The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction
Open accessAbstract
The spindle assembly checkpoint (SAC) is required to block sister chromatid separation until all chromosomes are properly attached to the mitotic apparatus. The SAC prevents cells from entering anaphase by inhibiting the ubiquitylation of cyclin B1 and securin by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase. The target of the SAC is the essential APC/C activator Cdc20. It is unclear how the SAC inactivates Cdc20 but most current models suggest that Cdc20 forms a stable complex with the Mad2 checkpoint protein. Here we show that most Cdc20 is not in a complex with Mad2; instead Mad2 is required for Cdc20 to form a complex with another checkpoint protein, BubR1. We further show that during the SAC, the APC/C ubiquitylates Cdc20 to target it for degradation. Thus, ubiquitylation of human Cdc20 is not required to release it from the checkpoint complex, but to degrade it to maintain mitotic arrest.
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Cites
The spindle-assembly checkpoint in space and time
Musacchio, A., & Salmon, E. D. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2163 · Cited by 2,227
The anaphase promoting complex/cyclosome: a machine designed to destroy
Peters, J.-M. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1988 · Cited by 1,315
Ditchfield, C., Johnson, V. L., Tighe, A., Ellston, R., Haworth, C., Johnson, T., Mortlock, A., Keen, N., & Taylor, S. S. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200208091 · Cited by 1,194
Sudakin, V., Chan, G. K. T., & Yen, T. J. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200102093 · Cited by 902
Temporal and spatial control of cyclin B1 destruction in metaphase
Clute, P., & Pines, J. (1999) · Nature Cell Biology · doi:10.1038/10049 · Cited by 702
Self-citationBudding Yeast Cdc20: A Target of the Spindle Checkpoint
Hwang, L. H., Lau, L. F., Smith, D. L., Mistrot, C. A., Hardwick, K. G., Hwang, E. S., Amon, A., & Murray, A. W. (1998) · Science · doi:10.1126/science.279.5353.1041 · Cited by 566
Brito, D. A., & Rieder, C. L. (2006) · Current Biology · doi:10.1016/j.cub.2006.04.043 · Cited by 539
Timing and Checkpoints in the Regulation of Mitotic Progression
Meraldi, P., Draviam, V. M., & Sorger, P. K. (2004) · Developmental Cell · doi:10.1016/j.devcel.2004.06.006 · Cited by 469
Geley, S., Kramer, E., Gieffers, C., Gannon, J., Peters, J.-M., & Hunt, T. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.1.137 · Cited by 430
Mad2-Independent Inhibition of APCCdc20 by the Mitotic Checkpoint Protein BubR1
Tang, Z., Bharadwaj, R., Li, B., & Yu, H. (2001) · Developmental Cell · doi:10.1016/s1534-5807(01)00019-3 · Cited by 410
Cited by
The molecular biology of spindle assembly checkpoint signaling dynamics.
Musacchio, A. (2015) · Current Biology · doi:10.1016/j.cub.2015.08.051 · Cited by 778
The Spindle Assembly Checkpoint
Lara-Gonzalez, P., Westhorpe, F. G., & Taylor, S. S. (2012) · Current Biology · doi:10.1016/j.cub.2012.10.006 · Cited by 773
Harley, M. E., Allan, L. A., Sanderson, H. S., & Clarke, P. R. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.112 · Cited by 353
de Bie, P., & Ciechanover, A. (2011) · Cell Death & Differentiation · doi:10.1038/cdd.2011.16 · Cited by 296
Kulukian, A., Han, J. S., & Cleveland, D. W. (2009) · Developmental Cell · doi:10.1016/j.devcel.2008.11.005 · Cited by 246
Metadata
- Authors
- Nilsson, J., Yekezare, M., Minshull, J., & Pines, J.
- Venue
- Nature Cell Biology
- Published
- 2008-11-09
- Volume
- 10
- Issue
- 12
- Pages
- 1411-1420
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2029158215
- Cited by
- 295
- References
- 37
- FWCI
- 14.72
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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