Cited by 1,326Advanced Fluorescence Microscopy TechniquesSpectroscopy Techniques in Biomedical and Chemical ResearchCell Image Analysis Techniques

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms

Campagnola, Paul J, Loew, Leslie M·2003·Nature Biotechnology

DOI 10.1038/nbt894

Open this paper in CoChat — free account

Abstract

Although the nonlinear optical effect known as second-harmonic generation (SHG) has been recognized since the earliest days of laser physics and was demonstrated through a microscope over 25 years ago, only in the past few years has it begun to emerge as a viable microscope imaging contrast mechanism for visualization of cell and tissue structure and function. Only small modifications are required to equip a standard laser-scanning two-photon microscope for second-harmonic imaging microscopy (SHIM). Recent studies of the three-dimensional in vivo structures of well-ordered protein assemblies, such as collagen, microtubules and muscle myosin, are beginning to establish SHIM as a nondestructive imaging modality that holds promise for both basic research and clinical pathology. Thus far the best signals have been obtained in a transmitted light geometry that precludes in vivo measurements on large living animals. This drawback may be addressed through improvements in the collection of SHG signals via an epi-illumination microscope configuration. In addition, SHG signals from certain membrane-bound dyes have been shown to be highly sensitive to membrane potential. Although this indicates that SHIM may become a valuable tool for probing cell physiology, the small signal size would limit the number of photons that could be collected during the course of a fast action potential. Better dyes and optimized microscope optics could ultimately lead to the imaging of neuronal electrical a…

Integrity signals

Registry

Crossref · 10.1038/nbt894

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Zipfel, W. R., Williams, R. M., Christie, R. H., Nikitin, A. Y., Hyman, B. T., & Webb, W. W. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832308100 · Cited by 1,712
Three-Dimensional High-Resolution Second-Harmonic Generation Imaging of Endogenous Structural Proteins in Biological Tissues
Campagnola, P. J., Millard, A. C., Terasaki, M., Hoppe, P. E., Malone, C. J., & Mohler, W. A. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75414-3 · Cited by 922
Self-citation
Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
Zoumi, A., Yeh, A. T., & Tromberg, B. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172368799 · Cited by 872
Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
Brown, E. B., McKee, T. D., DiTomaso, E., Pluen, A., Seed, B., Boucher, Y., & Jain, R. K. (2003) · Nature Medicine · doi:10.1038/nm879 · Cited by 844
High-Resolution Nonlinear Optical Imaging of Live Cells by Second Harmonic Generation
Campagnola, P. J., Wei, M.-D., Lewis, A., & Loew, L. M. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77165-1 · Cited by 504
Self-citation
Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon
Freund, I., Deutsch, M., & Sprecher, A. (1986) · Biophysical Journal · doi:10.1016/s0006-3495(86)83510-x · Cited by 449
3-Dimensional imaging of collagen using second harmonic generation
Cox, G., Kable, E., Jones, A., Fraser, I., Manconi, F., & Gorrell, M. D. (2003) · Journal of Structural Biology · doi:10.1016/s1047-8477(02)00576-2 · Cited by 430
Polarization-Modulated Second Harmonic Generation in Collagen
Stoller, P. C., Reiser, K. M., Celliers, P. M., & Rubenchik, A. M. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75673-7 · Cited by 403
Membrane imaging by second-harmonic generation microscopy
Moreaux, L. C., Sandre, O., & Mertz, J. C. (2000) · Journal of the Optical Society of America B · doi:10.1364/josab.17.001685 · Cited by 346
Video Microscopy
Inoué, S. (1986) · Springer eBooks · doi:10.1007/978-1-4757-6925-8 · Cited by 343

Flagged first, then by citations

Show all 29 references

Research works printed but not linked: 3

Sheppard, C.J.R., Kompfner, R., Gannaway, J. & Walsh, D. Scanning harmonic optical microscope. IEEE J. Quantum Electron. 13E, 100D (1977).
Brown, E. et al. Dynamic imaging of collagen and its modulation in tumors in vivo using second harmonic generation. Nat. Med. 9, 796–800 (2003). · DOI
Huang, J.Y., Lewis, A. & Loew, L.M. Non-linear optical properties of potential sensitive styryl dyes. Biophys. J. 53, 665–670 (1988). · DOI

Sorted by citations

Items 801–900 of 1,000

Assessment of Extramammary Paget Disease by Two-Photon Microscopy
Hristu, R., Eftimie, L. G., Stanciu, S. G., Glogojeanu, R. R., Gheorghita, P., & Stanciu, G. A. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2022.839786 · Cited by 9
Solvent Polarity Governs Ultrafast Structural Dynamics: A Case Study of 4-Dimethylamino-4′-carbomethoxydiphenylacetylene
Barak, A., Dhiman, N., Sturm, F., Rauch, F., Lakshmanna, Y. A., Findlay, K. S., Beeby, A., Marder, T. B., & Umapathy, S. (2023) · The Journal of Physical Chemistry C · doi:10.1021/acs.jpcc.2c07691 · Cited by 9
Decrimping: The first stage of collagen thermal denaturation unraveled by in situ second-harmonic-generation imaging
Liao, C., Zhuo, Z.-Y., Yu, J., Tzeng, Y.-Y., Chu, S., Yu, S.-F., & Chao, P. G. (2011) · Applied Physics Letters · doi:10.1063/1.3578191 · Cited by 9
Chiral second‐harmonic generation from small organic molecules at surfaces
Persechini, L., & McGilp, J. F. (2012) · physica status solidi (b) · doi:10.1002/pssb.201100562 · Cited by 9
Tunable Multicolored Femtosecond Pulse Generation Using Cascaded Four-Wave Mixing in Bulk Materials
He, J., Liu, J., & Kobayashi, T. (2014) · Applied Sciences · doi:10.3390/app4030444 · Cited by 9
Nonlinear optical microscopy for visualizing dermal structural assembly in normal and pathological human dermis
Zhuo, S., Chen, J., Xie, S., Zheng, L., Hong, Z.-S., & Jiang, X. S. (2009) · Laser Physics Letters · doi:10.1002/lapl.200910069 · Cited by 9
The Use of Second-Order Susceptibility as Contrast Mechanism for Label-Free Imaging of Biological Tissue
Hu, P., Hsueh, C., Su, P.-J., Chen, W., Hovhannisyan, V., Chen, S., Tsai, T., & Dong, C. (2011) · IEEE Journal of Selected Topics in Quantum Electronics · doi:10.1109/jstqe.2011.2174619 · Cited by 9
Spherulites: onion-like Vesicles As Nanomedicines
Crauste–Manciet, S., Khawand, K., & Mignet, N. (2015) · Therapeutic Delivery · doi:10.4155/tde.14.81 · Cited by 9
Second harmonic generation imaging reveals a distinct organization of collagen fibrils in locations associated with cartilage growth
Finnøy, A., Olstad, K., & Lilledahl, M. B. (2016) · Connective Tissue Research · doi:10.1080/03008207.2016.1190348 · Cited by 9
Ultrafast adiabatic second harmonic generation
Dahan, A., Levanon, A., Katz, M., & Suchowski, H. (2017) · Journal of Physics Condensed Matter · doi:10.1088/1361-648x/29/8/084004 · Cited by 9
Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion
Eftimie, L. G., Padrez, Y., Golubewa, L., Rutkauskas, D., & Hristu, R. (2024) · Biomedical Optics Express · doi:10.1364/boe.523052 · Cited by 8
Microstructure-Informed Hyper-Viscoelastic Model Capturing Soft Tissue Tensile Behavior Across Large Deformations
Shi, L. T., & Myers, K. M. (2025) · Journal of the Mechanics and Physics of Solids · doi:10.1016/j.jmps.2025.106348 · Cited by 8
SHG Fiberscopy Assessment of Collagen Morphology and Its Potential for Breast Cancer Optical Histology
Hall, G., Liang, W., Bhujwalla, Z. M., & Li, X. D. (2024) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2024.3372629 · Cited by 8
Accelerating whole-sample polarization-resolved second harmonic generation imaging in mammary gland tissue via generative adversarial networks
Aghigh, A., Cardot, J., Mohammadi, M. S., Jargot, G., Ibrahim, H., Plante, I., & Légaré, F. (2024) · Biomedical Optics Express · doi:10.1364/boe.529779 · Cited by 8
On probing conformation of microtubules by second-harmonic generation
Sharoukhov, D., & Lim, H. (2015) · Journal of Modern Optics · doi:10.1080/09500340.2015.1080866 · Cited by 8
Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy
Li, C., Pastila, R. K., & Lin, C. P. (2015) · Journal of Innovative Optical Health Sciences · doi:10.1142/s1793545816400034 · Cited by 8
Elimination of imaging artifacts in second harmonic generation microscopy using interferometry
Pinsard, M., Schmeltz, M., van der Kolk, J. N., Patten, S. A., Ibrahim, H., Ramunno, L., Schanne‐Klein, M., & Légaré, F. (2019) · Biomedical Optics Express · doi:10.1364/boe.10.003938 · Cited by 8
Automated classification of breast cancer histologic grade using multiphoton microscopy and generative adversarial networks
Xi, G., Wang, Q., Zhan, H., Kang, D., Liu, Y., Luo, T., Xu, M., Kong, Q., Zheng, L., Chen, G., et al. (2022) · Journal of Physics D Applied Physics · doi:10.1088/1361-6463/aca104 · Cited by 8
The molecular basis of tumor metastasis and current approaches to decode targeted migration-promoting events in pediatric neuroblastoma
Corallo, D., Vecchia, M. D., Lazic, D., Taschner‐Mandl, S., Biffi, A., & Aveic, S. (2023) · Biochemical Pharmacology · doi:10.1016/j.bcp.2023.115696 · Cited by 9
Second Harmonic Imaging of Membrane Potential
Loew, L. M., & Lewis, A. (2015) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-17641-3_19 · Cited by 8
Self-citation
Tip-Enhanced Second Harmonic Generation From a Single NaNbO3 Nanocrystal
Chen, G., Wu, E., Ding, C., Xiao, Q., Ci, X., Liu, Y., Rong, Y., Wu, B., Qiu, J., & Zeng, H. (2015) · IEEE Journal of Selected Topics in Quantum Electronics · doi:10.1109/jstqe.2015.2413406 · Cited by 8
Second‐harmonic generation voltage imaging at subcellular resolution in rat hippocampal slices
Rama, S., Vetrivel, L., & Semyanov, A. V. (2010) · Journal of Biophotonics · doi:10.1002/jbio.201000073 · Cited by 8
Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage
Mansour, J. M., Lee, Z., & Welter, J. F. (2016) · Annals of Biomedical Engineering · doi:10.1007/s10439-015-1535-9 · Cited by 8
Imaging the pancreatic ECM
Venkatasubramanian, P. N. (2012) · Europe PMC (PubMed Central) · Cited by 8
Three‐photon‐resonance‐enhanced third‐harmonic generation for label‐free deep‐brain imaging: In search of a chemical contrast
Lanin, A. А., Chebotarev, A. S., Pochechuev, M. S., Kelmanson, I. V., Fedotov, A. B., Belousov, V. V., & Zheltikov, A. M. (2019) · Journal of Raman Spectroscopy · doi:10.1002/jrs.5566 · Cited by 8
Fundamentals of Optical Imaging
Lin, L., Jiang, P., Bao, Z., Pang, W., Ding, S., Yin, M., Li, P., & Gu, B. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-7627-0_1 · Cited by 8
Nomogram model combining macro and micro tumor-associated collagen signatures obtained from multiphoton images to predict the histologic grade in breast cancer
Xi, G., He, J., Kang, D., Xu, S., Guo, W., Fu, F., Liu, Y., Zheng, L., Qiu, L., Li, L., et al. (2021) · Biomedical Optics Express · doi:10.1364/boe.433281 · Cited by 8
Virtual spatial overlap modulation microscopy for resolution improvement
Kuo, W.-C., Shih, Y., Hsu, H., Cheng, Y.-H., Liao, Y., & Sun, C. (2013) · Optics Express · doi:10.1364/oe.21.030007 · Cited by 8
Dorsal Skin Fold Chamber for High Resolution Multiphoton Imaging
Li, F., Wang, C., Lin, S., Jee, S., Lo, W., & Dong, C. (2005) · Optical and Quantum Electronics · doi:10.1007/s11082-005-4223-4 · Cited by 8
Applying tattoo dye as a third-harmonic generation contrast agent for in vivo optical virtual biopsy of human skin
Tsai, M., Lin, C., Liao, Y., & Sun, C. (2013) · Journal of Biomedical Optics · doi:10.1117/1.jbo.18.2.026012 · Cited by 8
Sequential Multitrack Nonlinear Ex vivo Imaging of Esophageal Stroma Based on Backscattered Second‐harmonic Generation and Two‐photon Autofluorescence
Zhuo, S., Chen, J., Jiang, X., Luo, T., Chen, R., Xie, S., & Zou, Q. (2007) · Scanning · doi:10.1002/sca.20066 · Cited by 8
Label-Free Second Harmonic Generation Imaging of Cerebral Vascular Wall in Local Ischemia Mouse Model In Vivo
Sun, H., Wang, S., Chen, J., & Yu, H. (2022) · Neuroscience · doi:10.1016/j.neuroscience.2022.08.003 · Cited by 7
On the importance of simultaneous label-free multimodal nonlinear optical imaging for biomedical applications
la Cadena, A. D., Park, J., Shi, J., & Boppart, S. A. (2025) · APL Photonics · doi:10.1063/5.0289864 · Cited by 7
Tissue Imaging and Quantification Relying on Endogenous Contrast
Liu, Z., Meng, J., Quinn, K. P., & Georgakoudi, I. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-7627-0_12 · Cited by 6
Laser pulse induced second- and third-harmonic generation of gold nanorods with real-time time-dependent density functional tight binding (RT-TDDFTB) method
Giri, S. K., & Schatz, G. C. (2024) · The Journal of Chemical Physics · doi:10.1063/5.0216887 · Cited by 7
An efficient and cost-effective methods framework for performing biomechanical analysis on cadaveric skeletal muscle
Byrd, J. J., & Snow, E. L. (2024) · Translational Research in Anatomy · doi:10.1016/j.tria.2024.100335 · Cited by 7
Gauge-origin independent calculations of electric-field-induced second-harmonic generation circular intensity difference using London atomic orbitals
Anelli, M., Ringholm, M., & Ruud, K. (2016) · Molecular Physics · doi:10.1080/00268976.2016.1249979 · Cited by 7
Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy
Fernández, A., Grüner-Nielsen, L., Andreana, M., Stadler, M., Kirchberger, S., Sturtzel, C., Distel, M., Zhu, L., Kautek, W., Leitgeb, R. A., et al. (2017) · Biomedical Optics Express · doi:10.1364/boe.8.003526 · Cited by 7
Tunable Grain Orientation of Chalcogenide Film and Its Application for Second Harmonic Generation
Meng, Y., Behera, J. K., Wang, S., Jiang, M., Lin, J., Wei, J., Wang, Y., Cao, T., & Long, Y. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c05082 · Cited by 7
Prognostic value of tumor stromal collagen features in patients with hepatocellular carcinoma revealed by second-harmonic generation microscopy
Lin, L., Chen, G., Chen, Z., Lu, J., Zhu, W., Zhong, J., Peng, F., & Huang, A. (2020) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2020.104513 · Cited by 7
Leaving the Limits of Linearity for Light Microscopy
Blake, M. J., Colon, B. A., & Calhoun, T. R. (2020) · The Journal of Physical Chemistry C · doi:10.1021/acs.jpcc.0c07501 · Cited by 7
Multimodal nonlinear-optical imaging of nucleoli
Pochechuev, M. S., Lanin, A. A., Kelmanson, I. V., Chebotarev, A. S., Fetisova, E. S., Bilan, D. S., Shevchenko, E. K., Ivanov, A. A., Fedotov, A. B., Belousov, V. V., et al. (2021) · Optics Letters · doi:10.1364/ol.416300 · Cited by 7
Label-free and noninvasive method for assessing the metabolic status in type 2 diabetic rats with myocardium diastolic dysfunction
Guo, K., Wu, J., Kong, Y., Zhou, L., Li, W., Fei, Y., Ma, J., & Mi, L. (2020) · Biomedical Optics Express · doi:10.1364/boe.413347 · Cited by 7
Region-resolved multi-omics of the mouse eye
Hang, X., Zhang, B., Wang, Y., Xu, N., Zhang, F., Luo, R., Ji, M., & Ding, C. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112121 · Cited by 7
Interfacial Second-Harmonic Generation via Superposition of Symmetries in a Double-Resonance-Enhanced Plasmonic Nanocavity
Chen, H., Jiang, Z., Kang, B., Guo, L., Yan, L., Fu, Z., & Zhang, Z. (2024) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.4c00548 · Cited by 7
Molecular Spectroscopic Imaging Using a White-Light Laser Source
Kano, H. (2010) · Bulletin of the Chemical Society of Japan · doi:10.1246/bcsj.20100004 · Cited by 7
Molecular Conformation in Organic Films from Quantum Chemistry ab Initio Calculations and Second Harmonic Spectroscopy
Macovez, R., López, N., Mariano, M., Maymó, M., & Martorell, J. (2012) · The Journal of Physical Chemistry C · doi:10.1021/jp3075018 · Cited by 7
Multiphoton microscopy imaging of developing tooth germs
Pan, P.-Y., Chen, R., Ting, C.-L., Chen, W., Dong, C., & Chen, M. (2012) · Journal of the Formosan Medical Association · doi:10.1016/j.jfma.2012.03.016 · Cited by 7
Two-photon laser scanning microscopy on native cartilage and collagen-membranes for tissue-engineering
Martini, J., Tönsing, K., Dickob, M., Schade, R., Liefeith, K., & Anselmetti, D. (2006) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.641430 · Cited by 7
Poly-l-arginine modifications alter the organization and secretion of collagen in SKH1-E mice
Boddupalli, A., Akilbekova, D., & Bratlie, K. M. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110143 · Cited by 7
Fluorescence and Multiphoton Imaging for Tissue Characterization of a Model of Postmenopausal Ovarian Cancer
Sawyer, T. W., Koevary, J. W., Howard, C. C., Austin, O. J., Rice, P. F. S., Hutchens, G. V., Chambers, S. K., Connolly, D. C., & Barton, J. K. (2020) · Lasers in Surgery and Medicine · doi:10.1002/lsm.23251 · Cited by 7
Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
Zyablitskaya, M., Munteanu, E. L., Nagasaki, T., & Paik, D. C. (2018) · Journal of Visualized Experiments · doi:10.3791/56385 · Cited by 7
Nonlinear‐optical stain‐free stereoimaging of astrocytes and gliovascular interfaces
Lanin, A. А., Pochechuev, M. S., Chebotarev, A. S., Kelmanson, I. V., Belousov, V. V., & Zheltikov, A. M. (2019) · Journal of Biophotonics · doi:10.1002/jbio.201800432 · Cited by 7
Imaging collagen remodeling and sensing transplanted autologous fibroblast metabolism in mouse dermis using multimode nonlinear optical imaging
Zhuo, S., Chen, J., Cao, N., Jiang, X., Xie, S., & Xiong, S. (2008) · Physics in Medicine and Biology · doi:10.1088/0031-9155/53/12/017 · Cited by 7
Open quantum systems theory of ultraweak ultraviolet photon emissions: Revisiting Gurwitsch's onion experiment as a prototype for quantum biology
Babcock, N. S. (2024) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2024.11.030 · Cited by 8
Step‐Ladder Bioprinting to Align Collagen Fibers for Anisotropic Tissue Fabrication
Namlı, İ., Singh, Y. P., Gupta, D., Rizvi, S. H. A., Yilmaz, Y. O., Robazzi, J. V. S., Sarıkaya, M. D., Baykara, M., & Ozbolat, I. T. (2026) · Small · doi:10.1002/smll.202510498 · Cited by 6
Quantification methodologies on organization and morphology features of fiber-like structures: A review
Wang, C., Meng, J., Qian, S., Zhou, L., Jiang, S., Jiang, R., Zhan, H., Fang, X., Liu, Y., Ding, Z., et al. (2022) · Journal of Innovative Optical Health Sciences · doi:10.1142/s1793545822300129 · Cited by 6
Distribution of smooth muscle actin and collagen in superficial peritoneal endometriotic lesions varies from the surrounding microenvironment
Colgrave, E. M., Keast, J. R., Nowell, C. J., Healey, M., Rogers, P. A. W., Holdsworth‐Carson, S. J., & Girling, J. E. (2023) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2023.103610 · Cited by 6
Live-cell imaging of human liver fibrosis using hepatic micro-organoids
Guan, Y., Fang, Z., Hu, A., Roberts, S., Wang, M., Ren, W., Johansson, P. K., Heilshorn, S. C., Enejder, A., & Peltz, G. (2025) · JCI Insight · doi:10.1172/jci.insight.187099 · Cited by 6
Advances in Imaging of Traumatic Nerve Injuries
Graesser, E. A., Parsons, M. S., Olafsen, N. P., Dy, C. J., & Brogan, D. M. (2024) · Journal of the American Academy of Orthopaedic Surgeons · doi:10.5435/jaaos-d-24-00889 · Cited by 6
From resting potential to dynamics: advances in membrane voltage indicators and imaging techniques
Shakibi, R., Yazdipour, F., Abadijoo, H., Manoochehri, N., Pouria, F. R., Bajooli, T., Simaee, H., Abdolmaleki, P., Khatibi, A., Abdolahad, M., et al. (2025) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583524000210 · Cited by 6
Optimization of light delivery by a nanowire-based single cell optical endoscope
Ladanov, M., Cheemalapati, S., & Pyayt, A. L. (2013) · Optics Express · doi:10.1364/oe.21.028001 · Cited by 6
Stent-induced coronary artery stenosis characterized by multimodal nonlinear optical microscopy
Wang, H.-W., Cheng, J., Sturek, M. S., Simianu, V. V., & Locker, M. J. (2011) · Journal of Biomedical Optics · doi:10.1117/1.3533313 · Cited by 6
Cellular and molecular techniques
Bédossa, P., & Paradis, V. (2011) · Elsevier eBooks · doi:10.1016/b978-0-7020-3398-8.00002-7 · Cited by 6
МЕТОДЫ ФОТОНИКИ ДЛЯ ОЦЕНКИ КАЧЕСТВА ПРИЖИВЛЕНИЯ КОЖНЫХ ТРАНСПЛАНТАТОВ
Макаров, И., Ахлюстина, В., Фаррахова, С., Поминова, В., Рябова, В., & Лощенов, Б. (2016) · Biomedical Photonics · doi:10.24931/2413-9432-2016-5-3-30-40 · Cited by 3
Py 3 -FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes
Wang, Z., Mizoguchi, T., Kuribara, T., Nakajima, M., Iwata, M., Sakamoto, Y., Nakamura, H., Murayama, T., Nemoto, T., & Itoh, M. (2021) · Open Biology · doi:10.1098/rsob.200241 · Cited by 6
Crystal structure or chemical composition of salt–sugar-based metal–organic frameworks: what are the nonlinear optical properties due to?
Marabello, D., Antoniotti, P., Benzi, P., Beccari, F., Canepa, C., Cariati, E., Cioci, A., & Presti, L. L. (2021) · Acta Crystallographica Section B Structural Science Crystal Engineering and Materials · doi:10.1107/s2052520621004637 · Cited by 6
Characterisation of carapace composition in developing and adult ostracods (Skogsbergia lerneri) and its potential for biomaterials
Rumney, B. M., Morgan, S. R., Mosselmans, J. F. W., Malik, T., Holden, S. J., Parker, A. R., White, N., Lewis, P. N., Albon, J., & Meek, K. M. (2022) · Marine Biology · doi:10.1007/s00227-022-04047-6 · Cited by 6
Tutorial: multiphoton microscopy to advance neuroscience research
Hernández, I. C., Yau, J., Rishøj, L. S., Cui, N., Minderler, S., & Jowett, N. (2023) · Methods and Applications in Fluorescence · doi:10.1088/2050-6120/acba66 · Cited by 6
Quantifying the microstructural and biomechanical changes in the porcine ventricles during growth and remodelling
Faizan, A., Soe, S. P., Albon, J., Errington, R. J., & Theobald, P. S. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.09.044 · Cited by 6
Label-free quantification of imaging features in the extracellular matrix of left and right-sided colon cancer tissues
Arora, B., Kulkarni, A., Markus, M. A., Ströbel, P., Bohnenberger, H., Alves, F., & Ramos‐Gomes, F. (2024) · Scientific Reports · doi:10.1038/s41598-024-58231-3 · Cited by 8
Highly Sensitive Signal Detection in Stimulated Parametric Emission Microscopy Based on Two-Beam Interferometry
Yamagiwa, M., Ozeki, Y., Kawasumi, T., Kajiyama, S., Fukui, K., & Itoh, K. (2008) · Japanese Journal of Applied Physics · doi:10.1143/jjap.47.8820 · Cited by 6
In vivo polarization dependant Second and Third harmonic generation imaging of Caenorhabditis elegans pharyngeal muscles
Filippidis, G., Troulinaki, K., Fotakis, C., & Tavernarakis, N. (2009) · Laser Physics · doi:10.1134/s1054660x09070184 · Cited by 6
Subcellular Surgery and Nanoneurosurgery Using Femtosecond Laser Pulses
Nuzzo, V., Maxwell, I. Z., Chung, S., Mazur, E., & Heisterkamp, A. (2010) · NATO science for peace and security series. B, Physics and biophysics · doi:10.1007/978-90-481-9977-8_9 · Cited by 6
Patterned-illumination second harmonic generation microscopy of collagen fibrils in rat scleras
Urban, B. E., Dong, B., Zhang, X., Yang, H., & Zhang, H. F. (2018) · Optics Letters · doi:10.1364/ol.43.005190 · Cited by 6
Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy
Sato, K., Matsubara, O., Hase, E., Minamikawa, T., & Yasui, T. (2019) · Journal of Biomedical Optics · doi:10.1117/1.jbo.24.3.031019 · Cited by 6
Developing new SrI2 and β- D -fructopyranose-based metal–organic frameworks with nonlinear optical properties
Marabello, D., Antoniotti, P., Benzi, P., Cariati, E., Presti, L. L., & Canepa, C. (2019) · Acta Crystallographica Section B Structural Science Crystal Engineering and Materials · doi:10.1107/s2052520619001951 · Cited by 6
Large third-order optical nonlinearity and ultrafast optical response in thin Au nanodisks
Yu, Y., Bao, Y., Lin, L., Xu, H., Liu, R., & Zhou, Z. (2019) · Optical Materials Express · doi:10.1364/ome.9.003021 · Cited by 6
Studies on the solvatochromic effect and NLO response in new symmetric bimetallic Rhenium compounds
González, I., Cortés‐Arriagada, D., Dreyse, P., Sanhueza, L., Crivelli, I. G., Ngo, H. M., Ledoux-Rak, I. N., Toro‐Labbé, A., Maze, J. R., & Bárbara Loeb, L. (2020) · Polyhedron · doi:10.1016/j.poly.2020.114679 · Cited by 6
Intravital imaging of orthotopic and ectopic bone
Hashimoto, K., Kaito, T., Kikuta, J., & Ishii, M. (2020) · Inflammation and Regeneration · doi:10.1186/s41232-020-00135-6 · Cited by 6
Multiple quasi-phase-matched second-harmonic generation in phase reversal optical superlattice structure
Meetei, T. S., Ali, M. M., Boomadevi, S., & Pandiyan, K. (2019) · Applied Physics B · doi:10.1007/s00340-019-7255-3 · Cited by 6
Monitoring the morphological evolution of giant vesicles by azo dye-based sum-frequency generation (SFG) microscopy
Momotake, A., Mizuguchi, T., Hishida, M., Yamamoto, Y., Yasui, M., & Nuriya, M. (2019) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2019.110716 · Cited by 6
Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy
Yuan, C., Zhao, X., Wang, Z., Borg, T. K., Ye, T., Khalpey, Z. I., Runyan, R. B., Shao, Y., & Gao, B. Z. (2020) · Biophysical Journal · doi:10.1016/j.bpj.2019.12.030 · Cited by 6
In-vivo corneal nonlinear optical tomography based on second harmonic and multiphoton autofluorescence imaging induced by near-infrared femtosecond lasers with rabbits
Wang, B., Halbhuber, K., Riemann, I., & Koenig, K. (2005) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.622204 · Cited by 6
Simulation study of second-harmonic microscopic imaging signals through tissue-like turbid media
Deng, X., Wang, X., Liu, H., Zhuang, Z., & Guo, Z. (2006) · Journal of Biomedical Optics · doi:10.1117/1.2191055 · Cited by 6
Imaging ofCaenorhabditis eleganssamples and sub-cellular localization of new generation photosensitizers for photodynamic therapy, using non-linear microscopy
Filippidis, G., Kouloumentas, C., Kapsokalyvas, D., Voglis, G., Tavernarakis, N., & Papazoglou, T. G. (2005) · Journal of Physics D Applied Physics · doi:10.1088/0022-3727/38/15/014 · Cited by 6
Visualization can be harmful for live cells
Tárnok, A. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22307 · Cited by 6
Enhancing second harmonic generation by merging symmetry-protected BIC and Fabry–Perot BIC
Zhou, M., Liu, M., Qi, X., & Jiao, Y. (2025) · Applied Optics · doi:10.1364/ao.570065 · Cited by 5
Photonic Crystal Defect Cavities Enable Air‐Stable and Enhanced SHG from NbOCl 2
Bai, L., Jiang, M., Qu, L., Zhu, P., Wu, W., Li, Q., Guo, Z., Sun, X., Huang, Z., Xie, M., et al. (2025) · Advanced Optical Materials · doi:10.1002/adom.202501588 · Cited by 5
GaInSn liquid nanospheres as a saturable absorber for Q-switched pulse generation at 639 nm
Chen, B., Wang, P., Zhang, N., Li, K., Zhang, K., Liu, S., Xu, J., & Sun, Z. (2022) · Optics Express · doi:10.1364/oe.467944 · Cited by 5
Nonlinear microscopy and deep learning classification for mammary gland microenvironment studies
Aghigh, A., Preston, S. E. J., Jargot, G., Ibrahim, H., Rincon, S. V. D., & Légaré, F. (2023) · Biomedical Optics Express · doi:10.1364/boe.487087 · Cited by 5
A 20 MHz Repetition Rate, Sub-Picosecond Ti–Sapphire Laser for Fiber Delivery in Nonlinear Microscopy of the Skin
Krolopp, Á., Fésűs, L., Szipőcs, G., Wikonkál, N. M., & Szipöcs, R. (2024) · Life · doi:10.3390/life14020231 · Cited by 5
Cervical Collagen Network Porosity Assessed by SHG Endomicroscopy Distinguishes Preterm and Normal Pregnancy—A Pilot Study
Liang, W., Liu, Y., Guan, H., Sakulsaengprapha, V., Luby‐Phelps, K., Mahendroo, M., & Li, X. D. (2024) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2024.3472015 · Cited by 5
Determination of the Character of the Interaction of Bioactive Ions with Phospholipid Membranes Using Nonlinear Microscopy Methods
Eremchev, M., & Наумов, А. В. (2025) · Journal of Experimental and Theoretical Physics Letters · doi:10.1134/s0021364024605098 · Cited by 5
How vascularization is reciprocally coupled to chondrogenesis and osteogenesis in bone healing: lessons from the growth plate
Mehl, J., Schmidt‐Bleek, K., Ellinghaus, A., Mundlos, S., Gerhardt, H., Duda, G. N., & Viola Vogel, B. (2025) · Bone · doi:10.1016/j.bone.2025.117595 · Cited by 5
Advances in optical microscopy for food structure characterization
Bonilla, J. C., Via, M. A., Brewer, J., & Clausen, M. P. (2025) · Food Structure · doi:10.1016/j.foostr.2025.100484 · Cited by 5
Monitoring photoaging by use of multiphoton fluorescence and second harmonic generation microscopy
Lin, S., Jee, S., Chan, J.-Y., Wu, R.-J., Lo, W., Tan, H., Lin, W., Chen, J., Young, T., Hsu, C.-J., et al. (2006) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.647009 · Cited by 5
Distinguishing different stages of mouse pregnancy using Second Harmonic Generation images
Yousefi, S., Kehtarnavaz, N., Akins, M. L., Luby‐Phelps, K., & Mahendroo, M. (2010) · Southeastern Symposium on System Theory (SSST) · doi:10.1109/ssst.2010.5442801 · Cited by 5
Multimodal multiphoton microscope with depth scanning
Wu, W., Liu, Q., Brandt, C., & Tang, S. (2022) · Multiphoton Microscopy in the Biomedical Sciences · doi:10.1117/12.2608365 · Cited by 5
Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy
Zhou, L., Jiang, R., Meng, J., Qian, S., Jiang, S., Wang, C., Yang, C., Ding, Z., Shu, Z., & Liu, Z. (2022) · Journal of Innovative Optical Health Sciences · doi:10.1142/s1793545822430052 · Cited by 4

Authors: Campagnola, P. J., & Loew, L. M.
Affiliations: UConn Health (US)
Corresponding author: Loew, Leslie M
ORCID: Campagnola, Paul J
Loew, Leslie M
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2003-10-31
Volume: 21
Issue: 11
Pages: 1356-1360
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt894
OpenAlex: W2077572701
Cited by: 1,326
References: 29
Citations by year: 2026: 37 · 2025: 42 · 2024: 43 · 2023: 50 · 2022: 55 · 2021: 55 · 2020: 67 · 2019: 79 · 2018: 55 · 2017: 67 · 2016: 73 · 2015: 79 · 2014: 61 · 2013: 77 · 2012: 72
FWCI: 3.10
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r21rr013472-02)
Office of Naval Research
National Institute of Biomedical Imaging and Bioengineering (R01EB00196)
National Center for Research Resources (R21 RR13472)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
Keywords: second harmonic generation, nonlinear optical microscopy, collagen imaging, microtubules, myosin, membrane potential, neuronal electrical activity, in vivo imaging
MeSH terms: Action Potentials, Cell Membrane, Cells, Image Enhancement, Membrane Potentials, Proteins, Microscopy, Confocal

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks