The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
MinION nanopore sequencing identifies the position and structure of a bacterial antibiotic resistance island
Open accessAbstract
Short-read, high-throughput sequencing technology cannot identify the chromosomal position of repetitive insertion sequences that typically flank horizontally acquired genes such as bacterial virulence genes and antibiotic resistance genes. The MinION nanopore sequencer can produce long sequencing reads on a device similar in size to a USB memory stick. Here we apply a MinION sequencer to resolve the structure and chromosomal insertion site of a composite antibiotic resistance island in Salmonella Typhi Haplotype 58. Nanopore sequencing data from a single 18-h run was used to create a scaffold for an assembly generated from short-read Illumina data. Our results demonstrate the potential of the MinION device in clinical laboratories to fully characterize the epidemic spread of bacterial pathogens.
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Cites
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Cited by
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Larsson, D. G. J., & Flach, C.-F. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00649-x · Cited by 3,164
Nanopore sequencing technology, bioinformatics and applications
Wang, Y., Zhao, Y., Bollas, A., Wang, Y., & Au, K. F. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01108-x · Cited by 1,753
The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
Jain, M., Olsen, H. E., Paten, B., & Akeson, M. (2016) · Genome Biology · doi:10.1186/s13059-016-1103-0 · Cited by 1,588
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“…to generate bacterial genome reference sequences [93, 94] and targeted amplicons [95,96], or used to generate a scaffold to map Illumina reads to [97,98,96], combining the ultra long read length of the nanopore technology and the high read depth and accuracy afforded by the short read sequencing.”
Metadata
- Authors
- Ashton, P. M., Nair, S., Dallman, T., Rubino, S., Rabsch, W., Mwaigwisya, S., Wain, J., & O'Grady, J.
- Venue
- Nature Biotechnology
- Published
- 2014-12-08
- Volume
- 33
- Issue
- 3
- Pages
- 296-300
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1991003805
- Cited by
- 461
- References
- 32
- FWCI
- 19.71
- Type
- journal article
- Language
- en
- Open access
- green
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