. With single-nucleotide resolution and DNA translocation control, this system integrates solutions to two long-standing hurdles to nanopore sequencing.
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Cites
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Self-citationSingle-molecule DNA detection with an engineered MspA protein nanopore
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The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
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Three decades of nanopore sequencing
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The Third Revolution in Sequencing Technology
van Dijk, E. L., Jaszczyszyn, Y., Naquin, D., & Thermes, C. (2018) · Trends in Genetics · doi:10.1016/j.tig.2018.05.008 · Cited by 1,057
Integrating Artificial Intelligence and Nanotechnology for Precision Cancer Medicine
Adir, O., Poley, M., Chen, G., Froim, S., Krinsky, N., Shklover, J., Shainsky‐Roitman, J., Lammers, T., & Schroeder, A. (2020) · Advanced Materials · doi:10.1002/adma.201901989 · Cited by 496
Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore
Ouldali, H., Sarthak, K., Ensslen, T., Piguet, F., Manivet, P., Pelta, J., Behrends, J. C., Aksimentiev, A., & Oukhaled, A. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0345-2 · Cited by 474
Metadata
- Authors
- Manrao, E. A., Derrington, I. M., Laszlo, A. H., Langford, K. W., Hopper, M. K., Gillgren, N., Pavlenok, M., Niederweis, M., & Gundlach, J. H.
- Venue
- Nature Biotechnology
- Published
- 2012-03-25
- Volume
- 30
- Issue
- 4
- Pages
- 349-353
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1993527536
- Cited by
- 965
- References
- 20
- FWCI
- 46.04
- Type
- journal article
- Language
- en
- Open access
- green
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- Manrao, E. A., Derrington, I. M., Laszlo, A. H., Langford, K. W., Hopper, M. K., Gillgren, N., Pavlenok, M., Niederweis, M., & Gundlach, J. H. (2012). Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase. Nature Biotechnology. https://doi.org/10.1038/nbt.2171
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