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Rapid nanopore discrimination between single polynucleotide molecules
Open accessAbstract
A variety of different DNA polymers were electrophoretically driven through the nanopore of an α-hemolysin channel in a lipid bilayer. Single-channel recording of the translocation duration and current flow during traversal of individual polynucleotides yielded a unique pattern of events for each of the several polymers tested. Statistical data derived from this pattern of events demonstrate that in several cases a nanopore can distinguish between polynucleotides of similar length and composition that differ only in sequence. Studies of temperature effects on the translocation process show that translocation duration scales as ∼ T −2 . A strong correlation exists between the temperature dependence of the event characteristics and the tendency of some polymers to form secondary structure. Because nanopores can rapidly discriminate and characterize unlabeled DNA molecules at low copy number, refinements of the experimental approach demonstrated here could eventually provide a low-cost high-throughput method of analyzing DNA polynucleotides.
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Cited by
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Deamer, D., Akeson, M., & Branton, D. (2016) · Nature Biotechnology · doi:10.1038/nbt.3423 · Cited by 1,292
Self-citationReading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
Manrao, E. A., Derrington, I. M., Laszlo, A. H., Langford, K. W., Hopper, M. K., Gillgren, N., Pavlenok, M., Niederweis, M., & Gundlach, J. H. (2012) · Nature Biotechnology · doi:10.1038/nbt.2171 · Cited by 965
Slowing DNA Translocation in a Solid-State Nanopore
Fologea, D., Uplinger, J., Thomas, B., McNabb, D. S., & Li, J. (2005) · Nano Letters · doi:10.1021/nl051063o · Cited by 567
Confinement effects on freezing and melting
Christenson, H. K. (2001) · Journal of Physics: Condensed Matter · doi:10.1088/0953-8984/13/11/201 · Cited by 567
Metadata
- Authors
- Meller, A., Nivon, L., Brandin, E., Golovchenko, J., & Branton, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-02
- Volume
- 97
- Issue
- 3
- Pages
- 1079-1084
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1971212710
- Cited by
- 937
- References
- 0
- FWCI
- 22.23
- Type
- journal article
- Language
- en
- Open access
- green
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- Meller, A., Nivon, L., Brandin, E., Golovchenko, J., & Branton, D. (2000). Rapid nanopore discrimination between single polynucleotide molecules. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.3.1079
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