The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization
Open accessAbstract
It is currently not possible to predict which epitopes will be recognized by T cells in different individuals. This is a barrier to the thorough analysis and understanding of T-cell responses after vaccination or infection. Here, by combining mass cytometry with combinatorial peptide–MHC tetramer staining, we have developed a method allowing the rapid and simultaneous identification and characterization of T cells specific for many epitopes. We use this to screen up to 109 different peptide–MHC tetramers in a single human blood sample, while still retaining at least 23 labels to analyze other markers of T-cell phenotype and function. Among 77 candidate rotavirus epitopes, we identified six T-cell epitopes restricted to human leukocyte antigen (HLA)-A*0201 in the blood of healthy individuals. T cells specific for epitopes in the rotavirus VP3 protein displayed a distinct phenotype and were present at high frequencies in intestinal epithelium. This approach should be useful for the comprehensive analysis of T-cell responses to infectious diseases or vaccines.
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Cites
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Cited by
Bystander CD8+ T cells are abundant and phenotypically distinct in human tumour infiltrates
Simoni, Y., Becht, E., Fehlings, M., Loh, C. Y., Koo, S.-L., Teng, K. W. W., Yeong, J. P. S., Nahar, R., Zhang, T., Kared, H., et al. (2018) · Nature · doi:10.1038/s41586-018-0130-2 · Cited by 1,370
Self-citationMass Cytometry: Single Cells, Many Features
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Human memory T cells: generation, compartmentalization and homeostasis
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CODEX multiplexed tissue imaging with DNA-conjugated antibodies
Black, S., Phillips, D., Hickey, J. W., Kennedy-Darling, J., Venkataraaman, V. G., Samusik, N., Goltsev, Y., Schürch, C. M., & Nolan, G. P. (2021) · Nature Protocols · doi:10.1038/s41596-021-00556-8 · Cited by 659
Metadata
- Authors
- Newell, E. W., Sigal, N., Nair, N., Kidd, B. A., Greenberg, H. B., & Davis, M. M.
- Venue
- Nature Biotechnology
- Published
- 2013-06-09
- Volume
- 31
- Issue
- 7
- Pages
- 623-629
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1977281284
- Cited by
- 302
- References
- 30
- FWCI
- 9.41
- Type
- journal article
- Language
- en
- Open access
- green
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