The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
STIM1 carboxyl-terminus activates native SOC, Icrac and TRPC1 channels
Open accessAbstract
Receptor-evoked Ca2+ signalling involves Ca2+ release from the endoplasmic reticulum, followed by Ca2+ influx across the plasma membrane. Ca2+ influx is essential for many cellular functions, from secretion to transcription, and is mediated by Ca2+-release activated Ca2+ (Icrac) channels and store-operated calcium entry (SOC) channels. Although the molecular identity and regulation of Icrac and SOC channels have not been precisely determined, notable recent findings are the identification of STIM1, which has been indicated to regulate SOC and Icrac channels by functioning as an endoplasmic reticulum Ca2+ sensor, and ORAI1 (ref. 7) or CRACM1 (ref. 8) — both of which may function as Icrac channels or as an Icrac subunit. How STIM1 activates the Ca2+ influx channels and whether STIM1 contributes to the channel pore remains unknown. Here, we identify the structural features that are essential for STIM1-dependent activation of SOC and Icrac channels, and demonstrate that they are identical to those involved in the binding and activation of TRPC1. Notably, the cytosolic carboxyl terminus of STIM1 is sufficient to activate SOC, Icrac and TRPC1 channels even when native STIM1 is depleted by small interfering RNA. Activity of STIM1 requires an ERM domain, which mediates the selective binding of STIM1 to TRPC1, 2 and 4, but not to TRPC3, 6 or 7, and a cationic lysine-rich region, which is essential for gating of TRPC1. Deletion of either region in the constitutively active STIM1D76A yields dominant-negative mutants that block native SOC channels, expressed TRPC1 in HEK293 cells and Icrac in Jurkat cells. These observations implicate STIM1 as a key regulator of activity rather than a channel component, and reveal similar regulation of SOC, Icrac and TRPC channel activation by STIM1.
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Cites
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A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
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Store-Operated Calcium Channels
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Cited by
Store-Operated Calcium Channels
Parekh, A. B., & Putney, J. W. (2005) · Physiological Reviews · doi:10.1152/physrev.00057.2003 · Cited by 2,089
Store-Operated Calcium Channels
Prakriya, M., & Lewis, R. S. (2015) · Physiological Reviews · doi:10.1152/physrev.00020.2014 · Cited by 1,239
Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI
Hogan, P. G., Lewis, R. S., & Rao, A. (2010) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132550 · Cited by 760
The functional network of ion channels in T lymphocytes
Cahalan, M. D., & Chandy, K. G. (2009) · Immunological Reviews · doi:10.1111/j.1600-065x.2009.00816.x · Cited by 599
Oligomerization of STIM1 couples ER calcium depletion to CRAC channel activation
Luik, R. M., Wang, B., Prakriya, M., Wu, M. M., & Lewis, R. S. (2008) · Nature · doi:10.1038/nature07065 · Cited by 578
Metadata
- Authors
- Huang, G. N., Zeng, W., Kim, J. Y., Yuan, J. P., Han, L., Muallem, S., & Worley, P. F.
- Venue
- Nature Cell Biology
- Published
- 2006-08-13
- Volume
- 8
- Issue
- 9
- Pages
- 1003-1010
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2042342392
- Cited by
- 640
- References
- 19
- FWCI
- 26.26
- Type
- journal article
- Language
- en
- Open access
- green
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