Cited by 80RNA and protein synthesis mechanismsMicrobial Metabolic Engineering and BioproductionViral Infectious Diseases and Gene Expression in Insects

Digital-to-biological converter for on-demand production of biologics.

Boles, Kent S, Kannan, Krishna, Gill, John, Felderman, Martina, Gouvis, Heather, Hubby, Bolyn, Kamrud, Kurt I, Venter, J Craig +1 more·2017·Nature Biotechnology

Abstract

Manufacturing processes for biological molecules in the research laboratory have failed to keep pace with the rapid advances in automization and parellelization. We report the development of a digital-to-biological converter for fully automated, versatile and demand-based production of functional biologics starting from DNA sequence information. Specifically, DNA templates, RNA molecules, proteins and viral particles were produced in an automated fashion from digitally transmitted DNA sequences without human intervention.

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Crossref · 10.1038/nbt.3859

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Authors
Boles, K. S., Kannan, K., Gill, J., Felderman, M., Gouvis, H., Hubby, B., Kamrud, K. I., Venter, J. C., & Gibson, D. G.
Affiliations
Synthetic Genomics (United States) (US)
Corresponding author
Gibson, Daniel G
Venue
Nature Biotechnology
Published
2017-05-29
Volume
35
Issue
7
Pages
672-675
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
OpenAlex
W2620432775
Cited by
80
References
21
Citations by year
2026: 3 · 2025: 11 · 2024: 8 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 10 · 2019: 7 · 2018: 20 · 2017: 6
FWCI
4.81
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
DNA template, RNA synthesis, protein production, phage particles
MeSH terms
Biological Products, Biopolymers, Equipment Design, Genetic Engineering, Robotics, Equipment Failure Analysis, Synthetic Biology, High-Throughput Nucleotide Sequencing

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