The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Abstract
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
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“More importantly, herbicide-resistant barley lines can be developed through base editing (Shimatani et al., 2017;Tian et al., 2018).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Shimatani et al., 2017
Citations
Cites
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool
Stemmer, M., Thumberger, T., del Sol Keyer, M., Wittbrodt, J., & Mateo, J. L. (2015) · PLoS ONE · doi:10.1371/journal.pone.0124633 · Cited by 1,088
CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants
Yang, L., Lü, L., Liu, H., Li, S., Feng, X., & Chen, L. (2014) · Molecular Plant · doi:10.1093/mp/ssu044 · Cited by 815
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Resistance to AHAS inhibitor herbicides: current understanding
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Sun, H., Uchii, S., Watanabe, S., & Ezura, H. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci251 · Cited by 476
Self-citationPrecise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System
Lu, Y., & Zhu, J. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.11.013 · Cited by 423
Rapid and efficientAgrobacterium-mediated transformation in rice
Toki, S. (1997) · Plant Molecular Biology Reporter · doi:10.1007/bf02772109 · Cited by 381
Efficient gene targeting by homologous recombination in rice
Terada, R., Urawa, H., Inagaki, Y., Tsugane, K., & Iida, S. (2002) · Nature Biotechnology · doi:10.1038/nbt737 · Cited by 327
Self-citationGeneration of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System
Li, J., Sun, Y., Du, J., Zhao, Y., & Xia, L. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.12.001 · Cited by 304
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
Mikami, M., Toki, S., & Endo, M. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0342-x · Cited by 297
Negative reciprocal interactions between gibberellin and cytokinin in tomato
Fleishon, S., Shani, E., Ori, N., & Weiss, D. W. (2011) · New Phytologist · doi:10.1111/j.1469-8137.2010.03616.x · Cited by 106
Genes that influence yield in tomato
Ariizumi, T., Shinozaki, Y., & Ezura, H. (2013) · Ikushugaku zasshi · doi:10.1270/jsbbs.63.3 · Cited by 105
Self-citationTerada, R., Asao, H., & Iida, S. (2004) · Plant Cell Reports · doi:10.1007/s00299-003-0752-0 · Cited by 64
Self-citationDang, T. T., Shimatani, Z., Kawano, Y., Terada, R., & Shimamoto, K. (2013) · Plant and Cell Physiology · doi:10.1093/pcp/pct147 · Cited by 25
Self-citationCharacterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice
Shimatani, Z., Takagi, K., Eun, C., Maekawa, M., Takahara, H., Hoshino, A., Qian, Q., Terada, R., Johzuka‐Hisatomi, Y., Iida, S., et al. (2009) · Molecular Genetics and Genomics · doi:10.1007/s00438-008-0410-x · Cited by 15
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Research works printed but not linked: 1
- Nishida, K. et al. Science 102, 553–563 (2016).
Cited by
Sorted by citations
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review
Raza, A., Razzaq, A., Mehmood, S. S., Zou, X., Zhang, X., Lv, Y., & Xu, J. (2019) · Plants · doi:10.3390/plants8020034 · Cited by 1,762
“Without any transformation of a gene, wheat has been developed with a low gluten level by using CRISPR/Cas9 technique [246].”
BackgroundInfluentialBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture
Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,696
Genome engineering for crop improvement and future agriculture
Gao, C. (2021) · Cell · doi:10.1016/j.cell.2021.01.005 · Cited by 1,070
Metadata
- Authors
- Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T., & Kondo, A.
- Affiliations
- Kobe University (JP)
- University of Tsukuba (JP)
- Meijo University (JP)
- Corresponding author
- Ezura, Hiroshi
- Nishida, Keiji
- Ariizumi, Tohru
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2017-03-27
- Volume
- 35
- Issue
- 5
- Pages
- 441-443
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2598674316
- Cited by
- 812
- References
- 17
- Citations by year
- 2026: 43 · 2025: 71 · 2024: 82 · 2023: 83 · 2022: 117 · 2021: 113 · 2020: 107 · 2019: 97 · 2018: 62 · 2017: 37
- FWCI
- 33.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (15K18647, 16K14654, 16K07560)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- base editing, CRISPR/Cas9, rice, tomato, point mutagenesis, herbicide resistance, single guide RNA, crop improvement
- MeSH terms
- Gene Editing, Cytidine Deaminase, Recombinant Fusion Proteins, Oryza, Mutagenesis, Site-Directed, Genes, Plant, Point Mutation, Solanum lycopersicum, DNA, Plant, Base Pairing, Plants, Genetically Modified, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Associated Proteins
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